Quantitative Determination of Paralytic Shellfish Poisoning Toxins in Shellfish Using Prechromatographic Oxidation and Liquid Chromatography with Fluorescence Detection: Interlaboratory Study

Abstract

An interlaboratory study was conducted for the determination of paralytic shellfish poisoning (PSP) toxins in shellfish. The method used liquid chromatography with fluorescence detection after prechromatographic oxidation of the toxins with hydrogen peroxide and periodate. The PSP toxins studied were saxitoxin (STX), neosaxitoxin (NEO), gonyautoxins 2 and 3 (GTX2,3 together), gonyautoxins 1 and 4 (GTX1,4 together), decarbamoyl saxitoxin (dcSTX), B-1 (GTX5), C-1 and C-2 (C1,2 together), and C-3 and C-4 (C3,4 together). B-2 (GTX6) toxin was also included, but for qualitative identification only. Samples of mussels, both blank and naturally contaminated, were mixed and homogenized to provide a variety of PSP toxin mixtures and concentration levels. The same procedure was followed with samples of clams, oysters, and scallops. Twenty-one samples in total were sent to 21 collaborators who agreed to participate in the study. Results were obtained from 18 laboratories representing 14 different countries.

RNA sequencing and de novo assembly of the digestive gland transcriptome in Mytilus galloprovincialis fed with toxinogenic and non-toxic strains of Alexandrium minutum

“…The analysis of the PSTs was performed on the A. minutum cells and soft mussel tissues at the time point T0, before the first feeding dose, T1 (24 hours after the first feeding) and T2, when the maximum bioaccumulation of toxins was supposedly achieved. The PSTs detection was based on pre-column oxidation and High Performance Liquid Chromatography coupled to Fluorescence Detection (HPLC-FLD) according to the protocol AOAC 2005.06 [ 81 ].…”

Source and profile of paralytic shellfish poisoning toxins in shellfish in Daya Bay, South China Sea

“…Each sample was divided into two sub-samples. One sub-sample was extracted in 0.1 N HCl according to the AOAC extraction procedure as described in 2.5.2, and another was extracted in 1% (v/v) acetic acid solution according to Lawrence et al (2004).…”

Marine biotoxins in shellfish – Saxitoxin group

“…The method was described by Lawrence and Menard (1991) and was evaluated in 2002 in an international collaborative study involving 18 laboratories (Lawrence et al, 2004(Lawrence et al, , 2005. It was successfully validated for the toxins GTX1 and GTX4 together, GTX2 and GTX3 together, C1 and C2 together, C3 and C4 together, NeoSTX, STX, dcSTX and GTX5.…”