Quantitative detection of platelet GPIIb-IIIa receptor antagonist activity using a flow cytometric method

Green, Lisa J.; Marder, Philip; Um, Suzane L.; Jakubowski, Joseph A.; Lawrence, Jeffry B.

doi:10.1002/(sici)1098-2825(1998)12:4<191::aid-jcla1>3.0.co;2-b

Cited by 6 publications

(1 citation statement)

References 29 publications

(31 reference statements)

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“…systems biology, scientific literature, or hypothesis generation), it is essential that biomarker leads be rapidly tested, placing a high demand on quantitative assays in various matrices. Assay types used for diagnostics and/or pharmaceutical development include competitive and sandwich ELISA [27], functional assays [28,29], flow cytometry [30], immunohistochemistry [31], and mass spectrometry [32]. While immunoassay-based methods generally involve measurement of a single marker, technologies that support concurrent measurement of numerous biomarkers are becoming more common [33].…”

Section: Clinical Endpointmentioning

confidence: 99%

The Role of Mass Spectrometry in Biomarker Discovery and Measurement

Ackermann¹,

Hale²,

Duffin³

2006

CDM

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Recent advances in the biological and analytical sciences have led to unprecedented interest in the discovery and quantitation of endogenous molecules that serve as indicators of drug safety, mechanism of action, efficacy, and disease state progression. By allowing for improved decision-making, these indicators, referred to as biomarkers, can dramatically improve the efficiency of drug discovery and development. Mass spectrometry has been a key part of biomarker discovery and evaluation owing to several important attributes, which include sensitive and selective detection, multi-analyte analysis, and the ability to provide structural information. Because of these capabilities, mass spectrometry has been widely deployed in search for new markers both through the analysis of large molecules (proteomics) and small molecules (metabonomics). In addition, mass spectrometry is increasingly being used to support quantitative measurement to assist in the evaluation and validation of biomarker leads. In this review, the dual role of mass spectrometry for biomarker discovery and measurement is explored for both large and small molecules by examining the key technologies and methods used along the continuum from drug discovery through clinical development.

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