Quantitative determination of glycine in commercial dosage forms by kinetic spectrophotometry

Mitić, Snežana S.; Pavlović, Aleksandra; Tošić, Snežana B.; Arsić, Biljana; Sunarić, Slavica

doi:10.1134/s1061934809070053

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…Thus, we argue that a glycine-detection mission should aim for a minimal detection capability of ≈1 × 10 −7 mol L −1 . Mitić et al (2009) report on a kinetic spectrophotometry method with Note. Note that these ranges can be adjusted to account for biases in Gly concentration in material found in the space plume relative to the concentration in the ocean plume.…”

Section: Bayesian Hypothesis Selection For Amino Acid Detectionmentioning

confidence: 99%

Putative Methanogenic Biosphere in Enceladus's Deep Ocean: Biomass, Productivity, and Implications for Detection

Affholder¹,

Guyot²,

Sauterey³

et al. 2022

Planet. Sci. J.

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Saturn's moon Enceladus is a top candidate in the search for extraterrestrial life in our solar system. Ecological thermodynamic modeling of the plume composition data collected by NASA's Cassini mission led to the hypothesis that a hydrogenotrophic methanogenic ecosystem might exist in the putative hydrothermal vents at Enceladus's seafloor. Here we extend this approach to quantify the ecosystem's expected biomass stock and production and evaluate its detectability from the collection of plume material. We find that although a hypothetical biosphere in Enceladus's ocean could be small (<10 tons of carbon), measurable amounts of cells and organics might enter the plume. However, it is critical that missions be designed to gain meaningful insights from a negative outcome (no detection). We show that in order to sample a cell from the plume with 95% confidence, >0.1 mL of material needs to be collected. This would require material from more than 100 fly-bys through the plume or using a lander. We then consider amino acid abundance as an alternative signature and find that the absolute abundance of amino acids, such as glycine, could be very informative if a detection threshold of 1 × 10−7 mol L−1 could be achieved. Altogether, our findings set relatively high bars on sample volume and amino acid detection thresholds, but these goals seem within the reach of near-future missions.

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Section: Bayesian Hypothesis Selection For Amino Acid Detectionmentioning

confidence: 99%

Putative Methanogenic Biosphere in Enceladus's Deep Ocean: Biomass, Productivity, and Implications for Detection

Affholder¹,

Guyot²,

Sauterey³

et al. 2022

Planet. Sci. J.

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show abstract

“…[5] In addition, Gly oligomers, that is, short oligopeptides are important biomolecules due to their broad applications in the study of protein binding events [6] and enzymatic activities. [7] Furthermore, all AAs, except Gly, have a chiral carbon and can be found in either levorotary (L-) or dextrorotary (D-) forms.…”

Section: Introductionmentioning

confidence: 99%

Selective and direct detection of free amino acid using the optical birefringent patterns of confined nematic liquid crystals

Wei

Jang

2016

Liquid Crystals

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Detection of amino acid (AA) is an essential step to understand various biological processes. In this study, we used innovative optical birefringent patterns of nematic liquid crystals (LCs) for the detection of several AAs. We attempted to use capillary-confined nematic LCs as sensor for AA analysis because their three-dimensional micro-scale architecture of LCs allowed better performance than that of mainly reported LC thin film sensors owing to the effect on the formation and dynamics of point defect. The sensing system was built by disrupting the alignment of a nematic LC, 4-cyano-4′-pentylbiphenyl (5CB), using the dopant of dodecyl aldehyde. Detection principle is based on the chemical analytical method of aldehyde titration for AAs, wherein the reaction between AAs and aldehyde group generates Schiff bases that could alter the configuration of nematic LCs at the aqueous/LC interface. The patterns generated in the reaction are captured by polarising optical microscopy (POM) and are visible to the naked eye. The functionalised LCs detected glycine at concentrations as low as 1 pM. There was a surprising result that it can selectively detect D-AAs against their L-isomers, however, further efforts are required to explain the mechanism.ARTICLE HISTORY

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Quantitative Determination of Glycine in Aqueous Solution Using Glutamate Dehydrogenase-Immobilized Glyoxal Agarose Beads

Keskin

2013

Appl Biochem Biotechnol

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In this study, an enzymatic procedure for the determination of glycine (Gly) was developed by using a column containing immobilized glutamate dehydrogenase (GDH) on glyoxal agarose beads. Ammonia is produced from the enzymatic reactions between Gly and GDH with NAD(+) in phosphate buffer medium. The indophenol blue method was used for ammonia detection based on the spectrophotometric measurements of blue-colored product absorbing at 640 nm. The calibration graph is linear in the range of 0.1-10 mM of Gly concentrations. The effect of pH, temperature, and time interval was studied to find column stability, and also the interference effects of other amino acids was investigated. The interaction between GDH and glyoxal agarose beads was analyzed by Fourier transform infrared (FTIR) spectroscopy. The morphology of the immobilized and non-immobilized agarose beads were characterized by atomic force microscopy (AFM).

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Quantitative determination of glycine in commercial dosage forms by kinetic spectrophotometry

Cited by 4 publications

References 17 publications

Putative Methanogenic Biosphere in Enceladus's Deep Ocean: Biomass, Productivity, and Implications for Detection

Putative Methanogenic Biosphere in Enceladus's Deep Ocean: Biomass, Productivity, and Implications for Detection

Selective and direct detection of free amino acid using the optical birefringent patterns of confined nematic liquid crystals

Quantitative Determination of Glycine in Aqueous Solution Using Glutamate Dehydrogenase-Immobilized Glyoxal Agarose Beads

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