Quantitative determination of saccharide in Haemophilus influenzae type b glycoconjugate vaccines, alone and in combination with DPT, by use of high-performance anion-exchange chromatography with pulsed amperometric detection

Development of a high-resolution magic-angle spinning nuclear magnetic resonance identity assay of the capsular polysaccharide from Haemophilus influenzae type b present in cetavlon precipitate

“…Twodimensional TOCSY and NOESY spectra led to the full assignment of the repetitive unit of the polysaccharide through the characteristic scalar connectivities within each moiety and the NOE peak connecting both within the repetitive unit. The obtained assignment of all protons is in agreement with the literature data [12].…”

Formulation and Evaluation of Aluminum Adjuvanted Combination Vaccine with Reduced Freeze Sensitivity

“…C4 cartridge column was used for separating free from total polysaccharide prior to hydrolysis. A Dionex ion chromatography system with Dionex MA-1 column was used [36,37].Abnormal toxicity was performed as per Indian Pharmacopoeia after 3 freeze thaw cycles [38].…”

HPAEC-PAD quantification of Haemophilus influenzae type b polysaccharide in upstream and downstream samples

“…This was carried out essentially as described by Tsai et al [10][11][12]. In short, Hib USP and some specific DSP supernatants were 0.22 m filtered to remove cell debris.…”

“…Preferably, more specific methods should be used [10][11][12][13][14], fit for real-time monitoring. In order to meet these needs, a high performance anion exchange chromatography method with pulsed amperometric detection (HPAEC-PAD) has been developed [15], and evaluated, for its potential to overcome the aforementioned matrix effects.…”

“…In order to meet these needs, a high performance anion exchange chromatography method with pulsed amperometric detection (HPAEC-PAD) has been developed [15], and evaluated, for its potential to overcome the aforementioned matrix effects. Correlation between the results obtained by HPAEC-PAD quantification of the PRP monomeric repeat unit (RU) released by alkaline hydrolysis [10][11][12][13]16], and those from the orcinol colorimetric assay (method adapted from Ashwell [17]) was expected to be high for DSP samples. USP samples which cannot be analyzed directly by the orcinol method due to interference by fermentation medium components (e.g.…”