Quantitative determination of drug encapsulation in poly(lactic acid) nanoparticles by capillary electrophoresis

A, Helle; Hirsjärvi, Samuli; Peltonen, Leena; Hirvonen, Jouni; Wiedmer, Susanne Κ.

doi:10.1016/j.chroma.2007.11.041

Cited by 36 publications

(33 citation statements)

References 24 publications

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“…On-line testing of the recovery was not a feasible option, as injection of standards directly to the vessel would not give realistic results, because the standards would be directly eluted from the vessel. In our previous studies, off-line dissolution was proven to give quantitative results [18]. Assuming full dissolution by the off-line method, quantitative dissolution was obtained (103 and 120%) with the dynamic system utilizing fully organic solvent (100% methanol), for BDP and indomethacin, respectively.…”

Section: Quantitative Resultsmentioning

confidence: 99%

“…PLA nanoparticles were prepared by the nanoprecipitation method [19] as described in our previous publication [18]. Briefly, PLA and BDP or indomethacin were dissolved in acetone and precipitated as nanoparticles in water.…”

Section: Nanoparticlesmentioning

confidence: 99%

“…In addition, forces created by ultracentrifugation or ultrafiltration might cause unwanted particle destruction and drug release in the medium. In our previous study, an off-line system utilizing capillary electromigration techniques for the determination of the total amount of drug in PLA nanoparticles was developed [18]. In that study, both charged and hydrophilic drugs were studied, including beclomethasone dipropionate (BDP).…”

Section: Introductionmentioning

confidence: 99%

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Novel, dynamic on-line analytical separation system for dissolution of drugs from poly(lactic acid) nanoparticles

Hirsjärvi

Peltonen

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

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Section: Quantitative Resultsmentioning

confidence: 99%

Section: Nanoparticlesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel, dynamic on-line analytical separation system for dissolution of drugs from poly(lactic acid) nanoparticles

Hirsjärvi

Peltonen

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

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“…Both drugs are co-formulated in a nasal spray dosage form and are widely used for effective treatment of nasal congestion and allergy. Several methods have been reported for the determination of SCG in pharmaceutical preparations such as spectrophotometry [3,4], electrophoresis [5], electrochemical [6,7] and HPLC methods [8][9][10][11][12][13][14]. OXMT has also been analyzed by several methods in pharmaceutical preparations such as spectrophotometry [3,[15][16][17][18][19][20][21][22][23], capillary electrophoresis [24,25], gas chromatography [26,27] and HPLC [28][29][30][31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Spectral resolution and simultaneous determination of oxymetazoline hydrochloride and sodium cromoglycate by derivative and ratio-based spectrophotometric methods

et al. 2015

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Sodium cromoglycate (SCG) and oxymetazoline hydrochloride (OXMT) are administered in combination for effective treatment of nasal congestion and allergy. In this work, SCG was determined using direct spectrophotometry by measuring its zero order absorption spectra at its λmax 320.6 nm where OXMT showed zero absorbance. On the other hand, four simple, sensitive and precise spectrophotometric methods were developed and validated for the determination of OXMT in the presence of SCG in their laboratory prepared mixtures and pharmaceutical formulation, without preliminary separation; Method A: first derivative spectrophotometric method [ 1 D], Method B: first derivative of ratio spectra method [ 1 DD], Method C: ratio difference spectrophotometric method [RDSM] and Method D: ratio subtraction method [RSM]. Ratio manipulating methods (Method B, C and D) were done using divisor of 10.00 µg/mL SCG. Linear correlation was obtained in range 4-22 µg/mL for OXMT by methods A, B and D and 6-22 µg/mL for method C. All methods were validated in compliance with the International Conference on Harmonization (ICH) guidelines and satisfactory results were obtained. No significant difference was noted between the developed methods and the official one with respect to accuracy and precision.

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“…Following powder aerosol administration, BD is partially removed from its primary site of action, the lower airways, by mucociliary clearance prior to dissolution and absorption, and eventually, swallowed into the gastrointestinal tract (Hochhaus et al, 1997). Therefore, BD entrapment within biocompatible and biodegradable nanocarriers is a promising approach to improve therapeutic efficacy, reduce the risk of side effects by prolonging BD retention time in the lung and achieve controlled or targeted drug delivery (Helle et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating RP-HPLC method for determination of beclomethasone dipropionate in nanocapsule suspensions

Chassot

Ferreira

Gomes

et al. 2015

Braz. J. Pharm. Sci.

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A simple stability-indicating RP-HPLC/UV method was validated for determination of beclomethasone dipropionate (BD) in nanocapsule suspensions. Chromatographic conditions consisted of a RP C 18 column (250 mm x 4.60 mm, 5 µm, 110 Å), using methanol and water (85:15 v/v) as mobile phase at 1.0 mL/ min with UV detection at 254 nm. The calibration curve was found to be linear in the concentration range of 5.0-25.0 µg/mL with a correlation coefficient > 0.999. Precision was demonstrated by a relative standard deviation lower than 2.0%. Accuracy was assessed by the recovery test of BD from nanocapsules (98.03% to 100.35%). Specificity showed no interference from the components of nanocapsules or from the degradation products derived from acid, basic and photolytic conditions. In conclusion, the method is suitable to be applied to assay BD in bulk drug and in nanocapsules, and it can be employed to study stability and degradation kinetics.Uniterms: High performance liquid chromatography/reverse phase/quantitative analysis. Beclomethasone dipropionate/determination. Nanocapsule/suspensions. Nanoparticles.Um método simples de CLAE-FR/UV indicativo de estabilidade foi validado para a determinação do dipropionato de beclometasona (BD) em suspensões de nanocápsulas. As condições cromatográficas foram: coluna C18 fase reversa (250 mm x 4,60 mm, 5 µm, 110 Å), usando como fase móvel metanol e água (85:15 v/v) a 1,0 mL/min, com detecção UV a 254 nm. A curva de calibração foi linear no intervalo de 5,0-25,0 µg/mL com coeficiente de correlação >0,999. A precisão foi demonstrada por um desvio padrão relativo menor que 2,0%. A exatidão foi avaliada pelo teste de recuperação do BD a partir das nanocápsulas (98,03% a 100,35%). O teste de especificidade não mostrou interferência dos componentes das nanocápsulas e nem dos produtos de degradação derivados de condições ácidas, básicas e fotolíticas. Em conclusão, o método é adequado para ser aplicado na avaliação do BD puro e em nanocápsulas e pode ser empregado para o estudo de estabilidade e degradação cinética.Unitermos: Cromatografia líquida de alta eficiência/fase reversa/análise quantitativa. Dipropionato de beclometasona/determinação. Nanocápsulas/suspensões. Nanopartículas.

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Quantitative determination of drug encapsulation in poly(lactic acid) nanoparticles by capillary electrophoresis

Cited by 36 publications

References 24 publications

Novel, dynamic on-line analytical separation system for dissolution of drugs from poly(lactic acid) nanoparticles

Novel, dynamic on-line analytical separation system for dissolution of drugs from poly(lactic acid) nanoparticles

Spectral resolution and simultaneous determination of oxymetazoline hydrochloride and sodium cromoglycate by derivative and ratio-based spectrophotometric methods

Stability-indicating RP-HPLC method for determination of beclomethasone dipropionate in nanocapsule suspensions

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