Quantitative Determination of phosphatidylcholine by an HPLC‐RI system

Abstract

The following describes the quantitative determining method for phosphatidylcholine (PC) using the HPLC-RI system which we have developed. It uses Lichrosorb, Si 60 (10 pro), 4.6 mm X 250 mm as the column and a mobile phase consisting of n-hexane/isopropanol/water ---1:4:1. In this report, we compared data from selected highpurity (60-100 wt%) samples using the HPLC-RI, HPLC-UV and conventional TLC-P methods.Under the conditions we described, the HPLC-UV method was somewhat affected by fatty acid compositions. As a result, there were some inconsistencies in the measured values. However, the HPLC-RI method we propose was applicable to PC from both egg yolk and soybeans. In addition, the HPLC-RI method produced data which correlated well with data from the TLC-P method, and this data was highly accurate and exhibited satisfactory reproducibility.