Quantitative Determination of Pulegone in Pennyroyal Oil by FT-IR Spectroscopy

Abstract

Pulegone constitutes a monoterpene occurring in Mentha species and primarily in Mentha pulegium L. (pennyroyal). A major source of human exposure to pulegone is the use of pennyroyal essential oil in flavorings, confectionery and cosmetics. The rapid quantification of pulegone in hydrodistilled pennyroyal oils (which were also "spiked" to increase the validation range) by Fourier transform infrared spectroscopy (FT-IR) combined with partial least-squares (PLS) regression was evaluated, using the spectral region 1650-1260 cm(-1). Gas chromatography was applied as the reference method for pennyroyal oil samples, which ranged in pulegone content from 157 to 860 mg/mL. The two methods were subjected to statistical tests and proved equivalent in terms of accuracy and reproducibility (99% confidence level). The use of FT-IR spectroscopy could offer a viable alternative to the standard analysis procedures presently applied for quantification of valuable plant substances and could also provide the processing industry with a simple and high-throughput technique for the fast quality check of incoming raw materials such as pennyroyal oils.

Comparative chemotype determination of Lamiaceae plants by means of GC–MS, FT-IR, and dispersive-Raman spectroscopic techniques and GC-FID quantification

“…Distillation lasted 3 h and the essential oil obtained was treated as described by Petrakis et al (2009).…”

“…While in the region we chose to study, this band did not appear, other intense bands appeared, namely at 1373 cm −1 (assigned to C H sym deformation of >CH CH 3 ), at 1286 cm −1 (assigned to C H sym deformation of C (CH 3 ) 2 ), at 1209 cm −1 (attributed to C H deformation vibration), at 1131 and 936 cm −1 (CH 3 rocking vibrations) and at 876 cm −1 (C H wagging vibration and ring vibration). These bands were considered enough to provide a reliable identification of the chemotype (Petrakis et al, 2009;Schulz et al, 2005).…”

A simplified FTIR chemometric method for simultaneous determination of four oxidation parameters of frying canola oil

“…The low value of RMSECV for TPC, CV, CD and CT demonstrated the suitability of the proposed method. FT-IR spectral pre-treatment and data correction was carried out as per reported procedure [44]. The predictability of the models was tested by computing RMSEP according to reported studies [45,46].…”

Radical Scavenging and Disinfectant Effect of Essential Oil From Moroccan Mentha Pulegium

“…They have been extensively screened for their potential use as alternatives for food protection against lipid oxidation, as well as for the research of new antimicrobials to fight multi-drug resistant microorganisms.The genus Mentha includes 20 species that are widely distributed and can be found in many environments. Several published papers have reported the chemical profile of essential oils from M. pulegium of Iran [1], Greece [2], Turkey [3] and Portugal [4]. Moreover, species of Mentha have been used as folk remedy for the treatment of nausea, bronchitis, flatulence, anorexia, ulcerative colitis and liver complaints, due to their anti-inflammatory, carminative, antiemetic, diaphoretic, antispasmodic, analgesic, stimulant, emmenagogue and anticatharrhal activities [5].…”