Quantitative Determination of Secoiridoid Glucosides in In Vitro Propagated Plants of Gentiana davidii var. formosana by High Performance Liquid Chromatography

Chueh, Fu‐Shin; Chen, Chung-Chuan; Sagare, Abhay P.; Tsay, Hsin‐Sheng

doi:10.1055/s-2001-10622

Cited by 37 publications

(22 citation statements)

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“…Low shoot multiplication values of G. dinarica were similar to those reported for G. acaulis [3]. For most Gentiana species shoot multiplication requires a somewhat higher, 2.0-2.5 mg l -1 concentration of BA as reported for G. lutea, G. cruciata, G. purpurea, G. acaulis [3], G. kurroo [10], G. davidii [12,13], G. pneumonanthe [21], G. scabra [22], and G. asclepiadea [23]. However, different BA concentrations were recommended even for the same Gentian species, as in case of G. lutea [1].…”

Section: Culture Establishmentsupporting

confidence: 60%

“…Conditions for in vitro propagation have also been well defined for G. punctata [7], G. cruciata, G. acaulis and G. purpurea [3,8,9] G. pneumonanthe [2]. In Chinese folk medicine, Gentiana species are considered as important; therefore, Asian species have also been well investigated (G. kurroo [10]; G. tibetica [11]; G. davidii [12,13]). Many varieties of G. triflora have been investigated due to their ability to flower under in vitro culture conditions [14,15] and their differences in ploidy levels [16].…”

Section: Introductionmentioning

confidence: 99%

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In vitro propagation of Gentiana dinarica Beck.

Vinterhalter

Milošević

Janković³

et al. 2012

Open Life Sciences

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Gentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l−1 BA and 0.1 mg l−1 NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5–1.0 mg l−1 IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.

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Section: Culture Establishmentsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Gentiana dinarica Beck.

Vinterhalter

Milošević

Janković³

et al. 2012

Open Life Sciences

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“…29) BA alone or in combination with NAA in the MS basal medium has been used for the in vitro induction of shoots in the medicinal plants Aconitum carmichaeli, 23) Gentiana scabra, 25) Wasabia japonica, 30) and Gentiana davidii. 31) In preliminary studies, we tested leaf segments, internode segments, and nodes for the induction of shoots. However, no shoot formation in leaf and internode segments was observed (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Polygonum multiflorum THUNB and Quantitative Analysis of the Anthraquinones Emodin and Physcion Formed in in Vitro Propagated Shoots and Plants

Lin

Nalawade

Mulabagal

et al. 2003

Biological & Pharmaceutical Bulletin

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The genus Polygonum is a source of a wide range of phenolic compounds, flavanoids anthraquinones, stilbenes and tannins.1) Many species of Polygonum (Polygonum chinensis,2) Polygonum persicaria, 3) Polygonum viscosum, 4)Polygonum hydropiper 5) ) are valued for their medicinal properties. Polygonum multiflorum THUNB, a perennial vine-like herb, is one of the most important and widely used Chinese medicinal herbs of the family Polygonaceae. The vine is called Ye-Jiao and the root tubers are called Ho-shou-wu, which are used as tonic and in many remedies in traditional Chinese medicine. 6,7) Ethnopharmacologically, it is a tonic for anemia, neurasthenia, and hypercholesterolemia and clinically used for the treatment of coronary heart disease, hyperlipidemia, neurosis, and other diseases commonly associated with aging. 8)The active principles were identified as the antioxidant anthraquinones-emodin and physcion 9) (Fig. 1). Many researchers have demonstrated both in vivo and in vitro antioxidant activity in the crude water extracts of the roots of P. multiflorum. [10][11][12] Myocardial protective effects of an anthraquinone containing extracts of P. multiflorum under ex vivo conditions have been demonstrated. 13,14) Studies on P. multiflorum extracts indicate that it enhances the cellular antioxidant activity, increases the function of superoxide dismutase, significantly inhibits the formation of oxidized lipids, 8) and represses lipid peroxidation in rat heart mitochondria 10,15) and carbon tetrachloride hepatotoxicity 16) in rats. The stilbene components of the roots inhibit lipid peroxidation induced by adenosine 5Ј-diphosphate and nicotinamide adenine dinucleotide phosphate in rat liver microsomes. 17)The aims of our present investigation were 1) to establish a method for complete plant regeneration of P. multiflorum by induction and proliferation of adventitious shoots from nodes; and 2) to analyze the content of the anthraquinonesemodin and physcion in commercially available crude drug (processed underground or stem parts of P. multiflorum) in vitro grown shoots and in vitro propagated plants of P. multiflorum.There are reports on the in vitro propagation of rare, endangered aromatic and medicinal plants. [18][19][20] Tissue culture protocols have been extensively used for the in vitro propagation, germplasm conservation, and production of pharmaceutically important bioactive compounds. 21,22) The quality and quantity of the bioactive compounds in the plants depend on the strain as well as growth place.23) Genetically homogenous plants with uniform contents of secondary metabolites can be obtained by in vitro propagation of plants either by somatic embryogenesis 24) or shoot organogenesis. 25) To date, however, to the best of our knowledge there are no reports on the in vitro propagation of P. multiflorum. Development of a rapid in vitro propagation system for P. multiflorum would help in conserving the germplasm and allowing the commercial cultivation of this medicinally important species. This is also the f...

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“…The biosynthesis of secoiridoids in Gentianaceae plants can be related to the physiological development stage which is affected by plant age, physiology and growing environment of the plant (Piatczak et al 2006;Zhang et al 2010). Chueh et al (2001) reported that the content of gentiopicroside and swertiamarin in the aerial and underground parts of G. davidii var. formosana varied with the age of the in vitro plant.…”

Section: Pcr Analysis Of Transgenic Hairy Rootsmentioning

confidence: 99%

Induction of Gentiana cruciata hairy roots and their secondary metabolites

et al. 2011

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Gentiana cruciata L. (cross gentian) is a medicinal and ornamental plant. The root extracts of this species are known to exhibit many curative properties. The natural Gentiana populations are exposed to great danger because of their uncontrolled usage. In this study, hairy roots from Gentiana cruciata L. stem and leaf explants belonging to three different clones were induced by inoculation with four different Agrobacterium rhizogenes wild strains namely A4, 15834, 8196 and R1000. Induction of the root transformation was significantly dependent on the explant type used. On the other hand, the genotype and bacterial strain had no significant effect on hairy root formation. Hairy root formation percentages of the explants varied between 5.6-33.3% in the stem explants, and between 0.0-6.7% in the leaf explants. Transformations of the hairy roots were confirmed by PCR using rolC specific primers, and revealed the absence of contaminating A. rhizogenes with virC primers. Total of twelve hairy root clones were obtained, and their secondary metabolite content was also analyzed by HPLC. Quantitative results exhibited that gentiopicroside was the most abundant compound in all root samples. Furthermore, metabolites such as loganic acid, swertiamarin, and sweroside were also identified and quantified in the samples.

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Quantitative Determination of Secoiridoid Glucosides in In Vitro Propagated Plants of Gentiana davidii var. formosana by High Performance Liquid Chromatography

Cited by 37 publications

References 0 publications

In vitro propagation of Gentiana dinarica Beck.

In vitro propagation of Gentiana dinarica Beck.

Micropropagation of Polygonum multiflorum THUNB and Quantitative Analysis of the Anthraquinones Emodin and Physcion Formed in in Vitro Propagated Shoots and Plants

Induction of Gentiana cruciata hairy roots and their secondary metabolites

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