Quantitative Content Parameter in the Standardization of Veratrum Aqua, Veratrum Lobelianum Bernh. Based Drug

Melnik, E. V.; Белова, М. В.; Тюрин, Ю А; Раменская, Г. В.

doi:10.33380/2305-2066-2021-10-1-107-113

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…Veratrum lobelianum is a robust herbaceous plant found in Northeast Asia, Central Europe, and North America that is known for its toxicity in humans and animals [33]. The alkaloid jervine, abundant in V. lobelianum, exhibits antitumor effects and influences DNA damage repair in nasopharyngeal carcinoma cells [24,27,34]. Our experiments, using jervine-containing V. lobelianum extract, activated the DNA damage checkpoint by downregulating hedgehog signaling, suggesting a complex relationship (Figures 3 and 4).…”

Section: Lobelianummentioning

confidence: 72%

From Hemp Waste to Bioactive Nanofiber Composites: Deep Eutectic Solvents and Electrospinning in Upcycling Endeavors

Mouro,

Gomes,

Gouveia

2023

Preprint

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Natural fibers have attracted increasing interest as an alternative to produce environmentally friendly and sustainable materials. Particularly, hemp fibers have revealed to be widely used in various industrial applications due to their extremely unique properties. However, hemp can generate a large amount of agro-waste, and results in an attractive source of biopolymers for the development of low-cost materials in alternative to the raw materials and conventional petroleum-based plastics. In addition, deep eutectic solvents (DESs), a new type of truly green solvents, have been tested to remove gums, lignin, and other non-cellulosic components from hemp fibers. Reusing these components dissolved into the DESs to fabricate new materials directly by electrospinning is a very attractive but still unexplored endeavor. Thus, this innovative research to venture new upcycling pathways is focused on the fabrication of composite nanofibers by electrospinning of a gel-based blend of Poly(vinyl alcohol) (PVA) and hemp agro-waste (HW) dissolved into choline chloride (ChCl) : Glycerol (1:2) and ChCl : Urea (1:2) DES mixtures. The results obtained revealed that the produced nanofibers displayed uniform appearance with diameters ranging from 257.72 ± 65.63 nm to 380.78 ± 133.98 nm. In addition, the mechanical properties of the electro-spun composite nanofibers produced from the gel-based blends of HW dissolved in DESs and PVA (HW-DESs_PVA) were found to be superior, resulting in an enhanced tensile strength and Young’s modulus. Furthermore, the incorporation of HW into the nanofibers was able to provide bioactive antioxidant and antibacterial properties. Notably, Onobrychis cornuta and Veratrum lobelianum extracts activated a DNA damage checkpoint response via the ATM/ATR and CHK-1 pathways; hindering germline development. Our LC‒MS analysis revealed jervine in V. lobelianum and nine antitumor compounds in O. cornuta. Interestingly, linoleic acid replicated phenotypes induced by O. cornuta exposure, including an increased level of pCHK-1 foci, apoptosis, and the MAPK pathway. We observed that mutants in the MAPK pathway suppressed the reduction in worm survival, emphasizing its role in survivability, meiotic progression, and DNA damage checkpoint-mediated apoptosis. This study reveals complex interactions between herbal extracts and C. elegans processes, shedding light on potential antitumor effects and mechanisms. The findings provide insights into the complex landscape of herbal medicine's impact on a model organism, offering implications for broader applications.

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Section: Lobelianummentioning

confidence: 72%

From Hemp Waste to Bioactive Nanofiber Composites: Deep Eutectic Solvents and Electrospinning in Upcycling Endeavors

Mouro,

Gomes,

Gouveia

2023

Preprint

View full text Add to dashboard Cite

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“…Veratrum lobelianum is a robust herbaceous plant found in Northeast Asia, Central Europe, and North America that is known for its toxicity in humans and animals [ 33 ]. The alkaloid jervine, abundant in V. lobelianum , exhibits antitumor effects and influences DNA damage repair in nasopharyngeal carcinoma cells [ 24 , 27 , 34 ]. Our experiments, using jervine-containing V. lobelianum extract, activated the DNA damage checkpoint by downregulating hedgehog signaling, suggesting a complex relationship ( Figure 3 and Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Exploring the Impact of Onobrychis cornuta and Veratrum lobelianum Extracts on C. elegans: Implications for MAPK Modulation, Germline Development, and Antitumor Properties

Meng,

Pathak,

Ren

et al. 2023

Nutrients

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In an era of increasing interest in the potential health benefits of medicinal foods, the need to assess their safety and potential toxicity remains a critical concern. While these natural remedies have garnered substantial attention for their therapeutic potential, a comprehensive understanding of their effects on living organisms is essential. We examined 316 herbal extracts to determine their potential nematocidal attributes in Caenorhabditis elegans. Approximately 16% of these extracts exhibited the capacity to induce diminished survival rates and larval arrest, establishing a correlation between larval arrest and overall worm viability. Certain extracts led to an unexpected increase in male nematodes, accompanied by a discernible reduction in DAPI-stained bivalent structures and perturbed meiotic advancement, thereby disrupting the conventional developmental processes. Notably, Onobrychis cornuta and Veratrum lobelianum extracts activated a DNA damage checkpoint response via the ATM/ATR and CHK-1 pathways, thus hindering germline development. Our LC–MS analysis revealed jervine in V. lobelianum and nine antitumor compounds in O. cornuta. Interestingly, linoleic acid replicated phenotypes induced by O. cornuta exposure, including an increased level of pCHK-1 foci, apoptosis, and the MAPK pathway. Mutants in the MAPK pathway mitigated the decline in worm survival, underscoring its importance in promoting worm viability. This study reveals complex interactions between herbal extracts and C. elegans processes, shedding light on potential antitumor effects and mechanisms. The findings provide insights into the complex landscape of herbal medicine’s impact on a model organism, offering implications for broader applications.

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“…The major alkaloid components of Veratrum aqua are jervine, protoveratrine A (proA), and protoveratrine B (proB), that are classified as Veratrum alkaloids [ 15 ]. Nevertheless, the presence of these alkaloids is not taken into account in Veratrum aqua specification.…”

Section: Introductionmentioning

confidence: 99%

Insights into the Cardiotoxic Effects of Veratrum Lobelianum Alkaloids: Pilot Study

Taldaev

Terekhov

Melnik

et al. 2022

Toxins

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Jervine, protoveratrine A (proA), and protoveratrine B (proB) are Veratrum alkaloids that are presented in some remedies obtained from Veratrum lobelianum, such as Veratrum aqua. This paper reports on a single-center pilot cardiotoxic mechanism study of jervine, proA, and proB in case series. The molecular aspects were studied via molecular dynamic simulation, molecular docking with cardiac sodium channel NaV1.5, and machine learning-based structure–activity relationship modeling. HPLC-MS/MS method in combination with clinical events were used to analyze Veratrum alkaloid cardiotoxicity in patients. Jervine demonstrates the highest docking score (−10.8 kcal/mol), logP value (4.188), and pKa value (9.64) compared with proA and proB. Also, this compound is characterized by the lowest calculated IC50. In general, all three analyzed alkaloids show the affinity to NaV1.5 that highly likely results in cardiotoxic action. The clinical data of seven cases of intoxication by Veratrum aqua confirms the results of molecular modeling. Patients exhibited nausea, muscle weakness, bradycardia, and arterial hypotension. The association between alkaloid concentrations in blood and urine and severity of patient condition is described. These experiments, while primary, confirmed that jervine, proA, and proB contribute to cardiotoxicity by NaV1.5 inhibition.

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Quantitative Content Parameter in the Standardization of Veratrum Aqua, Veratrum Lobelianum Bernh. Based Drug

Cited by 3 publications

References 5 publications

From Hemp Waste to Bioactive Nanofiber Composites: Deep Eutectic Solvents and Electrospinning in Upcycling Endeavors

From Hemp Waste to Bioactive Nanofiber Composites: Deep Eutectic Solvents and Electrospinning in Upcycling Endeavors

Exploring the Impact of Onobrychis cornuta and Veratrum lobelianum Extracts on C. elegans: Implications for MAPK Modulation, Germline Development, and Antitumor Properties

Insights into the Cardiotoxic Effects of Veratrum Lobelianum Alkaloids: Pilot Study

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