Quantitative determination of cholesterol, sitosterol, and sitostanol in cultured Caco-2 cells by liquid chromatography–atmospheric pressure chemical ionization mass spectrometry

Determination of free cholesterol based on a novel flow‐injection chemiluminescence method by immobilizing enzyme

“…These methods for cholesterol determination were mostly based on the indirect determination of products in the enzymatic reaction (8,9) or depletion of oxygen (10). Various analytical methods have been reported for cholesterol determination, including colorimetric (11), spectrophotometric (12) and amperometric methods (13)(14)(15)(16)(17)(18), potentiometry (19), voltammetry (20), electrophoresis (21), capillary gas-liquid chromatography (22)(23)(24), quartz crystal acoustic wave sensor (25), enzymatic endpoint method (26), near-infrared spectroscopy (27), fluorometric (28), optical biosensor (29)(30)(31)(32) and chemiluminescence (CL) methods (33)(34)(35). Amperometric methods have often been documented as the biosensor, while the major disadvantages of these sensors were the need for calibration of the sensor both before and after the measurement, the short lifetime of the sensor and interferences of other electrochemically active species (such as ascorbic and uric acids).…”

Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC–ESI–MS/MS

“…Since sterols are less polar and relatively low molecular weight compounds, APCI [26][27][28][29][30][31][32][33] or APPI [20,34] have been preferentially used for analysis by HPLC-MS. The detection limits of cholesterol precursors and sitosterol by HPLC-APCI-MS were well below 1 pmol [31], that of cholesterol by HPLC-APCI-MS/MS was 2.2 pmol [32], and those of oxysterols by HPLC-APCI-MS were in the range of 0.2-0.8 ng (about 0.5-2.0 pmol) [26] or 0.1-0.75 ng (about 0.25-1.9 pmol) [27].…”

Simultaneous determination of plasmatic phytosterols and cholesterol precursors using gas chromatography–mass spectrometry (GC–MS) with selective ion monitoring (SIM)

“…Another GC-MS method was described by Chevy et al [16] for determination of cholesterol, lathosterol, 8-dehydrocholesterol, and 7-dehydrocholesterol from amniotic fluid. LC-APCI-MS was used to identify sitosterol and stigmasterol in soybean oil [17] and to quantify sitosterol and sitostanol in cultured CaCo-2 cells [18].…”

“…Several methods for sterol analysis from various biological matrixes have been described, including high performance liquid chromatography (HPLC) [7][8][9], gas liquid chromatography (GLC) [10][11][12], gas chromatography coupled to mass spectrometry (GC-MS) [13][14][15][16], and liquid chromatography coupled to mass spectrometry (LC-MS) [17,18].…”