Quantitative Determination of Lignans and Isoflavonoids in Plasma of Omnivorous and Vegetarian Women by Isotope Dilution Gas Chromatographγ-Mass Spectrometry

Adlercreutz, Herman; Fotsis, Theodore; Lampe, Johanna W.; Wähälä, Kristiina; Mäkelä, Taru; Brunow, Gösta; Hase, Tapio

doi:10.3109/00365519309090693

Cited by 265 publications

(163 citation statements)

References 40 publications

Supporting

Mentioning

149

Contrasting

Unclassified

Order By: Relevance

“…The range in ENL concentration is largely explained by differences in dietary intakes (Adlercreutz et al, 1987(Adlercreutz et al, , 1993(Adlercreutz et al, , 1994. The range of ENL concentrations is 0.5-82 nmol/l in the present study.…”

Section: Discussionmentioning

confidence: 40%

See 1 more Smart Citation

Variation in fasting and non-fasting serum enterolactone concentrations in women of the Malmö Diet and Cancer cohort

et al. 2007

View full text Add to dashboard Cite

Objectives: The aim of this study was to examine the variation of enterolactone from fasting and non-fasting blood of middleaged healthy women eating a normal diet to determine the usefulness of a single sample in epidemiological studies. Subjects and methods: Twenty-six women born between 1940 and 1950 were recruited within the Malmö Diet and Cancer cohort. Three non-fasting and two overnight fasting samples were collected from each individual during a 5-week period. Twenty-one participated in all measurements. Enterolactone concentrations were analyzed by time-resolved fluoroimmunoassay. Results: The within-subject and between-subject variations (coefficient of variations, CV) were estimated to 59 and 89% respectively for fasting samples and 71 and 67% for non-fasting samples. The intraclass correlation coefficients (ICC) were estimated to 0.66 (95% confidence interval (CI) 0.35-0.84) for fasting and 0.48 (95% CI, 0.22-0.72) for non-fasting samples. Conclusions: Although the estimated ICC for blood samples was moderate, it indicates that enterolactone levels of both fasting and non-fasting blood samples should be useful in future projects within the Malmö Diet and Cancer cohort.

show abstract

Section: Discussionmentioning

confidence: 40%

“…Different bacteria are needed for the conversion of plant lignans into END and ENL (Clavel et al, 2005). ENL is generally the lignan with highest concentration in blood (Adlercreutz et al, 1993) and also the lignan that mostly have been examined in epidemiological studies.…”

Section: Discussionmentioning

confidence: 99%

Variation in fasting and non-fasting serum enterolactone concentrations in women of the Malmö Diet and Cancer cohort

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The largest dietary dosage of phytoestrogenic activity results from two classes of compounds: lignans and isoflavones. These groups modulate many biologically relevant processes, including sex hormone production and metabolism (Adlercreutz et al, 1987(Adlercreutz et al, , 1988, intracellular enzyme levels (Adlercreutz et al, 1993), protein synthesis, malignant cell proliferation, cellular differentiation (Adlercreutz and Mazur, 1997;Kurzer and Xu, 1997), and oxidative balance (Phipps et al, 1993;Wei et al, 1995). Although the physiologic relevance of some of these properties is questionable, consumption of foods rich in phytoestrogens is associated with significantly reduced risk for cancer, osteoporosis, cardiovascular disease, and severity of menopause (Adlercreutz and Mazur, 1997;Kurzer and Xu, 1997).…”

Section: Introductionmentioning

confidence: 99%

Urinary and serum concentrations of seven phytoestrogens in a human reference population subset

Valentín-Blasini

Blount

Caudill

et al. 2003

J Expo Sci Environ Epidemiol

View full text Add to dashboard Cite

Diets rich in naturally occurring plant estrogens (phytoestrogens) are strongly associated with a decreased risk for cancer and heart disease in humans. Phytoestrogens have estrogenic and, in some cases, antiestrogenic and antiandrogenic properties, and may contribute to the protective effect of some diets. However, little information is available about the levels of these phytoestrogens in the general US population. Therefore, levels of phytoestrogens were determined in urine (N ¼ 199) and serum (N ¼ 208) samples taken from a nonrepresentative subset of adults who participated in NHANES III, 1988-1994. The phytoestrogens quantified were the lignans (enterolactone, enterodiol, matairesinol); the isoflavones (genistein, daidzein, equol, Odesmethylangolensin); and coumestrol (urine only). Phytoestrogens with the highest mean urinary levels were enterolactone (512 ng/ml), daidzein (317 ng/ ml), and genistein (129 ng/ml). In serum, the concentrations were much less and the relative order was reversed, with genistein having the highest mean level (4.7 ng/ml), followed by daidzein (3.9 ng/ml) and enterolactone (3.6 ng/ml). Highly significant correlations of phytoestrogen levels in urine and serum samples from the same persons were observed for enterolactone, enterodiol, genistein, and daidzein. Determination of phytoestrogen concentrations in large study populations will give a better insight into the actual dietary exposure to these biologically active compounds in the US population.

show abstract

“…Although it is known that isoflavones are consistently high in the serum and urine of humans who regularly consume soy protein foods (Adlercreutz et al, 1991(Adlercreutz et al, , 1993bMorton et al, 1994;Murphy et al, 1982;Setchell et al, 1997Setchell et al, , 1998, it is less well appreciated in the scientific community that commercial diets formulated with soy meal (Drane et al, 1975;Murphy, 1982;Thigpen et al, 1999aThigpen et al, , 1999b) cause rodents to have sustained high steady-state serum isoflavone concentrations. Such effects have tremendous implications for animal experiments, particularly when biochemical or morphological end-points are being measured that may be influenced by the hormonal or nonhormonal properties of isoflavones (Boettger-Tong et al, 1998;Drane et al, 1975).…”

mentioning

confidence: 99%

“…For example, when adults consume modest amounts of soy protein foods, the serum and urinary concentrations of these isoflavones typically exceed endogenous estradiol concentrations by several orders of magnitude (Adlercreutz et al, 1993b;Setchell, 1998;Setchell and Cassidy, 1999). Similarly, infants fed soy formula have serum isoflavone levels that exceed estradiol by 13,000-to 22,000-fold (Setchell et al, 1997;Setchell et al, 1998).…”

mentioning

confidence: 99%

Animal Models Impacted by Phytoestrogens in Commercial Chow: Implications for Pathways Influenced by Hormones

Brown

Setchell

2001

Laboratory Investigation

271

239

View full text Add to dashboard Cite

SUMMARY:It is generally not known that most commercial rodent diets are formulated with soy protein and deliver large daily doses of isoflavones to animals throughout their lifespan, including the in utero period. Here, we demonstrate that isoflavones are bioavailable and show that commercial rodent diets universally used by animal facilities lead to very high steady-state serum isoflavone concentrations in adult rats (2613 Ϯ 873 ng/mL) and mice (2338 Ϯ 531 ng/mL), exceeding the animal's endogenous estrogen level by 30,000-to 60,000-fold. We demonstrate the maternal-fetal intrauterine transfer of isoflavones in animals fed a standard Purina 5001 soy-containing diet and show that newborn rat pups have high serum isoflavones levels (540 Ϯ 174 ng/mL) that are maintained throughout the suckling period by passage of isoflavones into maternal milk. These findings have profound implications for all animal experiments, including multigenerational studies and studies of transgenic animals, especially if biochemical or morphological end-points are influenced by the hormonal or nonhormonal properties of phytoestrogens. These compounds have the potential to modulate genotypic and phenotypic expression in general, and therefore, all investigators should be vigilant to the phytoestrogen composition of commercial rodent diets because there is a history of potent biological effects in larger animals and in humans from high circulating isoflavone concentrations. (Lab Invest 2001, 81:735-747).A lthough phytoestrogens are ubiquitous in the plant kingdom, it is soybeans and foods made with purified soy proteins that are by far the greatest contributors of isoflavones to animal and human diets (Coward et

show abstract

Quantitative Determination of Lignans and Isoflavonoids in Plasma of Omnivorous and Vegetarian Women by Isotope Dilution Gas Chromatographγ-Mass Spectrometry

Cited by 265 publications

References 40 publications

Variation in fasting and non-fasting serum enterolactone concentrations in women of the Malmö Diet and Cancer cohort

Variation in fasting and non-fasting serum enterolactone concentrations in women of the Malmö Diet and Cancer cohort

Urinary and serum concentrations of seven phytoestrogens in a human reference population subset

Animal Models Impacted by Phytoestrogens in Commercial Chow: Implications for Pathways Influenced by Hormones

Contact Info

Product

Resources

About