Quantitative CT imaging of the spatio-temporal distribution patterns of vasa vasorum in aortas of apoE−/−/LDL−/− double knockout mice

Kampschulte, Marian; Brinkmann, A; Stieger, Philipp; Sedding, Daniel; Dierkes, Christian; Bohle, Rainer M.; Krombach, Gabriele A.; Ritman, Erik L.; Langheinrich, Alexander C.

doi:10.1016/j.atherosclerosis.2010.07.010

Cited by 16 publications

(16 citation statements)

References 20 publications

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“…There are several different 3D imaging techniques that can be used to examine the mouse brain at the mesoscopic scale. Computed tomography is one example, which is often used to examine high-density structures like bone or vascular trees (filled with X-ray opaque contrast agents) [94][95][96], and, more recently, examine mouse embryos, which relies on the use of an iodine contrast agent to enhance the soft tissue contrast [97][98][99]. Ultrasound biomicroscopy, commonly used for cardiac imaging in the mouse [100][101][102], is also useful for studying embryonic development [103].…”

Section: Discoveries Of Neuroanatomical Abnormalities In Mouse Modelsmentioning

confidence: 99%

Behavioral and Neuroanatomical Phenotypes in Mouse Models of Autism

2015

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In order to understand the consequences of the mutation on behavioral and biological phenotypes relevant to autism, mutations in many of the risk genes for autism spectrum disorder have been experimentally generated in mice. Here, we summarize behavioral outcomes and neuroanatomical abnormalities, with a focus on highresolution magnetic resonance imaging of postmortem mouse brains. Results are described from multiple mouse models of autism spectrum disorder and comorbid syndromes, including the 15q11-13, 16p11.2, 22q11.2, Cntnap2, Engrailed2, Fragile X, Integrinβ3, MET, Neurexin1a, Neuroligin3, Reelin, Rett, Shank3, Slc6a4, tuberous sclerosis, and Williams syndrome models, and inbred strains with strong autism-relevant behavioral phenotypes, including BTBR and BALB. Concomitant behavioral and neuroanatomical abnormalities can strengthen the interpretation of results from a mouse model, and may elevate the usefulness of the model system for therapeutic discovery.

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Section: Discoveries Of Neuroanatomical Abnormalities In Mouse Modelsmentioning

confidence: 99%

Behavioral and Neuroanatomical Phenotypes in Mouse Models of Autism

2015

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“…In summary, micro-CT has demonstrated superior spatial resolution which allows for quantitative imaging of the spatial and temporal distribution of vasa vasorum in the aorta and coronary artery [15, 17, 23, 27]. Vasa vasorum parameters including vasa vasorum count, vasa vasorum spatial density, vasa vasorum vascular area fraction, and vasa vasorum endothelial surface fraction can be reliably evaluated with use of micro-CT [18, 26, 27].…”

Section: Imaging Modalities In the Visualization Of Vasa Vasorum Amentioning

confidence: 99%

“…Vasa vasorum parameters including vasa vasorum count, vasa vasorum spatial density, vasa vasorum vascular area fraction, and vasa vasorum endothelial surface fraction can be reliably evaluated with use of micro-CT [18, 26, 27]. The increase in arterial wall opacity due to increased vascularity of the vasa vasorum can also be detected on micro-CT images, and this serves as an index of the angiogenesis that takes places in early plaque development [28].…”

Section: Imaging Modalities In the Visualization Of Vasa Vasorum Amentioning

confidence: 99%

Atherosclerosis and Atheroma Plaque Rupture: Imaging Modalities in the Visualization of Vasa Vasorum and Atherosclerotic Plaques

Sun

2014

The Scientific World Journal

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Invasive angiography has been widely accepted as the gold standard to diagnose cardiovascular pathologies. Despite its superior resolution of demonstrating atherosclerotic plaque in terms of degree of lumen stenosis, the morphological assessment for the plaque is insufficient for the analysis of plaque components, and therefore, unable to predict the risk status or vulnerability of atherosclerotic plaque. There is an increased body of evidence to show that the vasa vasorum play an important role in the initiation, progression, and complications of atherosclerotic plaque leading to major adverse cardiac events. This paper provides an overview of the evidence-based reviews of various imaging modalities with regard to their potential value for comprehensive characterization of the composition, burden, and neovascularization of atherosclerotic plaque.

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“…In previous studies regarding plaque differentiation of atherosclerotic vascular wall lesions, quantitative statements regarding plaque composition differentiating fibrous, calcified, and lipid-rich plaques could be made in micro-CT studies [16]. The distribution, size, and type of atherosclerotic plaque can also be detected and histologically verified via nano-CT equivalent to micro-CT. Calcifying, fibrotic, and hemorrhagic plaques can be reliably differentiated on the basis of the vasa vasorum density, the X-ray opacity, and the object size [17]. Based on the improved spatial resolution in nano-CT, post-hemorrhagic iron accumulations can be identified on the basis of their fine-granular morphology ( • " Fig.…”

Section: Sample Mountingmentioning

confidence: 99%

“…The improved detection of details demonstrated for nano-CT makes it possible to address issues that could not be or could only be insufficiently examined under the previous device-specific resolution limits in computed tomography. The visualization of soft cellular lacunae in calcified tissues [17,28] provides information that characterizes the interaction between cells and an extracellular matrix in the three-dimensional space. In addition, it shows the necessity for the development and researching of new contrast agents that allow differentiation of cells and the extracellular matrix in soft tissue, i. e., in the low contrast range [29].…”

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confidence: 99%