Quantitative determination of donepezil in human plasma by liquid chromatography/tandem mass spectrometry employing an automated liquid–liquid extraction based on 96-well format plates

Apostolou, Constantinos; Dotsikas, Yannis; Kousoulos, Constantinos; Loukas, Yannis L.

doi:10.1016/j.jchromb.2006.10.037

Cited by 42 publications

(28 citation statements)

References 17 publications

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“…The liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for the analysis of donepezil and memantine with minor modifications [10,11]. For the quantification of donepezil and memantine, an isocratic mobile phase containing 70% acetonitrile with 0.1% formic acid and 30% aqueous 10 m M ammonium acetate containing 0.1% formic acid was used.…”

Section: Methodsmentioning

confidence: 99%

Impact of CYP2D6 and CYP3A4 Genetic Polymorphism on Combined Cholinesterase Inhibitors and Memantine Treatment in Mild to Moderate Alzheimer's Disease

Nirmal

Tripathi²,

Sagar³

et al. 2013

Dement Geriatr Cogn Disord

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Aim: The impact of CYP2D6 and CYP3A4 polymorphism on the steady-state plasma concentrations and therapeutic outcome of donepezil monotherapy and combination therapy in Alzheimer's disease (AD) patients. Methods: A total of 38 patients for donepezil and 17 patients for donepezil and memantine therapy, aged ≥55 years, were recruited meeting inclusion and exclusion criteria. Polymerase chain reaction-restriction fragment length polymorphism was performed. The liquid chromatography-tandem mass spectrometry method was used for estimation of drug levels of donepezil and memantine. Results: Significant allele frequency was observed for CYP2D6*3 polymorphism in patients on donepezil monotherapy and combination therapy. Significant allele frequency for CYP2D6*4 was observed in the patients on donepezil monotherapy. Conclusion: CYP2D6 polymorphism, though not significant, might partially be involved in the plasma concentration of AD drug.

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Section: Methodsmentioning

confidence: 99%

Impact of CYP2D6 and CYP3A4 Genetic Polymorphism on Combined Cholinesterase Inhibitors and Memantine Treatment in Mild to Moderate Alzheimer's Disease

Nirmal

Tripathi²,

Sagar³

et al. 2013

Dement Geriatr Cogn Disord

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“…ME profiles for the whole chromatographic run were investigated by the application of the post-column infusion protocol. Blank plasma extracts were injected in the LC-MS/MS system by the simultaneous post-column infusion of a mixture of the analyte and the IS (standard three concentration level) in mobile phase via the Harvard syringe pump [23,24]. The flow rate was set at 20 mL/min, whereas the syringe pump was connected in parallel with the ionization source via a PEEK tee.…”

Section: Stability Studiesmentioning

confidence: 99%

Validation of a novel LC‐MS/MS method for the quantitation of colistin A and B in human plasma

Dotsikas

Markopoulou

Koundourellis

et al. 2010

J of Separation Science

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A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method, characterized by complete automation and high-throughput, was developed for the determination of colistin A and B in human plasma. All sample preparation procedures were performed by using 2.2 mL 96-deep-well plates, whereas robotic liquid-handling workstations were utilized for all liquid transfer steps, including solid-phase extraction (SPE). The whole preparation procedure was very rapid, whereas the method had a very short chromatographic run time of just 2 min. Sample analysis was performed by reversed phase LC-MS/MS, with positive electrospray ionization, using multiple reaction monitoring. The absence of available purified colistin A and B standards led to the development of a novel LC method with evaporative light-scattering detector for the determination of their stoichiometries in the standard mixture, along with its purity. The proposed bioanalytical method was fully validated and it was proven to be selective, accurate, precise, reproducible and suitable for the determination of colistin A and B in human plasma. It was applied successfully to a pharmacokinetic study for the determination of both analytes in samples of patients.

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“…For the determination of donepezil in human plasma, number of methods based on high-performance liquid chromatography (HPLC) (Yasui-Furukori et al, 2002;Mihara et al, 1993;Mahasen et al, 2006;Lee et al, 1992;Matsui et al, 1999a;Haginaka and Scyama, 1992;Nakashima et al, 2006) and liquid chromatographymass spectrometry (Matsui et al, 1995(Matsui et al, , 1999b(Matsui et al, , 2006Lu Y et al, 2004;Hao et al, 2003;Apostolou et al, 2007) have been described in recent years. The majority of these methods are based on liquidliquid extraction (LLE) using isopropyl alcohol:hexane (3:97, v/v).…”

Section: Introductionmentioning

confidence: 99%

“…The modern drug development process requires a simple, efficient and rugged extraction procedure, with small analytical run time and high sensitivity. The sensitive method reported (Apostolou et al, 2007) utilizes LC-MS/MS with a run time of 2 min and LLE by hexane. This method was used robotic liquid handling workstations employed for all liquid transfer and solution preparation steps.…”

Section: Introductionmentioning

confidence: 99%

A rapid and specific approach for direct measurement of donepezil concentration in human plasma by LC‐MS/MS employing solid‐phase extraction

Shah

Kundlik

Pandya

et al. 2008

Biomedical Chromatography

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A selective, rapid and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is described for assay of donepezil in human plasma using escitalopram as an internal standard. Chromatographic separation was achieved on a Betabasic-C(8), 5 microm, 100 x 4.6 mm column using methanol:water:formic acid (90:9.97:0.03, v/v/v) as mobile phase. Detection of donepezil and internal standard was achieved by ESI MS/MS in positive ion mode using 380.20/91.10 and 325.13/262.00 transitions, respectively. The linearity over the concentration range of 0.15-50 ng/mL for donepezil was obtained and the lower limit of quantification was 0.15 ng/mL. For each level of quality control samples, inter-day and intra-day precisions (RSD) were < or =8.92 and 10.35% and accuracy (%RE) were < or =7.33% and 9.33%, respectively. The recovery was more than 88.50% for both donepezil and internal standard by solid-phase extraction, eliminating evaporation and reconstitution steps.

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Quantitative determination of donepezil in human plasma by liquid chromatography/tandem mass spectrometry employing an automated liquid–liquid extraction based on 96-well format plates

Cited by 42 publications

References 17 publications

Impact of CYP2D6 and CYP3A4 Genetic Polymorphism on Combined Cholinesterase Inhibitors and Memantine Treatment in Mild to Moderate Alzheimer's Disease

Impact of CYP2D6 and CYP3A4 Genetic Polymorphism on Combined Cholinesterase Inhibitors and Memantine Treatment in Mild to Moderate Alzheimer's Disease

Validation of a novel LC‐MS/MS method for the quantitation of colistin A and B in human plasma

A rapid and specific approach for direct measurement of donepezil concentration in human plasma by LC‐MS/MS employing solid‐phase extraction

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