Quantitative determination of in Vivo endocytosis by rat liver kupffer and endothelial cells facilitated by an improved cell isolation method

Dalen, Danielle P. Praaning-Van; Knook, D.L.

doi:10.1016/0014-5793(82)80054-9

Cited by 59 publications

(19 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings are at variance with results of a previous study by Summerfield in which 1251-AGOR was preferentially taken up by endothelial cell-enriched fractions, but not by Kupffer cell-enriched fractions (5), and also with the results of an electron microscopic autoradiographic study which indicated that relatively more AGOR and mannose-terminated orosomucoid are taken up by endothelial cells than by Kupffer cells (19). Furthermore, Praaning-van Dalen reported that ligands recognized by the mannose/GlcNAc receptor are taken up by both Kupffer and endothelial cells in uiuo as well as in uitro (20). This controversy emphasizes the crucial importance of carefully characterizing the populations of cells present in different elutriator fractions.…”

Section: Endothelial Cells Can Degrade Hyaluronic Acid (Bmentioning

confidence: 86%

Hepatic Sinusoidal Cells: New Insights and Controversies

Jones

1983

Hepatology

View full text Add to dashboard Cite

Section: Endothelial Cells Can Degrade Hyaluronic Acid (Bmentioning

confidence: 86%

Hepatic Sinusoidal Cells: New Insights and Controversies

Jones

1983

Hepatology

View full text Add to dashboard Cite

“…However, TEM examination revealed that approximately 80% of the cells in Fraction 2 were endothelial, and approximately 80% of the cells in Fraction 4 were Kupffer. Praaning-Van Dalen and Knook, using a similar isolation technique, reported the average purity of endothelial and Kupffer cell fractions from rat liver was 90 and 80%, respectively (3).…”

Section: Discussionmentioning

confidence: 98%

Sinusoidal Endothelial Cells from Normal Guinea Pig Liver: Isolation, Culture and Characterization†

et al. 1984

View full text Add to dashboard Cite

Guinea pig nonparenchymal hepatic cells were isolated by enzymatic digestion and subsequent separation on a 17.5% metrizamide gradient. Endothelial cell and Kupffer cell-enriched fractions were separated by centrifugal elutriation. Viability of both cell fractions was approximately 80%. Endothelial cells were cultured on a substratum of guinea pig liver collagen and 1% gelatin (1:1). Freshly isolated and cultured sinusoidal endothelial cells contained Factor VIII R:antigen, angiotensin I converting enzyme activity, and they synthesized prostaglandins characteristic of other endothelial cells. Sieve plates were identified in both freshly isolated and cultured cells. Fresh endothelial cells and Kupffer cells formed Fc receptor-mediated rosettes with IgG-opsonized sheep red blood cells, but cultured endothelial cells did not. Only Kupffer cells demonstrated Fc and C3 receptor-mediated phagocytosis. These methods for isolating and culturing sinusoidal endothelial cells should permit further functional assessment of endothelial cells and their interrelationship with other sinusoidal lining cells.

show abstract

“…KCs were isolated using a collagenase perfusion method as previously described [1] . Briefly, after anesthesia (30 mg of barbital /kg body wt., intraperitoneally), the liver was perfused in situ with Ca was then removed and placed in buffer.…”

Section: Isolation Of Kcmentioning

confidence: 99%

“…Aged Kupffer cells (KCs) have a decreased phagocytic capacity [1] . A recent study found that alterations of cellular energy metabolism is one of the main causes responsible for the disordered cellular function [2] .…”

Section: Introductionmentioning

confidence: 99%

Protective effect of vitamin E on age-related alterations of Kupffer cell energy metabolism

Sun¹,

Ma²,

Peng³

et al. 2011

WJG

View full text Add to dashboard Cite

AIM:To investigate the mechanism of age-related reduction of Kupffer cell (KC) phagocytic capacity and the protective management. METHODS:Using rhodamine 123 fluorescence density and rate of glucose utilization as parameters, we measured the mitochondrial energy metabolism status in vitro and the glucose utilization capacity of isolated rat liver Kupffer cells (KCs) from rats of various ages (6 mo, 12 mo, 18 mo and 24 mo) and the effect of vitamin E (VE) pretreatment (500 mg/kg/wk × 13 wk). RESULTS:The rate of KC glucose utilization and the rhodamine fluorescence density of KC mitochondria of 18 mo-old untreated rats (NVEG) were significantly lower than that of 6 mo-old NVEG by 19.3% (4.0 nmol·h ± 0.4 nmol·h -1 10.6 cells -1 vs 5.7 nmol·h ± 0.6 nmol·h -1 10 6 cells -1 , P < 0.05) and 19.5% (80.5 ± 6.3 vs 100.0 ± 4.7, P < 0.01) respectively; Rate of KC glucose utilization and the rhodamine fluorescence density of KC mitochondria of 6 mo-old rats were also lower than the 24 mo-old NVEG by 35.1% (3.7 nmol·h ± 0.6 nmol·h , P < 0.01) and 32.1% (67.9 ± 7.4 vs 100.0 ± 4.7, P < 0.01) respectively.The two parameters of 18 mo-old VE pretreated rats (VEG) were significantly higher than those of 18 mo-old NVEG, and statistically comparable to those of 6 mo-old VEG. The two parameters of the 24 mo-old VEG were significantly higher in comparison with those of 24 mo-old NVEG, but still significantly lower than those of 6 mo-old VEG.CONCLUSION: Aging has a significantly negative effect on KC energy metabolism, which can be alleviated by VE pretreatment. Sun WB, Ma RL, Peng ZM, Li K, Duan HC, Han BL. Protective effect of vitamin E on age-related alterations of Kupffer cell energy metabolism. World

show abstract

Quantitative determination of in Vivo endocytosis by rat liver kupffer and endothelial cells facilitated by an improved cell isolation method

Cited by 59 publications

References 11 publications

Hepatic Sinusoidal Cells: New Insights and Controversies

Hepatic Sinusoidal Cells: New Insights and Controversies

Sinusoidal Endothelial Cells from Normal Guinea Pig Liver: Isolation, Culture and Characterization†

Protective effect of vitamin E on age-related alterations of Kupffer cell energy metabolism

Contact Info

Product

Resources

About