Quantitative Determination of Cardiolipin in Mitochondrial Electron Transferring Complexes by Silicic Acid High-Performance Liquid Chromatography

Gomez, Baltazar; Robinson, N.

doi:10.1006/abio.1998.2998

Cited by 36 publications

(27 citation statements)

References 28 publications

(30 reference statements)

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“…Some investigators use absorbance at 206 nm to quantitate a particular PPL (23,24). However, UV absorbance at 206 nm is largely dependent upon the presence of unsaturated double bonds present in component acyl-groups (22).…”

Section: Discussionmentioning

confidence: 99%

Separation and Quantitation of Phospholipids and Lysophospholipids by High-Performance Liquid Chromatography

Lesnefsky

Stoll

Minkler

et al. 2000

Analytical Biochemistry

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Section: Discussionmentioning

confidence: 99%

Separation and Quantitation of Phospholipids and Lysophospholipids by High-Performance Liquid Chromatography

Lesnefsky

Stoll

Minkler

et al. 2000

Analytical Biochemistry

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“…The major portion (75-90%) of CL in the IMM is located on the matrix side (Daum, 1985). CL is 9.2 mol% of total IMM lipids (Gomez and Robinson, 1999). Since most CL is located on the matrix side, the local concentration of CL could be as high as ~20 mol% CL, but because CL can be found associated with proteins and is also transferred across the membrane, its concentration will vary based on location and state of the cell.…”

Section: Introductionmentioning

confidence: 99%

X-ray structure, thermodynamics, elastic properties and MD simulations of cardiolipin/dimyristoylphosphatidylcholine mixed membranes

Boscia

Treece

Mohammadyani

et al. 2014

Chemistry and Physics of Lipids

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Cardiolipins (CLs) are important biologically for their unique role in biomembranes that couple phosphorylation and electron transport like bacterial plasma membranes, chromatophores, chloroplasts and mitochondria. CLs are often tightly coupled to proteins involved in oxidative phosphorylation. The first step in understanding the interaction of CL with proteins is to obtain the pure CL structure, and the structure of mixtures of CL with other lipids. In this work we use a variety of techniques to characterize the fluid phase structure, material properties and thermodynamics of mixtures of dimyristoylphosphatidylcholine (DMPC) with tetramyristoylcardiolipin (TMCL), both with 14-carbon chains, at several mole percentages. X-ray diffuse scattering was used to determine structure, including bilayer thickness and area/lipid, the bending modulus, KC, and Sxray, a measure of chain orientational order. Our results reveal that TMCL thickens DMPC bilayers at all mole percentages, with a total increase of ~6 Å in pure TMCL, and increases AL from 64 Å2 (DMPC at 35°C) to 109 Å2 (TMCL at 50°C). KC increases by ~50%, indicating that TMCL stiffens DMPC membranes. TMCL also orders DMPC chains by a factor of ~2 for pure TMCL. Coarse grain molecular dynamics simulations confirm the experimental thickening of 2 Å for 20 mol% TMCL and locate the TMCL headgroups near the glycerol-carbonyl region of DMPC; i.e., they are sequestered below the DMPC phosphocholine headgroup. Our results suggest that TMCL plays a role similar to cholesterol in that it thickens and stiffens DMPC membranes, orders chains, and is positioned under the umbrella of the PC headgroup. CL may be necessary for hydrophobic matching to inner mitochondrial membrane proteins. Differential scanning calorimetry, Sxray and CGMD simulations all suggest that TMCL does not form domains within the DMPC bilayers. We also determined the gel phase structure of TMCL, which surprisingly displays diffuse X-ray scattering, like a fluid phase lipid. AL = 40.8 Å2 for the ½TMCL gel phase, smaller than the DMPC gel phase with AL = 47.2 Å2, but similar to AL of DLPE = 41 Å2, consistent with untilted chains in gel phase TMCL.

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“…The second silicic acid HPLC procedure was performed using an isocratic normal-phase HPLC with cyclohexane:isopropanol:5 mM phosphoric acid in water 50:50:2.9, v/v/v as a mobile phase [11]. This method is designed to resolve cardiolipin without interference from other phospholipids.…”

mentioning

confidence: 99%

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of cardiolipin extracted from detergent-solubilized mitochondrial electron transfer complexes

McDonald-Marsh¹,

Carroll²,

Robinson³

et al. 2006

Analytical Biochemistry

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Quantitative Determination of Cardiolipin in Mitochondrial Electron Transferring Complexes by Silicic Acid High-Performance Liquid Chromatography

Cited by 36 publications

References 28 publications

Separation and Quantitation of Phospholipids and Lysophospholipids by High-Performance Liquid Chromatography

Separation and Quantitation of Phospholipids and Lysophospholipids by High-Performance Liquid Chromatography

X-ray structure, thermodynamics, elastic properties and MD simulations of cardiolipin/dimyristoylphosphatidylcholine mixed membranes

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of cardiolipin extracted from detergent-solubilized mitochondrial electron transfer complexes

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