Quantitative determination of five ergot alkaloids in rye flour by liquid chromatography–electrospray ionisation tandem mass spectrometry

Development and Validation of an Analytical Method for Determination of Ergot Alkaloids in Animal Feedingstuffs with High Performance Liquid Chromatography-fluorescence Detection

“…For the separation of ergot alkaloids most authors used reverse phase C18 columns (Mohamed et al 2006, Storm et al 2008, Di Mavungu et al 2012 though, the separation was also performed on a C6 phenyl column (Muller et al 2009). As far as the mobile phase is concerned, the most popular composition was ammonium carbonate at different concentrations and acetonitrile (Storm et al 2008, Muller et al 2009, Bryła et al 2015 used in an isocratic and gradient mode as well.…”

Development and validation of a new LC–MS/MS method for the simultaneous determination of six major ergot alkaloids and their corresponding epimers. Application to some food and feed commodities

“…A number of the methods mentioned above have been used for biomedical application (Friedrich et al, 2004) and /or for identification purposes (Lehner et al, 2005). Some include no or only few -inine isomers (Burk et al, 2006;Kokkonen & Jestoi, 2010;Mohamed et al, 2006), possibly because of the lack of available standards. reported an LC-MS/MS multi-analyte method including both the -ine as well as the -inine isomers of the six most common ergot alkaloids, which was validated for ten different cereal and food samples.…”

“…It is therefore important to consider both epimers when the ergot alkaloid contamination level of a cereal or cereal product has to be determined. Different methods have been reported for the analysis of ergot alkaloids, including thin layer chromatography (TLC) (Salvat & Godoy, 2001), capillary electrophoresis (CE) (Franch & Blaschke, 1998), enzyme linked immunosorbent assay (ELISA) (Hill et al, 2001), gas chromatography (GC) with electron capture detection (ECD) (Barrow & Quigley, 1975), liquid chromatography (LC) with ultraviolet (Veress, 1993), fluorescence (Komarova & Tolkachev, 2001;Storm et al, 2008) or mass spectrometric (MS) (Burk et al, 2006;Kokkonen & Jestoi, 2010;Krska, Stubbings, Macarthur, & Crews, 2008;Mohamed, Gremaud, Rychoz-Payot, Tabet, & Guy, 2006) detection. In recent years, LC-MS has become the method of choice for mycotoxin determination (Shephard et al, 2011;Spanjer, 2010), and has provided an unequivocal identification of ergot alkaloids in various matrices (Friedrich et al, 2004;Lehner, Craig, Fannin, Bush, & Tobin, 2005;Mohamed et al, 2006).…”

An integrated targeted and untargeted approach for the analysis of ergot alkaloids in cereals using UHPLC – hybrid quadrupole time-of-flight mass spectrometry

“…Analytical methods for the determination of ergot alkaloids include thin layer chromatography (TLC) (Salvat and Godoy, 2001), capillary electrophoresis (CE) (Franch and Blaschke, 1998), enzyme linked immunosorbent assay (ELISA) (Hill et al, 2001), gas chromatography (GC) with electron capture detection (ECD) (Barrow and Quigley, 1975), liquid chromatography (LC) with ultraviolet (Veress, 1993), fluorescence (Komarova and Tolkachev, 2001a;Storm et al, 2008) or mass spectrometric (MS) (Diana di Mavungu et al, 2012;Burk et al, 2006;Kokkonen and Jestoi, 2010;Krska et al, 2008a;Mohamed et al, 2006b) detection. Over the last decade, LC-MS has become a dominant tool for mycotoxin determination, and has provided an unequivocal identification of ergot alkaloids in various matrices (Diana di Mavungu et al, 2012;Friedrich et al, 2004;Kokkonen and Jestoi, 2009;Krska et al, 2008;Lehner et al, 2005;Mohamed et al, 2006a;Mohamed et al, 2006b). Due to its simple operation and low cost, ergot alkaloid analysis is currently mainly conducted using a triple-quadrupole (QqQ) MS system.…”