Quantitative determination of calcium, magnesium, and zinc in fingernails by laser-induced breakdown spectroscopy

Rusak, David A.; Zeleniak, Ann E.; Obuhosky, Jillian L.; Holdren, Scott; Noldy, Craig A.

doi:10.1016/j.talanta.2013.08.022

Cited by 25 publications

(7 citation statements)

References 15 publications

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“…This behavior may also explain the observation by Rusak et al. 31 that pressed keratin pellets were not an effective LIBS calibration standard for an LIBS fingernail assay using a 1064 nm laser. Pressed keratin pellets created from a uniform homogenous powder would have no such asymmetrical striations or vertical layering, yielding more reproducible results than ablation, but not effectively modeling the behavior of a true fingernail.…”

Section: Resultsmentioning

confidence: 91%

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Determination of the Zinc Concentration in Human Fingernails Using Laser-Induced Breakdown Spectroscopy

2017

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The absolute concentration of Zn in human fingernail clippings was determined ex vivo using 1064 nm laser-induced breakdown spectroscopy and confirmed by speciated isotope dilution mass spectrometry. A nail testing protocol that sampled across the nail (perpendicular to the direction of growth) was developed and validated by scanning electron microscopy energy-dispersive X-ray spectrometry. Using this protocol, a partial least squares (PLS) regression model predicted the Zn concentration in the fingernails of five people to within an average of 7 ppm. The variation in the Zn concentration with depth into the nail determined by laser-induced breakdown spectroscopy was studied and showed no systematic variation for up to 15 subsequent laser pulses in one location. The effects of nail hydration (dehydrated and over-hydrated) and nail surface roughness were investigated to explain an anomalously large scatter observed in the measurements. This scatter was attributed to the layered nature and fibrous structure of the fingernails, which resulted in non-uniform ablation as determined by scanning electron microscopy. This work demonstrates that a protocol consisting of low pulse energy (<10 mJ) 1064 nm laser pulses incident on human fingernail clippings in an Ar environment can produce quantifiable Zn emission in the laser-induced plasma and that the measured Zn intensity can be used to accurately predict the Zn concentration in human fingernails.

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Section: Resultsmentioning

confidence: 91%

“…Rusak et al. 31 utilized LIBS with a 1064 nm laser to study Mg, Ca, and Zn in human fingernails obtained from 11 volunteers. Utilizing the Zn I line at 481 nm, a calibration graph with an R 2 value of 0.9896 was created after the determination of the absolute Zn concentration via atomic absorption spectrometry.…”

Section: Introductionmentioning

confidence: 99%

Determination of the Zinc Concentration in Human Fingernails Using Laser-Induced Breakdown Spectroscopy

2017

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“…148 As aplicações biológicas em LIBS são principalmente de natureza qualitativa, mas também ocorrem análises quantitativas. A aplicação da bio-LIBS estende-se a uma ampla variedade de amostras como, sangue, 158 cabelo, 238 unhas, 239 fígado de pacientes com mal de Wilson, 240 urina, 241 e dentes. 242 O grande potencial da LIBS nesse campo é que não é necessário nenhum conhecimento prévio sobre as sequências de ácido nucléico ou anticorpos contra antígenos bacterianos conhecidos, em contraste com as técnicas de diagnóstico clínico estabelecidas.…”

Section: Biológicas E Farmacêuticasunclassified

Laser Induced-Breakdown Spectroscopy (Libs): Histórico, Fundamentos, Aplicações E Potencialidades

Costa¹,

Augusto²,

Castro³

et al. 2019

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This review attempts to present a broad view of the technique laser-induced breakdown spectroscopy (LIBS), since its origin, fundamentals, sample preparation strategies, use of chemometric tools for data processing, the advantages and limitations with regard to qualitative and quantitative analysis, aplications and future trends as an analytical technique that is in consolidation in analytical chemistry. LIBS is a rapid and versatile, which is able of providing qualitative and quantitative analytical information for practically any sample, in a virtually non-destructive way, without any substantial sample preparation. The instrumentation is simple, robust, compact, and enables remote analysis. The advancement of the LIBS applications in the areas of agriculture, environmental, food, biological/pharmaceutical, forensic, metallurgy and electronic waste were discussed in detail in this review. We consider that this review will be an important tool concerning dissemination of the LIBS, mainly for audience of undergraduate courses, and disciplines related to instrumental analysis.

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“…Good correlation was obtained between the predicted values and reference values. Rusak et al performed the quantitative determination of Ca, Mg, and Zn in 11 fingernails by LIBS using calibration curves, and the best agreement was obtained for the determination of Zn. Sturm et al used LIBS for inline analysis of liquid slag at a steelworks, and the major components such as CaO, Fe, SiO 2 , MgO, Mn, and Al 2 O 3 were compared with reference values from the steelworks' laboratory for solid pressed slag samples as well as for samples from the liquid slag.…”

Section: Quantitative Analysismentioning

confidence: 99%

Chemometrics in laser‐induced breakdown spectroscopy

Zhang

Tang

2018

Journal of Chemometrics

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Laser-induced breakdown spectroscopy (LIBS) is a new type of elemental analytical technology with the advantages of real-time, online, and noncontact as well as enabling the simultaneous analysis of multiple elements. It has become a frontier analytical technique in spectral analysis. However, the issue of how to improve the accuracy of qualitative and quantitative analyses by extracting useful information from a large amount of complex LIBS data remains the main problem for the LIBS technique. Chemometrics is a chemical subdiscipline of multi-interdisciplinary methods; it offers advantages in data processing, signal analysis, and pattern recognition. It can solve some complicated problems that are difficult for traditional chemical methods. In this paper, we reviewed the research progress of chemometrics methods in LIBS for spectral data preprocessing as well as for qualitative and quantitative analyses in the most recent 5 years (2012-2016). KEYWORDSchemometrics, data preprocessing, laser-induced breakdown spectroscopy, qualitative analysis, quantitative analysis | INTRODUCTIONAs one of the most promising analytical tools in the 21st century, laser-induced breakdown spectroscopy (LIBS) is a novel analytical technique for materials and elements; it is based on atomic emission spectroscopy and a laser as an excitation source. 1-4 In LIBS, a low-energy pulse laser (typically tens to hundreds of millijoules per pulse) generated from a laser device is reflected by a plane mirror and focused by a plano-convex lens onto the sample surface; a laser-induced plasma with high temperature is then generated. The light with different frequencies is radiated during the plasma cooling process, and it is collected by an optical fiber coupled to a spectrometer. The qualitative and quantitative analyses by the LIBS technique can be performed using the wavelength and integrated intensity of the spectral line. A schematic diagram of the LIBS instrument is presented in Figure 1. It consists of Nd:YAG laser, optical part, X-Y-Z manual micrometric stage, optical fiber, spectrometer, Charge coupled device (CCD) camera, and computer.The LIBS technology has benefited from the invention of the ruby crystal laser 5 and was first reported by Brech and Cross. 6 It has become a research focus in the spectral field with the development of laser and optical detection techniques. Compared with conventional analytical tools, LIBS demonstrates some substantial advantages with regards to being rapid, real-time, on-site, and nondestructive while allowing remote detection without complicated sample preparation procedures as well as simultaneous multicomponent analysis. 7-9 Because of these advantages, LIBS technology has been widely used in process analysis and in the control of industrial manufacturing, food supervision, environmental monitoring, historical relic identification, and space exploration. [10][11][12][13] The National Aeronautics and

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Quantitative determination of calcium, magnesium, and zinc in fingernails by laser-induced breakdown spectroscopy

Cited by 25 publications

References 15 publications

Determination of the Zinc Concentration in Human Fingernails Using Laser-Induced Breakdown Spectroscopy

Determination of the Zinc Concentration in Human Fingernails Using Laser-Induced Breakdown Spectroscopy

Laser Induced-Breakdown Spectroscopy (Libs): Histórico, Fundamentos, Aplicações E Potencialidades

Chemometrics in laser‐induced breakdown spectroscopy

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