Quantitative determination of serum LDL cholesterol by near-infrared spectroscopy

Liu, Kan‐Zhi; Shi, Minhu; Man, Angela; Dembinski, Thomas C.; Shaw, R. Anthony

doi:10.1016/j.vibspec.2005.04.005

Cited by 40 publications

(31 citation statements)

References 24 publications

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“…Moreover, several studies deal with the feasibility of IR spectroscopy-based analytical methods in clinical and diagnostic analysis [27]. Liu et al [28] proposed a reagent-free method for simultaneous determination of serum cholesterol in HDL and LDL by infrared spectroscopy. More recently, the attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was applied to obtain quantitative information about macromolecular composition in adipose tissue as major fat storage depot, and in liver and muscle, which may store ectopic fat [29].…”

Section: Introductionmentioning

confidence: 99%

Characterization of extracellular vesicles by IR spectroscopy: Fast and simple classification based on amide and C H stretching vibrations

Mihály

Deák

Szigyártó

et al. 2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

137

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Extracellular vesicles isolated by differential centrifugation from Jurkat T-cell line were investigated by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR). Amide and C-H stretching band intensity ratios calculated from IR bands, characteristic of protein and lipid components, proved to be distinctive for the different extracellular vesicle subpopulations. This proposed 'spectroscopic protein-to-lipid ratio', combined with the outlined spectrum-analysis protocol is valid also for low sample concentrations (0.15-0.05 mg/ml total protein content) and can carry information about the presence of other non-vesicular formations such as aggregated proteins, lipoproteins and immune complexes. Detailed analysis of IR data reveals compositional changes of extracellular vesicles subpopulations: second derivative spectra suggest changes in protein composition from parent cell towards exosomes favoring proteins with -turns and unordered motifs at the expense of intermolecular -sheet structures. The IR-based protein-to-lipid assessment protocol was tested also for red blood cell derived microvesicles for which similar values were obtained. The potential applicability of this technique for fast and efficient characterization of vesicular components is high as the investigated samples require no further preparations and all the different molecular species can be determined in the same sample. The results indicate that ATR-FTIR measurements provide a simple and reproducible method for the screening of extracellular vesicle preparations. It is hoped that this sophisticated technique will have further impact in extracellular vesicle research.

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Section: Introductionmentioning

confidence: 99%

Characterization of extracellular vesicles by IR spectroscopy: Fast and simple classification based on amide and C H stretching vibrations

Mihály

Deák

Szigyártó

et al. 2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

137

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“…linolenic, arachidonic, etc.) arising from lipid peroxidation (Severcan et al, 2005;Liu et al, 2002). Interestingly, lipid oxidation is increased in the inflammatory groups, as evidenced by the olefinic =CH band at 3012 cm -1 providing further evidence of the importance of lipid peroxidation in periodontal disease pathogenesis (Tsai et al, 2005;Sheikhi et al, 2001).…”

Section: Molecular Fingerprinting Of Gingivitis Gcf By Mir Spectroscopymentioning

confidence: 85%

“…), and the environment of the bond. In the last fifteen years, IR spectroscopists have taken advantage of this molecular information, in combination with pattern recognition/classification methods, to explore its potential as a powerful tool for the diagnoses of various diseases based upon the spectra of biological fluids, including amniotic fluid, lipid profiles, synovial fluid, saliva and gingival crevicular fluid to predict fetal lung maturity (Liu et al, 1998), diagnose heart disease (Liu et al, 2002) and rheumatoid arthritis (Eysel et al, 1997), assess global diabetes-associated alterations and evaluate periodontal inflammations (Xiang et al, 2010), respectively. The IR spectrum of saliva and GCF is a rich source of information regarding the oral cavity and associated inflammation.…”

Section: Molecular Fingerprinting Of Gingivitis Gcf By Mir Spectroscopymentioning

confidence: 99%

Diagnosis and Monitoring of Gingivitis in vivo Using Non-Invasive Technology - Infrared Spectroscopy

Liu¹,

Xiang²,

Cholakis³

et al. 2011

Gingival Diseases - Their Aetiology, Prevention and Treatment

Self Cite

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“…Only when anti-CEA and anti-AFP are successfully modified on the substrate, it could produce the peaks of C = O, N-H and C-N in FT-IR spectrum simultaneously. As shown in Figure 5, the peak at 1645 cm -1 belonged to C = O stretching vibrations of amide I [43], the peak at 1546 cm -1 belonged to N-H stretching vibrations of amide II [44,45], the peak at 1219 cm -1 belonged to C-N stretching vibrations [46]. It was obvious that amide bonds were formed, which meant that the antibodies were modified on ordered gold nanohoneycomb arrays with success.…”

Section: Characterization Of the Sers Tagsmentioning

confidence: 99%

Multiplexing Determination of Cancer-Associated Biomarkers by Surface-Enhanced Raman Scattering Using Ordered Gold Nanohoneycomb Arrays

Liu

Cao

et al. 2017

Bioanalysis

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Aim:Here, a multiplex surface-enhanced Raman scattering (SERS) based assay for simultaneous quantitation of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) was developed. Methods: SERS tags of nanostars and SERS substrates of nanobowl arrays were functionalized with labeling and capturing antibodies, respectively. In presence of antigens, SERS tags, antigens and SERS substrates formed sandwich structure. Results: The SERS-based technique showed a wide linear range from 0.5 to 100 ng/ml and detection limits were 0.41 and 0.35 ng/ml for CEA and AFP in phosphate-buffered saline buffer, respectively. Analysis results of clinical serum samples using this technique were similar to that shown in phosphatebuffered saline buffer. The LODs were 0.44 and 0.40 ng/ml for CEA and AFP, respectively. Conclusion: The precision and stability of this analysis technique were satisfactory, meanwhile, no obvious cross-reactivity could be found. What's more, it also suggested that this novel multiplex SERS-based technique could be a simple, specific, reliable, sensitive and multiplexed tool for important diagnostic and prognostic applications. Cancer is a latent fatal illness which kills millions of people worldwide, and the number of people dying from cancer continues to rise. If they were detected and cured earlier, many patients who are dying from cancer would have been saved [1,2]. In clinical diagnosis of cancer, detecting tumor biomarkers at the early stage enables early diagnosis of cancer and receives great deal of attentions [3,4]. Among various cancer biomarker detections, quantified detection of serum biomarkers plays a critical role not only in detecting disease at the early stage, but also in tracking disease development after medical treatment [5]. Recently, varieties of immunoassays and immunosensors are devoted to the detection of a single cancer biomarker [6][7][8]. Although they achieved low detection limits, a majority of cancers have more than one biomarker that correlated with their incidence, and some cancer biomarkers are nonspecific to a special cancer, such as α-fetoprotein (AFP) and carcinoembryonic antigen (CEA), CEA and AFP are related to many kinds of cancer, including liver cancer, colorectal cancer, lung cancer, ovarian carcinoma, breast cancer and pancreatic cancer. Moreover, due to biological diversity of man, only one biomarker detection may generate false positive, which is unreliable [9,10]. Therefore, the detection of a single cancer biomarker is inadequate in diagnosing a special cancer. Comparing with the single biomarker test, multiplex immunoassays of cancer biomarkers can promote the screening and diagnosis of some cancer-related illness [11][12][13]. In addition, the simultaneous multiplex assay that detected more than one cancer biomarker in a single experiment possesses remarkable superiorities, such as shorter analytical time, enhanced detecting throughput, higher efficiency, lower detection cost and less sample. Therefore, it would be advantageous to explore an efficient and ultras...

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Quantitative determination of serum LDL cholesterol by near-infrared spectroscopy

Cited by 40 publications

References 24 publications

Characterization of extracellular vesicles by IR spectroscopy: Fast and simple classification based on amide and C H stretching vibrations

Characterization of extracellular vesicles by IR spectroscopy: Fast and simple classification based on amide and C H stretching vibrations

Diagnosis and Monitoring of Gingivitis in vivo Using Non-Invasive Technology - Infrared Spectroscopy

Multiplexing Determination of Cancer-Associated Biomarkers by Surface-Enhanced Raman Scattering Using Ordered Gold Nanohoneycomb Arrays

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