Quantitative Determination of Cannabinoids in Individual Glandular Trichomes of Cannabis Sativa L. (Cannabaceae)

Abstract

Cannabinoid levels of individual mature glandular trichomes from two clones and two strains of Cannabis sativa L., which included both drug and fiber phenotypes, were investigated by gas‐liquid chromatographic analyses. Capitate‐stalked glands were selectively harvested from vein and nonvein areas of pistillate bracts while capitate‐sessile glands were harvested from these areas of leaves. The qualitative cannabinoid profile characteristic of the strain or clone was maintained in the individual capitate‐stalked glands while the quantitative cannabinoid profiles varied with each strain or clone and between vein and nonvein areas as well. Capitate‐sessile glands were found to contain conspicuously lower levels of cannabinoids than capitate‐stalked glands. This study emphasizes that glands of Cannabis represent a dynamic system within the cannabinoid synthesizing activities of this plant.

Separation of aroma compounds from industrial hemp inflorescences (Cannabis sativa L.) by supercritical CO2 extraction and on-line fractionation

“…Inflorescences of fiber type Cannabis sativa L. cultivars are generally considered waste parts for fiber industry, although the inflorescences' volatiles are pleasant to the human sensory system and could be used as flavorings for beverages (food industry) or ingredients for body care products (cosmetic industry). Cannabis scent does not originate from the terpenophenolic cannabinoids, produced by glandular trichomes that occur on most aerial surfaces of the plant (Dayanandan and Kaufman, 1976;Turner et al, 1978), but from the more volatile monoterpenes and sesquiterpenes (Turner et al, 1980). Traditionally, the recovery of floral fragrances from plants is by hydrodistillation or steam distillation to produce essential oils.…”

Intérêt du rapport Δ-9-THC / CBD dans le contrôle des cultures de chanvre industriel

“…Le rapport a = A-9-THC / CBD n'a jamais dépassé 0,105. Des expériences similaires, menées sur des chanvres de diverses origines, montrent également bien cette très importante différen-ce selon la partie prélevée (36,37).…”

Biosynthesis of cannabinoids

“…The phenolic moiety is generated by a polyketide-type reaction sequence. The data support geranyl diphosphate and the polyketide, olivetolic acid, as specific intermediates in the biosynthesis of cannabinoids.Keywords: biosynthesis; cannabinoid; deoxyxylulose; NMR spectroscopy; polyketide.Cannabinoids, a group of terpenophenolics, are accumulated in considerable amounts in glandular trichomes of Cannabis sativa (Cannabaceae) [1]. Numerous representatives of this group have been characterized.…”

“…Cannabinoids, a group of terpenophenolics, are accumulated in considerable amounts in glandular trichomes of Cannabis sativa (Cannabaceae) [1]. Numerous representatives of this group have been characterized.…”

“…In order to analyse the biosynthetic origin of cannabinoid precursors, cut sprouts of C. sativa were proffered with a mixture of unlabeled glucose and [U- 13 C 6 ]glucose or [1][2][3][4][5][6][7][8][9][10][11][12][13] C]glucose. Tetrahydrocannabinolic acid (7) and cannabichromenic acid (6) were isolated and analyzed by quantitative NMR spectroscopy.…”