Quantitative determination of EDTA and its behaviour in radioactive waste solutions using HPLC

Unger, Martina; Mainka, E.; König, W.

doi:10.1007/bf00487541

Cited by 13 publications

(5 citation statements)

References 7 publications

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“…There are many competing phenomena that determine which EDTA chelate is most readily formed . While copper and iron have both been used for the analytical determination of EDTA in water, − nickel has a stability constant similar to copper…”

Section: Methodsmentioning

confidence: 99%

Quantitative Capillary Electrophoresis/Ion Spray Tandem Mass Spectrometry Determination of EDTA in Human Plasma and Urine

Sheppard

Henion

1997

Anal. Chem.

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A quantitative method has been developed for the determination of EDTA in human plasma and urine. The samples are prepared with automated anion-exchange solid-phase extraction using 100 microL of human plasma. The extracts are analyzed by capillary electrophoresis/mass spectrometry using selected reaction monitoring in the negative ion mode. Large-volume injections (10% of the CE capillary volume) are used to improve the concentration level of detection via field-amplified sample injection. The first reported validation of a CE/MS/MS technique was carried out for this method. Using a 13C stable-label isotope for the internal standard, the lower level of detection and lower level of quantitation were determined to be 7.3 and 14.6 ng/mL EDTA in human plasma, respectively. The injection precision had a relative standard deviation (RSD) of 6.1%. The intra-assay precision was less than 15% RSD. The intra-assay accuracy was less than +/- 12% bias from the nominal concentration. The interassay precision was less than 18% RSD and the interassay accuracy was less than +/- 9% bias from the nominal concentration.

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Section: Methodsmentioning

confidence: 99%

Quantitative Capillary Electrophoresis/Ion Spray Tandem Mass Spectrometry Determination of EDTA in Human Plasma and Urine

Sheppard

Henion

1997

Anal. Chem.

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“…Detection limits were at 1 µ% L-1 («3.5 nM) for sample volumes of 20 mL. Some samples were analyzed by a HPLC procedure directly after irradiation according to earlier reports (32)(33)(34). Fe(III)-EDTA was measured as an ion pair with tetrabutylammonium bromide by means of absorbance at = 258 nm after chromatographic separation on a RP-Cis column (LiChrospher 100,5µ , 250 x 4 mm).…”

Section: Introductionmentioning

confidence: 99%

Determination of the Reaction Quantum Yield for the Photochemical Degradation of Fe(III)-EDTA: Implications for the Environmental Fate of EDTA in Surface Waters

Kari¹,

Hilger²,

Canonica³

1995

Environ. Sci. Technol.

135

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“…[CuEDTA] 2-within the pH-range of 4-10 (pH 8.2 in our case) and this chelating reaction has a fairly high equilibrium constant (Log K = *18-21) [41]. Judging from the order of reactions, the mechanisms proposed by others might be somewhat different from ours.…”

Section: Kinetic Analysis Of Gsh Oxidationmentioning

confidence: 81%

Kinetic studies of the oxidation of glutathione in protein refolding buffer

Wang

Hung

Lin

2009

Bioprocess Biosyst Eng

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Here, we examined the change of glutathione (GSH) under different conditions and determined the appropriate kinetic schemes to describe its change of concentration. GSH was continuously oxidized into glutathione disulfide (GSSG) as incubation period increased at the temperatures ranging from 10 to 50 degrees Celsius while certain oxidation by-products were also observed at the later stage of reaction at higher temperatures (70-90 degrees Celsius). The addition of 0.3 mM GSSG in 3 mM GSH solution delayed the onset of GSH oxidation without significantly changing the rate of GSH oxidation. Our results also revealed that GSH oxidation could be facilitated upon the addition of copper (II) ion whereas GSH oxidation was found to be decelerated when EDTA was present. In kinetic analysis, the reaction orders of GSH oxidation under various conditions were determined. Moreover, the temperature dependence of the rate of GSH oxidation was modeled by Arrhenius and Eyring equations and resultant activation parameters were also presented.

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Quantitative determination of EDTA and its behaviour in radioactive waste solutions using HPLC

Cited by 13 publications

References 7 publications

Quantitative Capillary Electrophoresis/Ion Spray Tandem Mass Spectrometry Determination of EDTA in Human Plasma and Urine

Quantitative Capillary Electrophoresis/Ion Spray Tandem Mass Spectrometry Determination of EDTA in Human Plasma and Urine

Determination of the Reaction Quantum Yield for the Photochemical Degradation of Fe(III)-EDTA: Implications for the Environmental Fate of EDTA in Surface Waters

Kinetic studies of the oxidation of glutathione in protein refolding buffer

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