Quantitative determination of carcinogenic mycotoxins in human and animal biological matrices and animal-derived foods using multi-mycotoxin and analyte-specific high performance liquid chromatography-tandem mass spectrometric methods

Cao, Xiaoqin; Li, Xiaofei; Li, Jian; Niu, Yunhui; Shi, Lu; Fang, Zhiwei; Zhang, Tao; Ding, Hong

doi:10.1016/j.jchromb.2017.10.006

Cited by 57 publications

(65 citation statements)

References 17 publications

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“…These results are better or similar to the class-specific methods used for the direct measurement of aflatoxins in plasma or serum. For instance, reported LLOQs for aflatoxins using LC-MS/MS analysis ranged from 1 ng/mL for ethyl acetate LLE [57], 0.21-0.43 ng/mL for dilute-and-shoot method by Cao et al [58], and 0.13-0.42 ng/mL for HLB SPE [59]. It should be noted that all three of these studies used S/N ratio for LLOQ determination, while our study used the more stringent accuracy and precision requirements.…”

Section: Results Of Methods Validationmentioning

confidence: 99%

Liquid chromatography – high resolution mass spectrometry method for monitoring of 17 mycotoxins in human plasma for exposure studies

Slobodchikova

Vuckovic

2018

Journal of Chromatography A

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Mycotoxins are secondary metabolites produced by filamentous fungi. Primary route of human exposure to mycotoxins is the intake of the contaminated food. Minimizing mycotoxin exposure is important for population health, as their chronic toxic effects have been associated with kidney and liver diseases, some types of cancer and immunosuppression. The objective of this work was to develop and validate a multi-class mycotoxin method suitable for exposure monitoring of mycotoxins in human plasma. A sensitive liquid chromatography - mass spectrometry method was developed for 17 mycotoxins: nivalenol (NIV), deoxynivalenol, fusarenon X, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, T-2 toxin, HT-2 toxin, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, zearalenone, α-zearalenol (α-ZOL), β-zearalenol, zearalanone, α-zeranol and, and β-zeranol. The method relies on three-step liquid-liquid extraction with ethyl acetate to eliminate the need for immunoaffinity extraction and minimize ionization matrix effects. Chromatographic separation of mycotoxins, including all isomers, was achieved with pentafluorophenyl column and water/methanol mobile phase. Mycotoxin detection and quantitation were performed using high-resolution mass spectrometry on LTQ Velos Orbitrap, in both positive and negative electrospray ionization (ESI(+) and (ESI(-)). The use of 0.02% acetic acid as mobile phase additive for ESI(-) resulted in significant increase in ionization efficiency ranging from 1.7 to 26 times for mycotoxins that ionize better in ESI(-). The optimized method was validated according to FDA guidance procedures. LOQs of all mycotoxins ranged from 0.1 to 0.5 ng/ml, except NIV which resulted in LOQ of 3 ng/ml because of low extraction recovery of this highly polar mycotoxin. Mean intra-day accuracy ranged from 85.8% to 116.4%, and intra-day precision (n = 6) ranged from 1.6% to 12.5% RSD for all mycotoxins except α-ZOL where mean accuracy ranged from 72.9% to 97.2%. Inter-day accuracy and precision were 85.6%-111.5% and 2.7-15.6% RSD respectively, showing good analytical performance of the method for biomonitoring.

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Section: Results Of Methods Validationmentioning

confidence: 99%

Liquid chromatography – high resolution mass spectrometry method for monitoring of 17 mycotoxins in human plasma for exposure studies

Slobodchikova

Vuckovic

2018

Journal of Chromatography A

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“…Their occurrence and concentrations vary considerably among foods due to factors such as crop susceptibility, climate change, storage and transportation conditions, as well as sanitary standards [3,4]. Mycotoxins are hepatotoxic, nephrotoxic, teratogenic, carcinogenic, cytotoxic, immunosuppressive, inflammatory, neurotoxic, and estrogenic, posing diverse health hazards to humans and animals [5][6][7][8]. In particular, fumonisins (FBs) and ochratoxin a (OTA) have been classified as possible human carcinogens (Group 2B).…”

Section: Introductionmentioning

confidence: 99%

“…Among the published methods for mycotoxin determination in biological samples, including HPLC [20,31,32], GC-MS/MS [33], LC-MS [34,35], LC-MS/MS [4,18,36,37], and LC-HRMS [38], LC-MS/MS provides remarkable selectivity, accuracy and sensitivity. Most of the methods were applied to detect common regulated mycotoxins [4,5,8,19,32,37,[39][40][41][42], employing various sample preparation strategies such as the "dilute and shoot" approach [4,5,37,41], QuEChERS [15], liquid-liquid extraction (LLE) [20], immunoaffinity (IAC) columns [41,42], solid phase extraction (SPE) [18], and various combinations of these techniques [13]. However, only a few methods targeted for Alternaria and emerging Fusarium mycotoxins, including ENNB [38,43], AOH [3], AOH and AME [15], TeA [44], ENNs, and BEA [7], for human biomonitoring.…”

Section: Introductionmentioning

confidence: 99%

Development of a Sensitive and Reliable UHPLC-MS/MS Method for the Determination of Multiple Urinary Biomarkers of Mycotoxin Exposure

Liu

Zhao

et al. 2020

Toxins

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A variety of mycotoxins from different sources frequently contaminate farm products, presenting a potential toxicological concern for animals and human. Mycotoxin exposure has been the focus of attention for governments around the world. To date, biomarkers are used to monitor mycotoxin exposure and promote new understanding of their role in chronic diseases. The goal of this research was to develop and validate a sensitive UHPLC-MS/MS method using isotopically-labeled internal standards suitable for accurate determination of 18 mycotoxin biomarkers, including fumonisins, ochratoxins, Alternaria and emerging Fusarium mycotoxins (fumonisin B1, B2, and B3, hydrolyzed fumonisin B1 and B2, ochratoxin A, B, and alpha, alternariol, alternariol monomethyl ether, altenuene, tentoxin, tenuazonic acid, beauvericin, enniatin A, A1, B, and B1) in human urine. After enzymatic digestion with β-glucuronidase, human urine samples were cleaned up using HLB solid phase extraction cartridges prior to instrument analysis. The multi-mycotoxin and analyte-specific method was validated in-house, providing satisfactory results. The method provided good linearity in the tested concentration range (from LOQ up to 25–500 ng/mL for different analytes), with R2 from 0.997 to 0.999. The limits of quantitation varied from 0.0002 to 0.5 ng/mL for all analytes in urine. The recoveries for spiked samples were between 74.0% and 133%, with intra-day precision of 0.5%–8.7% and inter-day precision of 2.4%–13.4%. This method was applied to 60 urine samples collected from healthy volunteers in Beijing, and 10 biomarkers were found. At least one biomarker was found in all but one of the samples. The high sensitivity and accuracy of this method make it practical for human biomonitoring and mycotoxin exposure assessment.

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“…No entanto, a co-ocorrência de AFLs e FBs foi encontrada em duas amostras de pacientes com carcinoma hepatocelular. É possível destacar, de acordo com os dados dos autores, que os valores das micotoxinas carcinogênicas em pacientes com carcinoma hepatocelular foram significativamente maiores do que em pacientes controles (Cao et al, 2018).…”

Section: Ocorrência De Micotoxinas Em Amostras Clínicasunclassified

“…Research, Society and Development, v. 9, n. 4, e24942482, 2020(CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i4.2482 5 Com o alto consumo de cereais contaminados com micotoxinas, estes são possivelmente metabolizados no organismo e eficazmente excretados. Por isso, quantidades quantificáveis destas toxinas são esperadas em amostras clínicas como sangue e urina (Brewer, Thrasher, Straus, Madison, & Hooper, 2013;Cao et al, 2018;Ezekiel et al, 2014).…”

Section: Introductionunclassified

Micotoxinas: riscos à saúde humana pela ingestão diária de alimentos contaminados e sua ocorrência em amostras clínicas

Savi

Zenaide

2020

RSD

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1 Micotoxinas: riscos à saúde humana pela ingestão diária de alimentos contaminados e sua ocorrência em amostras clínicas Mycotoxins: risks to human health due to daily ingestion of contaminated food and its occurrence in clinical samples Micotoxinas: riesgos para la salud humana por la ingestión diaria de alimentos contaminados y su ocurrencia en muestras clínicas Resumo Fungos toxigênicos podem produzir micotoxinas em alimentos quando em condições favoráveis de temperatura e umidade. A ingestão diária destas micotoxinas nos alimentos pode trazer prejuízos a saúde humana e animal. O objetivo deste estudo foi verificar a exposição do organismo humano as micotoxinas e a sua ocorrência em amostras clínicas.Nesta revisão bibliográfica, os artigos foram selecionados por meio de banco de dados usando palavras combinadas para a busca do tema principal, sendo recrutados 60 trabalhos para a revisão geral e selecionados 20 desses para os dados de exposição às micotoxinas e achados em amostras clínicas. As micotoxinas são encontradas em diversos alimentos, desde cereais, leguminosas, frutas e vegetais. São moléculas altamente resistentes que permanecem mesmo após processamento na indústria, inclusive nos seus subprodutos. A intoxicação crônica pode ocorrer nos indivíduos, trazendo prejuízos a saúde. O achado de micotoxinas nas amostras clínicas de indivíduos de uma determinada região pode auxiliar no conhecimento de quanto esta população está realmente exposta, possibilitando assim, diferentes providências com o intuito de evitar possíveis doenças. A avaliação da exposição às micotoxinas é de fundamental importância para a saúde pública, uma vez que pode auxiliar na prevenção ou redução de doenças ocasionadas por estas toxinas. Esta revisão pode contribuir para instigar novas Research, Society and Development, v. 9, n. 4, e24942482, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i4.2482 2 pesquisas de investigação sobre a possível relação da exposição pela ingestão de micotoxinas com as doenças decorrentes da sua interação com o organismo. Palavras-chave:Micotoxinas; Alimentos; Exposição; Saúde; Urina. AbstractToxigenic fungi can produce mycotoxins in food in favorable conditions of temperature and humidity. The daily intake of mycotoxins in food can cause harm to human and animal health.The aim of this study was to verify the exposure of the human organism to mycotoxins and their occurrence in clinical samples. The current study evaluated the risks to human health due to the toxicity caused by mycotoxins when consumed daily in foods, as well as their occurrence in clinical samples. In this bibliographic revision, the articles were selected through a database using combined words to search of main subject, which were recruited 60 papers for the general revision and 20 papers for the data of exposure mycotoxins by the daily intake and mycotoxins in clinical samples. Mycotoxins are often found in foods, including cereals, pulses, fruits and vegetables. They are highly resistant molecules that rem...

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Quantitative determination of carcinogenic mycotoxins in human and animal biological matrices and animal-derived foods using multi-mycotoxin and analyte-specific high performance liquid chromatography-tandem mass spectrometric methods

Cited by 57 publications

References 17 publications

Liquid chromatography – high resolution mass spectrometry method for monitoring of 17 mycotoxins in human plasma for exposure studies

Liquid chromatography – high resolution mass spectrometry method for monitoring of 17 mycotoxins in human plasma for exposure studies

Development of a Sensitive and Reliable UHPLC-MS/MS Method for the Determination of Multiple Urinary Biomarkers of Mycotoxin Exposure

Micotoxinas: riscos à saúde humana pela ingestão diária de alimentos contaminados e sua ocorrência em amostras clínicas

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