Quantitative detection of secretory immunoglobulin A to Helicobacter pylori in gastric juice: Antibody capture enzyme‐linked immunosorbent assay

Hayashi, Shunji; Sugiyama, Toshiro; Hisano, Kazunari; Awakawa, Teruhito; Kurokawa, Ichiro; Yachi, Akira; Isogai, Hiroshi; Isogai, Emiko; Yokota, Kenji; Hirai, Yoshikazu; Oguma, Keiji; Fujii, Nobuhiro

doi:10.1002/(sici)1098-2825(1996)10:2<74::aid-jcla3>3.3.co;2-s

Cited by 4 publications

(7 citation statements)

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“…H. pylori infection induces class II major histocompatibility complex expression and CD4-positive T-cell activation (11); however, antigens associated with lymphocyte activation were not detected. We have reported that local immunoglobulin against hsp60 is associated with infiltrating cell numbers in the gastric mucosae of patients with gastritis (12) and have also reported that anti-hsp60 antibodies are relevant to gastric inflammation in patients with peptic ulcer during eradication therapy (25). In addition, the level of expression of CD40L on the peripheral blood mononuclear cells (PBMCs) of patients with MALT lymphoma was increased by cytokines (granulocyte-macrophage colony-stimulating factor and IL-4) or by cytokine plus hsp60 stimulation compared to the level of expression on PBMCs of patients with gastritis or healthy volunteers.…”

Section: Vol 8 2001supporting

confidence: 91%

Immunoglobulin G1 Antibody Response toHelicobacter pyloriHeat Shock Protein 60 Is Closely Associated with Low-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

Ishii

Yokota

Sugiyama

et al. 2001

Clin Diagn Lab Immunol

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Gastric mucosa-associated lymphoid tissue (MALT) lymphoma is related to Helicobacter pylori infection. Specifically, it has been pointed out that pathogenesis of MALT lymphoma involves the 60-kDa heat shock protein (hsp60). To investigate humoral immune responses to the H. pylori hsp60 in patients with gastroduodenal diseases and patients with MALT lymphoma, the hsp60 of H. pylori was expressed with a glutathione S-transferase fusion protein and was purified (recombinant hsp60). Sera were obtained from H. pylori-positive patients with gastroduodenal diseases (MALT lymphoma, n ‫؍‬ 13; gastric ulcer, n ‫؍‬ 20; duodenal ulcer, n ‫؍‬ 20; gastritis, n ‫؍‬ 20) and from H. pylori-negative healthy volunteers (n ‫؍‬ 9). Sera from patients with MALT lymphoma were also obtained at two times: before and after eradication therapy. Antibodies to hsp60 and H. pylori were assessed by enzyme-linked immunosorbent assay. The levels of immunoglobulin G (IgG) antibodies to the hsp60 of H. pylori-positive patients with gastroduodenal diseases were significantly elevated compared to those in the controls. The levels of IgG1 antibodies to hsp60 were elevated and correlated with the levels of anti-H. pylori antibodies in patients with MALT lymphoma. Humarol immunity against hsp60 may be important and relevant to gastroduodenal diseases induced by H. pylori infection.Helicobacter pylori is now recognized as a cause of gastritis, peptic ulcer, gastric cancer, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma (9, 19). Several H. pylori pathogenic factors, including CagA and VacA, may contribute to the gastric mucosal damage (6, 7). In recent years, it has also been accepted that host immune reactions play an important role in the pathogenesis of H. pylori infection. Although H. pylori is a noninvasive bacterium and is restricted to gastric epithelial cells, infection with H. pylori induces humoral and cellular immune responses in the gastric mucosa (16,22). Bacterial attachment causes induction of interleukin-8 (IL-8) release, and Cag proteins evoke activation of nuclear factor-B and release of IL-8 that may support leukocyte attachment during inflammation (8,17,21). However, the bacterial antigens associated with inflammation are still not clear.Heat shock proteins (hsp's) are immunodominant antigens in various diseases including H. pylori infection (4, 26). Our previous studies have shown that the 60-kDa hsp (hsp60) is expressed in the follicular dendritic cells of the gastric mucosa in patients with gastric MALT lymphoma (15) and that antibodies to human hsp60 can be detected in MALT lymphoma patients (14). These results indicate that hsp60 may be associated with the host immune reaction in H. pylori infection, specifically, the pathogensis of gastric MALT lymphoma.In the present study, H. pylori hsp60 recombinant protein was expressed, and the levels of immunoglobulin G (IgG), IgG1, IgG2, IgM, and IgA antibodies to hsp60 in sera were measured in patients with gastric ulcer, duodenal ulcer, gastritis, and gastric MALT lympho...

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Section: Vol 8 2001supporting

confidence: 91%

Immunoglobulin G1 Antibody Response toHelicobacter pyloriHeat Shock Protein 60 Is Closely Associated with Low-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

Ishii

Yokota

Sugiyama

et al. 2001

Clin Diagn Lab Immunol

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“…Among the subjects, the degree of passive translocation into NLF apparently fluctuated to a large extent (undetectable to 16% for IgE, 0.003% to 5.1% for IgG), so the absolute concentrations of IgE and IgG could not be quantitatively compared. Instead, the relative specific antibody proportions in total IgE and IgG deserved quantitative comparisons in a similar way as a previous study (7,19). The specific antibody amounts were estimated depending on the standard curves for total immunoglobulins of each class.…”

Section: Resultsmentioning

confidence: 98%

“…2). The results about JCP-specific IgA were in good agreement with a previous report about Helicobactor pyrolispecific IgA in gastric juice (7). However, the mean ratio of specific proportions in the NLF to in serum was lower in IgA, compared with IgE (Table 1).…”

Section: Discussionmentioning

confidence: 99%

A Quantitative Analysis of Cedar Pollen‐Specific Immunoglobulins in Nasal Lavage Supported the Local Production of Specific IgE, Not of Specific IgG

Yoshida

Usui

Kusumi

et al. 2005

Microbiology and Immunology

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Many studies have proved the relevance of local immune responses, rather than systemic immunity, to the pathogenesis of allergic rhinitis. Indeed, allergen‐specific B lymphocyte undergoes class switching to IgE in situ. However, the relative contribution of in situ production to the amount in serum is still ambiguous. Here, a quantitative comparison of the local concentration of allergen‐specific IgE with the systemic concentration was explored for the estimation. Among seasonal rhinitis patients, total and Japanese cedar pollen (JCP)‐specific IgE, IgA and IgG antibodies were quantified in nasal lavage fluid (NLF) and serum with the time‐resolved fluorescence immunosorbent assay. Although the total amounts of IgE and IgG classes in the NLF, which were apparently passive discharge from the mucosal tissue, were smaller and variable, the relative proportions of JCP‐specific antibodies could be quantitatively compared between NLF and serum or between subjects. The proportions of specific IgE in the NLF were remarkably higher than in serum (average 13.2‐fold) in most subjects, which strongly supported the predominant in situ production of the specific IgE and subsequent dilutions in the systemic circulations. Similar but smaller values were obtained for IgA (average 3.7‐fold). In contrast, the specific proportions of IgG in the NLF were surprisingly consistent with serum (average 1.0‐fold), suggesting that the specific IgG was mostly produced in the downstream lymphoid organs. The local productions of specific IgE would encourage the topical therapies and the usage of the NLF for the diagnosis of allergic rhinitis.

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“…Several investigations, however, have demonstrated a strong relationship between gastric mucosal inflammation or ulceration and gastric juice antibodies directed against H. pylori antigens (13,23,55), as well as to culture and PCR analysis of H. pylori obtained directly from gastric juice (1). These studies suggest that specific gastric juice antibody responses (mainly secretory immunoglobulin A [IgA]) to CagA may serve as biomarkers for pathological outcome.…”

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confidence: 99%

Local and Systemic Immune and Inflammatory Responses to Helicobacter pylori Strains

Bhat

Gaensbauer

Peek

et al. 2005

Clin Vaccine Immunol

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Colonization with Helicobacter pylori eventuates in varied clinical outcomes, which relate to both bacterial and host factors. Here we examine the relationships between cagA status, serum and gastric juice antibody responses, and gastric inflammation in dyspeptic patients. Serum, gastric juice, and gastric biopsy specimens were obtained from 89 patients undergoing endoscopy. H. pylori colonization and cagA status were determined by histology, culture, and PCR methods, and acute inflammation and chronic inflammation in the gastric mucosa were scored by a single pathologist. Serum and gastric juice antibodies to H. pylori whole-cell and CagA antigens were determined by enzyme-linked immunosorbent assay. Relationships between variables were sequentially analyzed using univariate and multivariate statistical methods. Of the 89 subjects, 62 were colonized by H. pylori. By univariate analyses, levels of serum immunoglobulin G (IgG) and IgA and gastric juice IgA antibodies against whole-cell and CagA antigens each were significantly higher in the H. pyloripositive group than in the H. pylori-negative group (P < 0.001). H. pylori and CagA seropositivities were both significantly associated with enhanced inflammation in gastric antrum and body (P < 0.02). The presence of gastric juice antibodies to H. pylori antigens was associated with more severe gastric inflammation. However, in multivariate analyses, only the presence of serum antibodies against CagA and, to a lesser extent, whole-cell antigens remained significantly associated with acute and chronic inflammation in antrum and body (P < 0.05). Thus, serum antibody response to CagA correlates with severity of gastric inflammation. Furthermore, given the relationships demonstrated by multivariate analysis, determination of gastric juice antibodies may provide a better representation of serum, rather than secretory, immune response.

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Quantitative detection of secretory immunoglobulin A to Helicobacter pylori in gastric juice: Antibody capture enzyme‐linked immunosorbent assay

Cited by 4 publications

References 0 publications

Immunoglobulin G1 Antibody Response toHelicobacter pyloriHeat Shock Protein 60 Is Closely Associated with Low-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

Immunoglobulin G1 Antibody Response toHelicobacter pyloriHeat Shock Protein 60 Is Closely Associated with Low-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

A Quantitative Analysis of Cedar Pollen‐Specific Immunoglobulins in Nasal Lavage Supported the Local Production of Specific IgE, Not of Specific IgG

Local and Systemic Immune and Inflammatory Responses to Helicobacter pylori Strains

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