Quantitative determination of ochratoxin A in wine after the clarification and filtration

Durguti, Valon; Georgieva, Aneliya; Angelov, Angel; Bajrami, Zyri

doi:10.17508/cjfst.2014.6.2.03

Cited by 4 publications

(1 citation statement)

References 16 publications

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“…The chromatographic separation was performed using a reversed-phase column (Extend-C18, Zorbax column, 4.6 mm i.d., 250 mm, 5 μm, Agilent Co.), in which the mobile phase should be sufficiently transparent at the wavelength of detection. The mobile phase used was water: acetonitrile: methanol (60: 20: 20, v:v:v) (Durguti et al 2014 ). The fluorescent detector was set to a wavelength of 330 nm for excitation and 460 nm for emission.…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of ochratoxin A by endophytic Trichoderma koningii strains

Ismaiel

Mohamed

El-Sayed

2022

World J Microbiol Biotechnol

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Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus spp. and Penicillium spp. that causes a threat to food safety and human health. Fungal biodegradation might be a promising strategy for reducing the OTA contamination in the future. In this study, the ability of Trichoderma koningii strains to degrade OTA produced by Aspergillus niger T2 (MW513392.1) isolated from tomato seeds was investigated. Among T. koningii strains tested, three strains; AUMC11519, AUMC11520 and AUMC11521 completely eliminated OTA from the culture medium, while AUMC11522 strain eliminated only 41.82% of OTA. OTα-amide, 3-phenylpropionic acid, OTα and phenylalanine were assayed as degradation products by FTIR analysis and LC–MS/MS spectra. Carboxypeptidase A (CPA) was found responsible for OTA degradation when a metal ion chelator, EDTA, was added to cell free supernatants of the three effective strains. OTA detoxification by T. koningii could present new prospective strategies for a possible application in food commodities intoxicated with ochratoxin.

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Section: Methodsmentioning

confidence: 99%