Quantitative determination of alginic acid in pharmaceutical formulations using capillary electrophoresis

Moore, Douglas E.; Miao, William Gang; Benikos, Con

doi:10.1016/j.japna.2003.08.005

Cited by 12 publications

(5 citation statements)

References 9 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alginic acid (Fig. 3b), is a gelling polysaccharide contains 1, 4-linked b-D-mannuronic and a-L-guluronic acid residues arranged in a non-regular, blockwise order along the chain (Andrade et al 2004;Moore et al 2004). It is also called algin or alginate, and this naturally occurring hydrophilic colloidal polysaccharide distributed widely in the cell walls and intracellular material of the marine brown seaweeds.…”

Section: Bioactive Compounds From Brown Seaweeds With Various Functiomentioning

confidence: 99%

Biological activities and potential cosmeceutical applications of bioactive components from brown seaweeds: a review

2011

View full text Add to dashboard Cite

Seaweeds are the primary producers of all aquatic ecosystems. Chemical constituents isolated from diverse classes of seaweeds exert a wide range of nutritional, functional and biological activities. Unique metabolites of seaweeds possess specific biological properties that make them potential ingredients of many industrial applications such as functional foods, pharmaceuticals and cosmeceuticals. Cosmeceuticals of natural origin are becoming more popular than synthetic cosmetics. Hence, the investigation of new seaweeds derived functional components, a different source of natural products, has proven to be a promising area of cosmeceutical studies. Brown seaweeds also produce a range of active components including unique secondary metabolites such as phlorotannins and many of which have specific biological activities that give possibilities for their economic utilization. Brown seaweeds derived active compounds have been shown various functional properties including, antioxidant, antiwrinkling, whitening, antiinflammatory and antiallergy.It is well-known that these kind of biological effects are closely associated with cosmeceutical preparations. This communication reviews the current knowledge on brown seaweeds derived metabolites with various biological activities and the potential use as cosmeceutical ingredients. It is hoped that the reviewed literature on multifunctional properties of brown seaweeds will improve access to the seaweed based natural products specially the ability to incorporate these functional properties in cosmeceutical applications.

show abstract

Section: Bioactive Compounds From Brown Seaweeds With Various Functiomentioning

confidence: 99%

Biological activities and potential cosmeceutical applications of bioactive components from brown seaweeds: a review

2011

View full text Add to dashboard Cite

show abstract

“…Excipients such as SDS [106] or arginic acid [107] can be analysed as raw materials or when present within formulations. For example, SDS levels were determined [106] within various tablet batches.…”

Section: Analysis Of Small Molecules and Ionsmentioning

confidence: 99%

Capillary electrophoresis for the analysis of small‐molecule pharmaceuticals

2006

View full text Add to dashboard Cite

This paper reviews the application of CE to the analysis of small-molecule pharmaceuticals. The areas of pharmaceutical analysis covered are enantiomer separation, the analysis of small molecules such as amino acids or drug counter-ions, pharmaceutical assay, determination of related substances and physicochemical measurements such as log P and pK(a) of compounds. The different electrophoretic modes available and their advantages for pharmaceutical analysis are described. Recent applications of CE for each subject area are tabulated with electrolyte details.

show abstract

“…AA is present in the cell walls and intracellular material of the marine brown algae (belonging to the Phaeophyceae) and some bacteria [1] AA, like other natural polysaccharides, is polydisperse with respect to molecular mass, but the number of monosaccharide units and their distribution in the tissue extracts may range from 1000 to 10 000 depending on the harvest conditions and the extraction and purification procedures [2][3][4]. Owing to its gelling, stiffening and stabilizing properties, AA is used as an ingredient in food and a number of pharmaceutical applications such as in antacid formulations for the treatment of gastroesophageal reflux [5,6]. Furthermore, microspheres have been produced encapsulating liposomes coated with AA and poly-L-lysine membranes as controlled-release systems for liposome-associated macromolecules [7].…”

Section: Introductionmentioning

confidence: 99%

“…However, these analytical approaches were not applicable to quantitative determination of the total AA content. Moore et al [6] recently reported a CE/UV determination of AA in antacid formulations. However, this method used time-consuming pre-treatment steps with methanol/acid to remove interfering ingredients in the formulations.…”

Section: Introductionmentioning

confidence: 99%

Micellar electrokinetic capillary chromatography determination of alginic acid in pharmaceutical formulations after treatment with alginate lyase and UV detection

Volpi

2008

Electrophoresis

View full text Add to dashboard Cite

A new highly specific and sensitive capillary electrophoresis method (electrokinetic chromatography with SDS) for the determination of the total alginic acid (AA) content in pharmaceutical formulations is described by means of capillary electrophoresis at 230 nm after treatment with alginate lyase [4.2.2.3] and separation of unsaturated products, Delta-oligomers (DeltaHexA-[HexA](n)), in particular, DP3 (DeltaHexA-HexA-HexA) and DP4 (DeltaHexA-HexA-HexA-HexA). Using a buffer constituted with 10 mM sodium borate and 50 mM SDS at pH 9.0, micellar electrokinetic capillary chromatography was able to determine with very high resolution the AA Delta-oligomers produced by the action of the lyase (mainly DP3 and DP4) as one single species. The intra- and inter-day variations (CV%) were between 6.3 and 9.1 for migration time and between 2.5 and 5.7 for peak area, respectively. The calibration curve showed good linearity for the examined concentration range (60-360 ng) with an average correlation coefficient greater than 0.980. The lowest detection limit and the lowest quantitation limit of the method were 15 ng (0.25 mg/mL) and 40 ng (0.67 mg/mL), respectively. The intra- and inter-day variations in terms of CV% were 5.5 and 8.6%, respectively, and the intra- and inter-day accuracy was estimated to range from 4.1 to 8.9%, while the percent recoveries of AA were calculated to be 102, 97 and 93% for different AA amounts. Variations in temperatures, voltage and buffer composition in comparison with adopted conditions within a 10% limit do not modify the electrophoresis results. The evaluation of AA was performed in both solid and liquid pharmaceutical formulations also in the presence of other ingredients, in particular, aluminium, sodium and potassium bicarbonate, and emulsifying and flavouring agents. The quantitative results obtained were 101.2+/-3.4% of AA content in tablets and 98.4+/-2.8% in liquid formulation, in total conformity with the label claims.

show abstract

Quantitative determination of alginic acid in pharmaceutical formulations using capillary electrophoresis

Cited by 12 publications

References 9 publications

Biological activities and potential cosmeceutical applications of bioactive components from brown seaweeds: a review

Biological activities and potential cosmeceutical applications of bioactive components from brown seaweeds: a review

Capillary electrophoresis for the analysis of small‐molecule pharmaceuticals

Micellar electrokinetic capillary chromatography determination of alginic acid in pharmaceutical formulations after treatment with alginate lyase and UV detection

Contact Info

Product

Resources

About