Quantitative determination of fifteen basic pharmaceuticals in ante- and post-mortem whole blood by high pH mobile phase reversed phase ultra high performance liquid chromatography–tandem mass spectrometry

Amundsen, I.; Øiestad, Åse Marit Leere; Ekeberg, Dag; Kristoffersen, Lena

doi:10.1016/j.jchromb.2012.12.039

Cited by 46 publications

(34 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It shows high sensitivity and has a wide concentration range that includes sub-therapeutic to toxic quetiapine blood levels. The developed method when compared to previously published methods shows similar or better validation results, such as similar [37] or lower LOQ values [6,18,21,32,33] and relative [21,33] or wider linearity range [6,18]. The reliability of the method was also certified by means of a validation study and its successful application for the toxicological investigation of 50 quetiapine-related fatal cases.…”

Section: Discussionmentioning

confidence: 67%

See 1 more Smart Citation

A validated GC/MS method for the investigation of quetiapine-related deaths in Greece

Papoutsis¹,

Katselou²,

Nikolaou³

et al. 2017

Eur J Forensic Sci

View full text Add to dashboard Cite

Objective: Quetiapine is an atypical antipsychotic drug that is used to treat the symptoms of schizophrenia and other psychiatric disorders. During the last years, quetiapine has also been associated with cases of misuse and abuse, and there are strong indications that it possesses an addictive potential. A suitable method is necessary for the toxicological investigation of such cases. Materials and Methods: A simple gas chromatographymass spectrometric method was developed, validated, and applied for the determination of quetiapine in biological fluids during the investigation of quetiapine-related cases. The sample preparation procedure includes solid-phase extraction and silylation using N-methyl-N-tert-butyldimethylsilyl-trifluoroacetamide with 1% tert-butyldimethylsilylchloride. Results: The limits of detection and quantification were 1.50 and 5.00 ng/mL, respectively. The calibration curves were linear up to 2000 ng/mL (R 2 ≥ 0.991) and absolute recovery higher than 81%. Accuracy (intra-and inter-day) was found to be between −11.1 and 12.4%, whereas the precision was found to be <13.3%. The validated method was successfully applied to post-mortem blood samples during the toxicological investigation of 50 quetiapine-related sudden and violent deaths. Quetiapine was determined at toxic levels (>1800 ng/mL) in eight cases and at lower concentrations (5.21-1430 ng/mL) in the other 42 cases. It is remarkable to be mentioned that in all 50 cases one or more drugs were detected along with quetiapine. Conclusions: Quetiapine has been detected with increasing frequency in post-mortem cases in Greece during the last 3 years. This is in accordance with the growing concerns worldwide about the misuse or abuse of the drug and its contribution to the cause of death of quetiapine-related lethal cases. Physicians should be alert about the toxicity of quetiapine, its possible misuse or abuse, and its synergy with other drugs, medicinal or not, especially in patients under multiple drug therapy or drug addicts.

show abstract

Section: Discussionmentioning

confidence: 67%

“…Several methods have been already published for the determination of quetiapine in biological samples using LC [37][38][39][40][41] or GC methods [6,18,21,32,33,42,43]. The aim of this work was to investigate quetiapine-related lethal cases to assess the contribution of quetiapine to the cause of death.…”

Section: Discussionmentioning

confidence: 99%

A validated GC/MS method for the investigation of quetiapine-related deaths in Greece

Papoutsis¹,

Katselou²,

Nikolaou³

et al. 2017

Eur J Forensic Sci

View full text Add to dashboard Cite

show abstract

“…The LODs and the volumes of specimens in the previous reports from 2009 to present are as follows [2][3][4][5][6][7][8]. In a GC study, the LODs of several ADs were 2.4-15 ng/mL using 0.1 mL of plasma [absolute amounts (AAs) 0.24-1.5 ng] [2].…”

Section: Comparison Of Limits Of Detection Of the Present Maldi-ms Mementioning

confidence: 96%

“…Many fatal cases involving these compounds have been reported [1]. The detection methods of ADs reported in several journals related to clinical or forensic toxicology, from 2009 to present, were gas chromatography (GC) [2], GC-mass spectrometry (MS) [3,4], liquid chromatography (LC)-tandem mass spectrometry (MS/MS) [5,6] and ultra-performance (UP) LC-MS/MS [7,8].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of cyclic antidepressants and their related drugs and the estimation of new metabolites in human whole blood and urine by MALDI-QTOF-mass spectrometry

et al. 2016

View full text Add to dashboard Cite

A rapid and sensitive method using matrix-assisted laser desorption ionization (MALDI)-quadrupole time-of-flight (QTOF)-mass spectrometry (MS) was developed for the analysis of six tricyclic antidepressants (ADs) and their related drugs, such as amitriptyline, carbamazepine, clomipramine, imipramine, nortriptyline, quetiapine, and two tetracyclic ADs, mianserin and mirtazapine, because these eight drugs are commonly observed medicines in poisoning cases in Japan. In the present MALDI-MS method, the limits of detection were 0.3-2 ng/ mL. The present method provided a simple and high throughput simultaneous analysis of these drugs. This is the first report dealing with quantitation of ADs and their related drugs in biological samples by MALDI-MS. In addition, we applied the MALDI-MS to two authentic cases, and three new metabolites of amitriptyline and three new metabolites of quetiapine could be detected in blood and urine samples in the two cases when regioisomers were counted as one metabolite.

show abstract

“…A limited number of analytical methods are sensitive enough for directly identifying drug molecules in biological samples [31]. As biological fluids have a complex composition, a biological sample pre-treatment before applying the method for quantitative evaluation is required to remove proteins and potential interferences [11].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Corina¹,

Hăncianu²,

Agoroaei³

et al. 2015

Acta Medica Marisiensis

View full text Add to dashboard Cite

Arterial hypertension is a complex disease with many serious complications, representing a leading cause of mortality. Selective beta-blockers such as metoprolol and bisoprolol are frequently used in the management of hypertension. Numerous analytical methods have been developed for the determination of these substances in biological fluids, such as liquid chromatography coupled with mass spectrometry, gas chromatography coupled with mass spectrometry, high performance liquid chromatography. Due to the complex composition of biological fluids a biological sample pre-treatment before the use of the method for quantitative determination is required in order to remove proteins and potential interferences. The most commonly used methods for processing biological samples containing metoprolol and bisoprolol were identified through a thorough literature search using PubMed, ScienceDirect, and Willey Journals databases. Articles published between years 2005-2015 were reviewed. Protein precipitation, liquid-liquid extraction and solid phase extraction are the main techniques for the extraction of these drugs from plasma, serum, whole blood and urine samples. In addition, numerous other techniques have been developed for the preparation of biological samples, such as dispersive liquid-liquid microextraction, carrier-mediated liquid phase microextraction, hollow fiber-protected liquid phase microextraction, on-line molecularly imprinted solid phase extraction. The analysis of metoprolol and bisoprolol in human plasma, urine and other biological fluids provides important information in clinical and toxicological trials, thus requiring the application of appropriate extraction techniques for the detection of these antihypertensive substances at nanogram and picogram levels.

show abstract

Quantitative determination of fifteen basic pharmaceuticals in ante- and post-mortem whole blood by high pH mobile phase reversed phase ultra high performance liquid chromatography–tandem mass spectrometry

Cited by 46 publications

References 47 publications

A validated GC/MS method for the investigation of quetiapine-related deaths in Greece

A validated GC/MS method for the investigation of quetiapine-related deaths in Greece

Simultaneous determination of cyclic antidepressants and their related drugs and the estimation of new metabolites in human whole blood and urine by MALDI-QTOF-mass spectrometry

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

Contact Info

Product

Resources

About