Quantitative determination of paclitaxel in human plasma using semi-automated liquid–liquid extraction in conjunction with liquid chromatography/tandem mass spectrometry

A Rapid and Sensitive HPLC Method for Quantitation of Paclitaxel in Biological Samples using Liquid-Liquid Extraction and UV Detection: Application to Pharmacokinetics and Tissues Distribution Study of Paclitaxel Loaded Targeted Polymeric Micelles in.....

“…Moreover, the analysis run times were longer than 25 min makes the process laborious and time consuming which is not acceptable in routine HPLC analysis of PTX particularly it's pharmacokinetic and bioequivalence studies. Some other researchers achieved to the lower LOQ for PTX in plasma and tissues following liquid chromatography tandem mass spectrometric method (30)(31)(32). Although, these methods are sensitive and accurate with the short chromatographic time, they involve expensive equipment which is not affordable at most laboratories.…”

“…They used a small volume of plasma sample (100 µl) and intra-and inter-day variation of the assay were within 9.35 and 8.33%, respectively. To the best of our knowledge, protein precipitation of tissue homogenates following liquid chromatographic tandem mass spectrometric (LC-MS) method has been the only successful method for quantification of PTX in tissue samples (30)(31)(32). Though, LC-MS is more responsive and explicit than HPLC-UV, this technique involves expensive equipment which is not affordable at most laboratories.…”

The effect of alkylamine additives on the sensitivity of detection for paclitaxel and docetaxel and analysis in plasma of paclitaxel by liquid chromatography–tandem mass spectrometry

“…1, P1 and P2, respectively), and demonstrate a broad spectrum of action in the treatment of a variety of human neoplastic diseases, including ovarian, breast, lung, brain cancer and melanoma (Wani et al, 1971;Holms et al, 1991;Forastiere, 1993). Although some LC/MS/MS methods have been developed for detection of paclitaxel and docetaxel with LOQs at ng/mL levels using [M+H] + as the precursor ion (Basileo et al, 2003;Guo et al, 2003;Parise et al, 2003), these two compounds were shown in our work to produce somewhat unstable mass spectrometric detection due to the formation of multiple molecular adduct ions ( ). Furthermore, the calibration curve was nonlinear because of the formation of the [2M+Na] + adduct ion at high concentrations (Stefansson et al, 1996).…”

Liquid–liquid extraction of strongly protein bound BMS-299897 from human plasma and cerebrospinal fluid, followed by high-performance liquid chromatography/tandem mass spectrometry

“…Zhang et al [10] reported 96-well LLE for the quantitation of diphenhydramine, desipramine, chlorpheniramine, and trimipramine in rat plasma with a selection of organic solvents. Similar 96-well LLE approaches were also used for the determination of methylphenidate in human plasma [11], paclitaxel in human plasma [12], SU11248 in monkey tissues [13], simvastatin and its ␤-hydroxy acid in human plasma [14], ABT-578 in human blood [15], a novel insulin sensitizer in human plasma [16]. Song reported an LLE, HILIC/MS/MS method for the analysis of omeprazole, and 5-OH omeprazole in human plasma which eliminated both evaporation and reconstitution steps [17].…”