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IntroductionThe aim of this chapter is to provide an overview of the magnetic force microscopy ( MFM ) technique and its application for the characterization of magnetic nanoparticle s ( MNP s). The chapter also serves as a user ' s guide to MFM, without unnecessary repetition of previously published information, and without excessive mathematical detail. The reader is introduced to physical principals of MFM, the properties of MFM probes, probe calibration, methods of MFM detection, and application of MFM for MNPs. The goal is not to provide a detailed overview of MFM and hardware design, as several excellent texts (cited in the references) are available for this purpose. Rather, the special considerations and challenges required for MFM studies of MNPs, especially in biological samples, are highlighted. The chapter concludes with some details of the recent developments and future trends in MFM of MNPs for the life sciences.
Development of MFMUnderstanding the nature of magnetism at the nanometer -length scale is of interest from a fundamental perspective, as well as for the development of next generation of MNPs for the life sciences. The study of MNPs involves special challenges since, below a critical dimension, the competition between magnetostatic energy and exchange energy is predicted to suppress magnetic domain formation, leading to single -domain structures. These single -domain MNPs not only possess low magnetization values, as compared to bulk material, but may also have lower coercivity or a superparamagnetic character at temperatures conducive to living systems. Thus, specialized techniques are needed to understand and characterize the magnetic nature of MNPs.