Quantitative determination of acemetacin and its metabolite indometacin in blood and plasma by column liquid chromatography

Schöllnhammer, G; Dell, H. D.; Doersing, K.; Kamp, R

doi:10.1016/s0378-4347(00)83725-1

Cited by 11 publications

(4 citation statements)

References 7 publications

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“…Another acemetacin metabolite is p-chlorobenzoic acid (PCBA), which is a minor metabolite. [4,5] However, in our laboratory, these HPLC methods showed insufficient specificity and extraction to efficiently analyze ACE, IND, and internal standard (IS) simultaneously in plasma. [3 -5] A TLC method has the disadvantages of laborious and time-consuming steps, as well as high detection limits of 50 mg/mL.…”

Section: Hplc Methods For the Pharmacokinetics Study Of Acemetacin In mentioning

confidence: 99%

HPLC Method for the Pharmacokinetics Study of Acemetacin in Human Plasma

Ban

Cho

Jang

et al. 2005

Journal of Liquid Chromatography & Related Technologies

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A reproducible and specific high performance liquid chromatography (HPLC) method was developed to quantity plasma levels of acemetacin and its metabolite, indometacin in order to conduct a pharmacokinetics study. Plasma samples were prepared by liquid-liquid extraction using ethyl acetate. Extracted samples were analyzed on a reverse-phase C 18 column with a mobile phase consisting of 20 mM phosphate buffer (pH 2.9) and acetonitrile (60:40, v/v) and UV detection at 254 nm. Using this method, the pharmacokinetics study was carried out on eight volunteers after a single oral administration.

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Section: Hplc Methods For the Pharmacokinetics Study Of Acemetacin In mentioning

confidence: 99%

HPLC Method for the Pharmacokinetics Study of Acemetacin in Human Plasma

Ban

Cho

Jang

et al. 2005

Journal of Liquid Chromatography & Related Technologies

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“…However, ACM can be successfully used in patients at risk of cardiovascular or gastrointestinal disorders. Until now, several methods have been reported to determine ACM in different biological matrixes, including column liquid chromatography [9], high‐performance liquid chromatography (HPLC) [8], micellar liquid chromatography [10], micellar electrokinetic capillary chromatography [11], capillary zone electrophoresis [12], and capillary liquid chromatography and mass spectrometry [13]. In this article, we describe a method developed by us which is more sensitive than most of the methods presented in the literature.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Determination of an Anti‐rheumatoid Drug Acemetacin on Graphite Flake Paste Electrode and Glassy Carbon Electrode

et al. 2020

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In this study, a simple and sensitive square wave voltammetric procedure has been developed for the determination of acemetacin (ACM) at graphite flake paste electrode (GFPE) and glassy carbon electrode (GCE). Under optimized conditions, the dependence of ACM peak current on its concentration showed wide linear range: 0.03–1.0 μmol L−1 and 0.7–15.0 μmol L−1 at GFPE and GCE, respectively. The developed method was successfully applied for the determination of ACM in pharmaceuticals and spiked urine with satisfying recoveries. The electrochemical oxidation of ACM is an irreversible process controlled by mixed nature of the mass transfer process.

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“…Looking over the published methods, it was found that ACM was determined by different analytical methods such as UV‐spectrophotometry and different HPLC methods either in pharmaceutical formulation or in plasma . ACM along with its metabolite (IMP‐B) were determined by two spectrophotometric methods , TLC with denisitometry and HPLC methods . Only two methods were found for the determination of the proposed drug and the impurities .…”

Section: Introductionmentioning

confidence: 99%

Design and optimization of a reversed‐phase HPLC with diode array detection method for the determination of acemetacin and its toxic impurities using experimental design

Abdelgawad

Owis

Abdelwahab

2018

Separation Science Plus

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A reversed‐phase high‐performance liquid chromatography with diode array detection method was developed with the aid of statistical approaches for the determination of acemetacin and its official impurities 4‐chlorobenzoic acid and indometacin. Two‐level full factorial design was used to optimize the used mobile phase where three chromatographic variables were studied, amount of acetonitrile, amount of H3PO4 acid, and mobile phase flow rate. The selected responses were analysis time and chromatographic resolution between adjacent peaks (peaks of indometacin and acemetacin). The impacts of the amount of acetonitrile and the mobile phase flow rate individually were significant on the analysis time (P = 0.0277 and 0.0424, respectively). Also the amount of acetonitrile had a meaningful effect on the resolution (P = 0.0085). Finally, chromatographic separation was performed on Zorbax® Eclipse C18 column using acetonitrile/water (containing 0.05% H3PO4; 55:45, v/v) at 1.5 mL/min as an optimized mobile phase using full factorial experimental design with UV detection at 230 nm. 4‐Chlorobenzoic acid appeared at 3.29 min, indometacin at 9.07 min and acemetacin at 10 min. The method fulfilled United States Pharmacopoeia validation standards. In addition, system suitability parameters were calculated, and the results were within acceptable criteria. Commercially available tablets were analyzed, and good recoveries were obtained. Statistical comparison of the method with the reported one showed no significant difference.

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Quantitative determination of acemetacin and its metabolite indometacin in blood and plasma by column liquid chromatography

Cited by 11 publications

References 7 publications

HPLC Method for the Pharmacokinetics Study of Acemetacin in Human Plasma

HPLC Method for the Pharmacokinetics Study of Acemetacin in Human Plasma

Voltammetric Determination of an Anti‐rheumatoid Drug Acemetacin on Graphite Flake Paste Electrode and Glassy Carbon Electrode

Design and optimization of a reversed‐phase HPLC with diode array detection method for the determination of acemetacin and its toxic impurities using experimental design

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