Quantitative Detection of Extra Virgin Olive Oil Adulteration, as Opposed to Peanut and Soybean Oil, Employing LED-Induced Fluorescence Spectroscopy

Abstract

As it is high in value, extra virgin olive oil (EVOO) is frequently blended with inferior vegetable oils. This study presents an optical method for determining the adulteration level of EVOO with soybean oil as well as peanut oil using LED-induced fluorescence spectroscopy. Eight LEDs with central wavelengths from ultra-violet (UV) to blue are tested to induce the fluorescence spectra of EVOO, peanut oil, and soybean oil, and the UV LED of 372 nm is selected for further detection. Samples are prepared by mixing olive oil with different volume fractions of peanut or soybean oil, and their fluorescence spectra are collected. Different pre-processing and regression methods are utilized to build the prediction model, and good linearity is obtained between the predicted and actual adulteration concentration. This result, accompanied by the non-destruction and no pre-treatment characteristics, proves that it is feasible to use LED-induced fluorescence spectroscopy as a way to investigate the EVOO adulteration level, and paves the way for building a hand-hold device that can be applied to real market conditions in the future.

Fluorescence Spectroscopy Based Characterization of Flaxseed Oil

“…Fluorescence spectroscopy has been used extensively for the detection of adulteration of edible oils [27,28,[39][40][41][42] and applied here for the detection of adulteration in axseed oil. In order to detect the adulteration of canola oil in axseed oil, a methodology has been opted in which emission spectra of pure canola and axseed oil along with the adulterated samples have been measured and used with principal component analysis (PCA) for their detection [28,32].…”

Ultraviolet-induced fluorescence of oil spill recognition using a semi-supervised algorithm based on thickness and mixing proportion–emission matrices

“…However, due to their weak light intensity, the elimination of the fluorescence background is very demanding. 27 Despite this, a UV LED is used as the excitation light source in the laboratory experiments.…”

Applications of UV–Visible, Fluorescence and Mid-Infrared Spectroscopic Methods Combined with Chemometrics for the Authentication of Apple Vinegar

“…Spectroscopic techniques, used mostly as untargeted methods, have the advantages of being rapid and generating relatively less waste, and they produce fingerprints of the analyzed samples. They are also very suitable for use as sensors [ 11 , 12 , 13 ]. Since spectroscopic techniques produce a large number of variables, multivariate statistical analysis tools are commonly used to evaluate these data.…”