Quantitative confocal phase imaging by synthetic optical holography

Schnell, Martin; Perez-Roldan, M. J.; Carney, P. Scott; Hillenbrand, Rainer

doi:10.1364/oe.22.015267

Cited by 32 publications

(37 citation statements)

References 22 publications

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“…It was demonstrated that SOH is a method technically simple and fast for phase imaging. 9,10 It can be integrated in scanning microscopes by simply adding a linearly moving reference mirror, which generates a synthetic reference wave, analogous to the plane reference wave of wide-field off-axis holography. The present work describes a synthetic holography system, implemented through a lens-free microscope based on a low-coherence scanning optical micro-cavity.…”

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confidence: 99%

“…The direct term U S was filtered, demodulated and subsequently an inverse FT was performed. In image recovering, we exploit only the term of first order appearing in Equation (9), neglecting the higher orders that are strongly attenuated. The above results confirm the potentialities of this technique in realizing imaging at multi-wavelengths with just one scan.…”

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confidence: 99%

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Synthetic holography based on scanning microcavity

Donato

Farina

2015

AIP Advances

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Synthetic optical holography (SOH) is an imaging technique, introduced in scanning microscopy to record amplitude and phase of a scattered field from a sample. In this paper, it is described a novel implementation of SOH through a lens-free low-coherence system, based on a scanning optical microcavity. This technique combines the low-coherence properties of the source with the mutual interference of scattered waves and the resonant behavior of a micro-cavity, in order to realize a high sensitive imaging system. Micro-cavity is compact and realized by approaching a cleaved optical fiber to the sample. The scanning system works in an open-loop configuration without the need for a reference wave, usually required in interferometric systems. Measurements were performed over calibration samples and a lateral resolution of about 1 μm is achieved by means of an optical fiber with a Numerical Aperture (NA) equal to 0.1 and a Mode Field Diameter (MDF) of 5.6 μm.

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confidence: 99%

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confidence: 99%

Synthetic holography based on scanning microcavity

Donato

Farina

2015

AIP Advances

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“…The second way is introduced by moving the reference arm. Although this method has been applied to SOH and Fourier-Domain optical coherence tomography [11,19], application of it into the LCHM yields a high-speed en-face complex confocal imaging technique. Using a custom axial height phantom fabricated using chrome deposition, we have demonstrated that both off-axis and in-line LCHMs yielded variations in phase corresponding to heights in the ~100 nm range with a contrast-to-noise ratio of ~31 dB.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore it is limited to static samples. In 2014, synthetic optical holography (SOH) was proposed to achieve quantitative confocal phase imaging by moving a reference mirror to introduce a spatial frequency carrier [11]. SOH realized one single-shot hologram for one en-face confocal image thereby reducing the data flow by 6 orders of magnitude compared to DCM.…”

Section: Introductionmentioning

confidence: 99%

“…Ultrahigh temporal resolution M-mode is demonstrated by measuring the vibration of a PZT-actuated mirror driven by a sine wave at 1000Hz. Coherent detection in the LCHM can also be realized in a in-line optical configuration by moving the reference beam as demonstrated in SOH and spectral-domain optical coherence tomography [11,19]. Doppler frequency shift due to the moving reference beam can introduce the spatial fringes along the scanning direction.…”

Section: Introductionmentioning

confidence: 99%

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High-speed line-field confocal holographic microscope for quantitative phase imaging

et al. 2016

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Abstract:We present a high-speed and phase-sensitive reflectance linescanning confocal holographic microscope (LCHM). We achieved rapid confocal imaging using a fast line-scan CCD camera and quantitative phase imaging using off-axis digital holography (DH) on a 1D, line-by-line basis in our prototype experiment. Using a 20 kHz line scan rate, we achieved a frame rate of 20 Hz for 512x512 pixels en-face confocal images. We realized coherent holographic detection two different ways. We first present a LCHM using off-axis configuration. By using a microscope objective of a NA 0.65, we achieved axial and lateral resolution of ~3.5 micrometers and ~0.8 micrometers, respectively. We demonstrated surface profile measurement of a phase target at nanometer precision and the digital refocusing of a defocused confocal en-face image. Ultrahigh temporal resolution M mode is demonstrated by measuring the vibration of a PZTactuated mirror driven by a sine wave at 1 kHz. We then report our experimental work on a LCHM using an in-line configuration. In this inline LCHM, the coherent detection is enabled by moving the reference arm at a constant speed, thereby introducing a Doppler frequency shift that leads to spatial interference fringes along the scanning direction. Lastly, we present a unified formulation that treats off-axis and in-line LCHM in a unified joint spatiotemporal modulation framework and provide a connection between LCHM and the traditional off-axis DH. The presented high-speed LCHM may find applications in optical metrology and biomedical imaging.

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Two-pack frequency-selective incoherent holography by using a dual-beam setup

Zhang

Shi

et al. 2022

Optics and Lasers in Engineering

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Quantitative confocal phase imaging by synthetic optical holography

Cited by 32 publications

References 22 publications

Synthetic holography based on scanning microcavity

Synthetic holography based on scanning microcavity

High-speed line-field confocal holographic microscope for quantitative phase imaging

Two-pack frequency-selective incoherent holography by using a dual-beam setup

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