Quantitative determination of 8-hydroxy-2′-deoxyguanosine in human urine by isotope dilution mass spectrometry: normal levels in hemochromatosis

Life Style and Urinary 8‐Hydroxydeoxyguanosine, a Marker of Oxidative DNA Damage: Effects of Exercise, Working Conditions, Meat Intake, Body Mass Index, and Smoking

“…It should be mentioned that the mean 8-OH-dG level of our analysis (4.12 µg/g creatinine) is similar to that determined by another group with the HPLC-ECD method (3.68-3.96 µg/g creatinine) 19) and to that measured by a GC-MS-method (3.33-3.95 µg/g creatinine) using an isotope dilution technique, 14) but is four times lower than that determined by an ELISA method (17.1 µg/g creatinine).…”

Body iron is a contributor to oxidative damage of DNA

“…A study by Holmberg and coworkers studied 8-OHdG excretion in hereditary hemochromatosis subjects, not supporting an association. However, in this study the subjects had been under treatment before the study and had only marginally elevated stored iron at the time of the study [21]. Instead, in a study by Kom and coworkers, increased urinary excretion of 8-isoprostaglandin -another marker of oxidative stress-was observed in hereditary hemochromatosis subjects.…”

Analysis of 8-hydroxy-2′-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry

“…Measurements of 8-OHdG in urine samples are especially well-suited to large-scale human studies and clinical applications because they are noninvasive [10,11]. Urinary 8-OHdG has been analyzed by several methods, such as enzyme-linked immunosorbent assay (ELISA) [12,13], high-performance liquid 4 chromatography with electrochemical detection (HPLC-ECD) [14], gas chromatography with mass spectrometry (GC/MS) [15,16], and liquid chromatography with tandem mass spectrometry (LC/MS/MS) [17][18][19][20][21][22][23][24][25][26]. The ELISA method suffers from the problem of non-selectivity because the antibody may cross-react with other substances present in urine [12,27,28].…”

“…The ELISA method suffers from the problem of non-selectivity because the antibody may cross-react with other substances present in urine [12,27,28]. HPLC-ECD has often been used [29], but it suffers from possible interference from the biological matrix, incompatibility of a stable isotope labeled internal standard [16,21]. For GC/MS analysis, 8-OHdG must be purified by HPLC and derivatized before analysis [15,16,28].…”

“…HPLC-ECD has often been used [29], but it suffers from possible interference from the biological matrix, incompatibility of a stable isotope labeled internal standard [16,21]. For GC/MS analysis, 8-OHdG must be purified by HPLC and derivatized before analysis [15,16,28].…”