Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry

Liu, Ke; Dai, Xiaojian; Zhong, Dafang; Chen, Xiaoyan

doi:10.1016/j.jchromb.2008.02.002

Cited by 30 publications

(17 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ovomucoid columns have been used for chiral separations of a large number of drugs and chiral solutes [28,118,164,181–184]. In one recent study, an ovomucoid-based CSP was used with LC-MS/MS to separate and analyze the enantiomers of ondansetron and doxazosin in human plasma and to give information on the pharmacokinetics of these agents [185,186]. …”

Section: Chromatographic Studies Of Protein-based Cspsmentioning

confidence: 99%

Chromatographic Studies of Protein-Based Chiral Separations

Zheng

Azaria

et al. 2016

Separations

View full text Add to dashboard Cite

The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used as binding agents to create chiral stationary phases (CSPs) and in the use of chromatographic methods to study these materials and protein-based chiral separations. The supports and methods that have been employed to prepare protein-based CSPs will also be discussed and compared. Specific types of CSPs that are considered include those that employ serum transport proteins (e.g., human serum albumin, bovine serum albumin, and alpha1-acid glycoprotein), enzymes (e.g., penicillin G acylase, cellobiohydrolases, and α-chymotrypsin) or other types of proteins (e.g., ovomucoid, antibodies, and avidin or streptavidin). The properties and applications for each type of protein and CSP will also be discussed in terms of their use in chromatography and chiral separations.

show abstract

Section: Chromatographic Studies Of Protein-based Cspsmentioning

confidence: 99%

Chromatographic Studies of Protein-Based Chiral Separations

Zheng

Azaria

et al. 2016

Separations

View full text Add to dashboard Cite

show abstract

“…The developed method was validated for selectivity, matrix effect, extraction recovery, linearity, precision, accuracy, dilution integrity and stability 13, 14.…”

Section: Methodsmentioning

confidence: 99%

“…Up to now, no enantioselective method has been developed and reported for the quantitative determination of darusentan enantiomers in biological samples. LC‐MS/MS methods based on chiral stationary phases are widely employed for the assays of drug enantiomers in pharmaceutical preparations and biological fluids 13–15. Thus, the present study was to develop and validate a sensitive and convenient bioanalytical assay for the direct analysis of darusentan enantiomers in rat plasma by LC‐MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Determination of darusentan enantiomers in rat plasma by enantioselective liquid chromatography with tandem mass spectrometry using cellulose‐based chiral stationary phase

Shi

et al. 2011

J of Separation Science

View full text Add to dashboard Cite

A sensitive, specific and rapid liquid chromatography-mass spectrometry (LC-MS/MS) method has been developed and validated for enantioselective determination of darusentan enantiomers, orally active potent endothelin-A receptor antagonist, in rat plasma. The plasma samples were pretreated by protein precipitation with methanol and baseline chromatographic separation was performed on a Chiralcel OD-RH column with a mobile phase consisting of acetonitrile/water/formic acid (50:50:0.1, v/v/v) at a flow rate of 0.5 mL/min. The detection was accomplished by multiple-reaction monitoring (MRM) scanning via electrospray ionization (ESI) source operating in the negative ionization mode. The calibration curve was linear over the investigated concentration from 0.500 to 2500 ng/mL (r≥0.995) for each enantiomer using 50 μL of rat plasma. The lower limit of quantitation (LLOQ) for each enantiomer was 0.500 ng/mL. The intra- and inter-day precisions were not more than 10.2% and the accuracy was within the range from -5.4 to 6.3% for darusentan enantiomers. No chiral inversion was observed during the plasma preparation, storage and analysis. The method proved adequate for enantioselective pharmacokinetic studies of darusentan enantiomers after oral administration of three different doses of racemic darusentan.

show abstract

“…Для определения ондансетрона используют жидкостную хроматографию [2][3][4], спектрофотометрию [5], электрофорез [6]. Также для этих целей применяют вольтамперометрию − один из самых популярных методов определения содержания биологически активных соединений в различных объектах.…”

Section: ондансетронunclassified

Determination of Ondansetron at a Carbon Paste Electrode by Derivative Voltammetry

Варченко¹,

Strilets²,

Беликов³

2014

MOCA

View full text Add to dashboard Cite

Methods and objects of chemical analysis, 2014, Vol. 9, No. 3, 139-144 Ондансетрон (рис. 1) -избирательный антагонист серотониновых рецепторов, который применяется для устранения тошноты и рвоты, возникающих вследствие химио-или радиотерапии. Метаболизм ондансетрона в организме человека протекает путем гидроксилирования индольного кольца, за которым следует образование конъюгатов. Около 5% введенной дозы выводится с мочой в неизменном виде [1]. Для определения ондансетрона используют жидкостную хроматографию [2-4], спектрофотометрию [5], электрофорез [6]. Также для этих целей применяют вольтамперометрию − один из самых популярных методов определения содержания биологически активных соединений в различных объектах. Авторами [7] была разработана методика определения ондансетрона в лекарственных препаратах на стационарном ртутном электроде, предел обнаружения составил 1.7 . 10 -8 М. Однако, из-за токсичности ртутного электрода существует тенденция к уменьшению его использования в анализе и созданию новых безопасных электродов. Отмечена электрохимическая активность ондансетрона на стеклоуглеродном электроде [8], но процесс окисления характеризуется медленным переносом электронов, что делает его непригодным для количественных измерений. Модифицирование поверхности стеклоуглеродного электрода многостенными углеродными нанотрубками 139The electrochemical behavior of ondansetron was studied at a tritolyl phosphate -based carbon paste electrode. It was found that ondansetron is irreversibly oxidized with a one electron transfer. In the pH range of 2-4 peak potential and peak height do not depend on pH. When determining ondansetron, 0.1 M sodium sulfate solution was found to be the best supporting electrolyte, providing the lowest detection limit. Based on this study a simple and rapid voltammetric method was developed for ondansetron determination in human urine. Derivative voltammetry was used to improve the resolution and increase the sensitivity. For ondansetron quantification in human urine, nonlinear standard addition method was used; a detection limit was 7 . 10 -7 M. Изучено электрохимическое поведение ондансетрона на угольно-пастовом электроде на основе тритолил фосфата. Установлено, что ондансетрон необратимо окисляется с переносом одного электрона. В интервале pH 2-4 потенциал пика и его высота не зависят от pH среды. Установлено, что при определении ондансетрона 0.1 М раствор сульфата натрия является наилучшим фоновым электролитом, обеспечивающим наиболее низкое значение предела обнаружения. На основании проведенного исследования была разработана простая и быстрая вольтамперометрическая методика определения ондансетрона в моче человека. Для улучшения разрешения и повышения чувствительности был применен метод производной вольтамперометрии. При определении содержания ондансетрона в моче использовали нелинейный метод стандартных добавок; предел обнаружения составил 7 . 10 -7 М. Ключевые слова: ондансетрон, угольно-пастовый электрод, дифференциальная импульсная вольтамперометрия, производная вольтамперометрия

show abstract

Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry

Cited by 30 publications

References 17 publications

Chromatographic Studies of Protein-Based Chiral Separations

Chromatographic Studies of Protein-Based Chiral Separations

Determination of darusentan enantiomers in rat plasma by enantioselective liquid chromatography with tandem mass spectrometry using cellulose‐based chiral stationary phase

Determination of Ondansetron at a Carbon Paste Electrode by Derivative Voltammetry

Contact Info

Product

Resources

About