Quantitative determination of copro- and uroporphyrin in urine

Reinkingh, W.A.; Kampen, E. van

doi:10.1016/0009-8981(64)90130-5

Cited by 10 publications

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…71 Other reported values of urinary porphyrins in 0.1 N HCl include e = 541 000 M À1 cm À1 for uroporphyrin and e = 485 000 M À1 cm À1 for coproporphyrin. 73 Absorption data have been obtained in 1.5 M HCl for the series of five porphyrins with unit increments in the number of carboxylic acid groups from coproporphyrin (400 nm, e = 489 000 M À1 cm À1 ) to uroporphyrin (406 nm, e = 541 000 M À1 cm À1 ). 74 No absorption data in acidic media were found for etioporphyrin or mesoporphyrin IX.…”

Section: Spectral Propertiesmentioning

confidence: 99%

A tandem combinatorial model for the prebiogenesis of diverse tetrapyrrole macrocycles

et al. 2012

View full text Add to dashboard Cite

The extant biosynthesis of tetrapyrrole macrocycles has been considered a paradigm for the prebiotic formation of such molecules, yet only a few analogous non-enzymic reactions along the overall process have been demonstrated. In a prior study, the aqueous non-enzymic reaction of a dione and an aminoketone (d-aminolevulinic acid) afforded uroporphyrinogen, Nature's last universal precursor to all extant tetrapyrroles. Here, in one flask the non-enzymic combinatorial reaction of two diones (substituents = acetic acid and methyl) and two aminoketones (substituents = propionic acid and ethyl) yields four pyrroles, which upon subsequent combinatorial reaction afford a distribution of porphyrinogens. A software program for virtual library generation predicts 538 porphyrinogens from this [2 Â 2] reaction (owing to combinations and permutations) of which there are 25 sets of isomers based on condensed formulas of substituents. The collection spans the entire range of polarity enabled by the biosynthesis including uro-(number of carboxylic acids = 8), copro-(4), meso-( 2), and etio-porphyrinogen (0). The first two are successive intermediates in the extant biosynthesis, the latter two resemble in polarity the advanced biosynthetic products protoporphyrin and chlorophyll. Upon consideration of substituent patterns, the porphyrins (obtained by oxidation of the porphyrinogens) can be grouped into one of four polarity categories (predicted percentage): hydrophilic (0.4%), uncertain (83.6%), amphiphilic (15.6%), and hydrophobic (0.4%). HPLC and mass spectrometry data are consistent with expectations to the limit of analytical capabilities. Thus, in terms of the polarity of the tetrapyrrole macrocycles formed, an all-at-once non-enzymic combinatorial process recapitulates features of the stepwise biosynthetic pathway.

show abstract

Section: Spectral Propertiesmentioning

confidence: 99%

A tandem combinatorial model for the prebiogenesis of diverse tetrapyrrole macrocycles

et al. 2012

View full text Add to dashboard Cite

show abstract

Koproporphyrin [Biomonitoring Methods in German language, 1978b]

Schaller¹

2012

The MAK‐Collection for Occupational Health and Safety

View full text Add to dashboard Cite

Veröffentlicht in der Reihe Analytische Methoden zur Prüfung gesundheitsschädlicher Arbeitsstoffe: Analysen in biologischem Material , 2. Lieferung, Ausgabe 1978 Der Artikel enthält folgende Kapitel: Zusammenfassung Geräte, Chemikalien und Lösungen Geräte Chemikalien Lösungen Probenahme und Probenaufbereitung Eichung Analytische Bestimmung Berechnung des Analysenergebnisses Beurteilung des Verfahrens Präzision Richtigkeit Nachweisgrenze Störeinflüsse Diskussion Koproporphyrin

show abstract

Biochemical methods for the detection of lead poisoning

Kisser

1977

Arch. Toxicol.

View full text Add to dashboard Cite

After a short description of sources of lead poisoning a review is given of toxicity, absorption, storage and excretion of lead. Afterwards its biochemical affects--especially the influence on the biosynthesis of heme--are treated. Methods for diagnosis of lead poisoning resulting from these affects--determination of coproporphyrine and delta-aminolevulinic acid excretion in urine, erythrocyte delta-aminolevulinic acid dehydratase activity and erythrocyte protoporphyrin--are discussed in detail.

show abstract

Quantitative determination of copro- and uroporphyrin in urine

Cited by 10 publications

References 3 publications

A tandem combinatorial model for the prebiogenesis of diverse tetrapyrrole macrocycles

A tandem combinatorial model for the prebiogenesis of diverse tetrapyrrole macrocycles

Koproporphyrin [Biomonitoring Methods in German language, 1978b]

Biochemical methods for the detection of lead poisoning

Contact Info

Product

Resources

About