Quantitative determination of aceclofenac and its three major metabolites in rat plasma by HPLC‐MS/MS

Abstract

We developed a method for the simultaneous quantification of aceclofenac and its three major metabolites in rat plasma. After protein precipitation with acetonitrile including flufenamic acid as an internal standard (IS), aceclofenac, diclofenac, 4'-hydroxyaceclofenac, 4'-hydroxydiclofenac, and the IS were chromatographed on a reverse-phase C18 analytical column. The isocratic mobile phase of acetonitrile/0.1% formic acid (aq; 9:1 [v/v]) was eluted at 0.3 mL/min. Quantification was performed on a triple-quadrupole mass spectrometer using electrospray ionization, and the ion transitions were monitored in selective reaction-monitoring mode. The coefficient of variation in the assay precision was less than 8%, and the accuracy was 92-103%. This method was successfully used to measure the concentrations of aceclofenac and its three major metabolites in rat plasma following the oral administration of a single 20 mg/kg oral dose of aceclofenac.

Simultaneous determination of 7‐O‐succinyl macrolactin A and its active major metabolite, macrolactin A in dog plasma using high‐performance liquid chromatography with UV detection

“…From these reports, nine candidate substances (ketoconazole, itraconazole, telmisartan, felodipine, gliclazide, metformin, roxithromycin, levofloxacin, naproxen) were selected and tested but were not successful as an IS. Specific nonsteroidal anti‐inflammatory drugs, e.g., aceclofenac, diclofenac, and flufenamic acid were examined based on our previous study , as well as naproxen . Based on these experiments, flufenamic acid could be used as an IS.…”

Quantitative determination of duloxetine and its metabolite in rat plasma by HPLC‐MS/MS

“…Stability under the experimental conditions was investigated at low and high QC concentrations. Short‐term, post‐extraction, freeze–thaw, and long‐term stabilities were assessed (Kim et al ., ). After an overnight fast, six Sprague–Dawley rats were given a single oral dose of 40 mg/kg of duloxetine.…”

Liquid chromatography–tandem MS/MS method for simultaneous quantification of paracetamol, chlorzoxazone and aceclofenac in human plasma: An application to a clinical pharmacokinetic study

“…ACL appears to be particularly well‐tolerated among the nonsteroidal anti‐inflammatory drugs with a lower incidence of gastrointestinal adverse effects (Sweetman, ). A literature survey revealed some methods for the determination of ACL in pharmaceutical dosage forms and biological fluids (El‐Bagary, Azzazy, ElKady, & Farouk, ; Hinz et al, ; Kang & Kim, ; Kim, Ahn, Noh, Kang, & Gwak, ).…”