Quantitative determination of ochratoxin A by liquid chromatography/electrospray tandem mass spectrometry

Lau, Benjamin P.‐Y.; Scott, P. M.; Lewis, David A.; Kanhere, Shriniwas R

doi:10.1002/(sici)1096-9888(200001)35:1<23::aid-jms903>3.0.co;2-b

Cited by 78 publications

(37 citation statements)

References 16 publications

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“…In accordance to literature data (RazzaziFazeli 1999(RazzaziFazeli , 2002Zö llner et al 1999;Lau et al 2000;Lagana et al 2003), electrospray with negative ionization proved to be applicable more widely than with positive ionization. Negatively charged molecular ions of type B-trichothecenes (NIV, DON, ADON, FX) were detected by far more sensitively than their positively charged analogues.…”

Section: Investigations Of Mass Spectrometric Parameterssupporting

confidence: 81%

“…Most common methods for simultaneous determination of A and B-trichothecenes require GC-ECD, GC-MS or LC with post-column derivatization and fluorescence detection (Krska 2001). Recently, LC-MS/MS has been increasingly applied to mycotoxin analysis (Berger et al 1999;Razzazi-Fazeli et al 1999, 2002Zö llner et al 1999;Lau et al 2000, Plattner 2000Lagana et al 2003;Pallaroni & Holst 2003;Royer et al 2004;Berthiller et al 2005). The main advantages are its general applicability, high sensitivity and outstanding selectivity.…”

Section: Introductionmentioning

confidence: 99%

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Development of a multicomponent method forFusariumtoxins using LC-MS/MS and its application during a survey for the content of T-2 toxin and deoxynivalenol in various feed and food samples

Biselli

Hummert²

2005

Food Additives and Contaminants

117

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A reliable, sensitive and selective method was developed to determine different Fusarium mycotoxins (trichothecenes Type A and B, zearalenone) simultaneously in cereals and cereal-based samples using liquid chromatography with tandem mass spectrometry (LC-ESI-MS/MS). Sample preparation is based on a standard solvent extraction step followed by two different kinds of solid-phase clean-up procedures: using a multifunctional MycoSep Õ material for trichothecenes and zearalenone. The average recoveries for trichothecenes ranged from 65% for nivalenol (NIV) up to 96% for deoxynivalenol (DON) and 89% for zearalenone (ZON). The limit of quantification varied between 0.02 and 10 ppb for each substance. In addition, a screening survey with 685 samples was carried out to compare contents of T-2 toxin and deoxynivalenol and to investigate potential coherence in contamination pattern.

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Section: Investigations Of Mass Spectrometric Parameterssupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Development of a multicomponent method forFusariumtoxins using LC-MS/MS and its application during a survey for the content of T-2 toxin and deoxynivalenol in various feed and food samples

Biselli

Hummert²

2005

Food Additives and Contaminants

117

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show abstract

“…These characteristics agree reasonably well with the LC/Fluorescence detection obtained for complex matrices (LAU et al, 2000).…”

Section: Resultssupporting

confidence: 88%

Development and validation of a method for detection and quantification of ochratoxin A in green coffee using liquid chromatography coupled to mass spectrometry

et al. 2012

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A method using Liquid Chromatography Tanden Mass Spectrometry (LC-MS/MS) with matrix-matched calibration curve was developed and validated for determining ochratoxin A (OTA) in green coffee. Linearity was found between 3.0 and 23.0 ng.g-1. Mean recoveries ranged between 90.45% and 108.81%; the relative standard deviation under repeatability and intermediate precision conditions ranged from 5.39% to 9.94% and from 2.20% to 14.34%, respectively. The limits of detection and quantification were 1.2 ng.g-1 and 3.0 ng.g-¹, respectively. The method developed was suitable and contributed to the field of mycotoxin analysis, and it will be used for future production of the Certified Reference Material (CRM) for OTA in coffee.

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“…Multi-mycotoxin analysis by using LC-MS and LC-MS-MS technology was also reported by Rundberget and Wilkins (2002) for determination of several Penicillium mycotoxins, including OTA, in food mixtures. LC/MS/MS was also applied to the OTA analysis of contaminated coffee samples, using a method developed for OTA determination in human plasma, and gave results comparable to those obtained with a conventional LC-FD method (Lau et al 2000). Jorgensen and Vahl (1999) reported a LC-electrospray ionization (ESI)-MS-MS method for OTA quantification in pig kidneys with a detection limit of 0.02 mg/kg, using OTA methyl ester as the internal standard.…”

Section: Other Analytical Methodsmentioning

confidence: 92%

Fitness for purpose – Ochratoxin A analytical developments

Visconti

Girolamo

2005

Food Additives & Contaminants

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Laboratory accreditation involving third party auditing, the use of validated methods and participation in laboratory proficiency testing are essential elements for laboratory quality assurance in relation to ochratoxin A (OTA) analysis. A number of methods, mainly based on liquid chromatography (LC) with fluorescence detection (FD), coupled with immunoaffinity column or solid phase extraction cleanup, have been collaboratively validated and adopted as official standards for OTA determination in a variety of foods, including cereals, coffee, wine and beer. Enzyme-linked immunosorbent assays (ELISA) kits are widely used as screening methods for the occurrence of OTA in food. Novel technologies using anti-OTA antibodies (electrochemical immunosensors, fluorescence polarisation, lateral flow devices, enzyme-based flow through membranes, and surface plasmon resonance biosensors) have been proposed for rapid analysis of OTA in food and beverages, and may be applied for in situ measurements. Validation of these immunochemical methods and commercial kits is required. Liquid chromatography-mass spectrometry represents an adequate alternative to LC-FD particularly in the area of multi-mycotoxin analysis. OTA specific molecularly imprinted polymers are currently considered for cleanup as a potential and cheaper alternative to immunoaffinity or solid-phase extraction sorbents.

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Quantitative determination of ochratoxin A by liquid chromatography/electrospray tandem mass spectrometry

Cited by 78 publications

References 16 publications

Development of a multicomponent method forFusariumtoxins using LC-MS/MS and its application during a survey for the content of T-2 toxin and deoxynivalenol in various feed and food samples

Development of a multicomponent method forFusariumtoxins using LC-MS/MS and its application during a survey for the content of T-2 toxin and deoxynivalenol in various feed and food samples

Development and validation of a method for detection and quantification of ochratoxin A in green coffee using liquid chromatography coupled to mass spectrometry

Fitness for purpose – Ochratoxin A analytical developments

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