Quantitative competitive PCR for the detection of genetically modified organisms in food

Hübner, Philipp; Studer, Edgar; Lüthy, Jürg

doi:10.1016/s0956-7135(99)00074-2

Cited by 55 publications

(21 citation statements)

References 14 publications

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“…PCR has been repeatedly used to analyze food samples qualitatively for recombinant DNA sequences [3,4]. Quantification of contaminations with genetically modified organisms was done by competitive PCR [5,6], PCR-ELISA [7], or real-time PCR (e. g. [8]). In these cases, DNA preparation has been performed by conventional means.…”

Section: Introductionmentioning

confidence: 99%

Automated DNA preparation from maize tissues and food samples suitable for real-time PCR detection of native genes

Hahnen¹,

Offermann²,

Miedl

et al. 2002

European Food Research and Technology

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There is an increasing need for high-throughput analyses of plants and food samples for the presence of specific DNA sequences, e.g. transgenic contaminations. We developed and optimized conditions for the automated isolation of DNA from several maize tissues and various edibles containing maize using the MagNA Pure LC system (Roche Applied Science). Our results show that the system provided is capable of isolating DNA from any tested source. Quantification of an endogenous gene by LightCycler real-time PCR revealed that the DNA is suitable in quality and quantity for multiple PCR analyses.

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Section: Introductionmentioning

confidence: 99%

Automated DNA preparation from maize tissues and food samples suitable for real-time PCR detection of native genes

Hahnen¹,

Offermann²,

Miedl

et al. 2002

European Food Research and Technology

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“…The most common quantitative PCR approach is real-time PCR; however, other approaches such as quantitative competitive PCR have also been used for relative quantification of GM events (Hübner et al 1999). In real-time PCR, the accumulation of amplification products is monitored over time during the PCR.…”

Section: Dna-based Analysismentioning

confidence: 99%

Adventitious presence of GMOs: Scientific overview for Canadian grains

Demeke¹,

Perry²,

Scowcroft³

2006

Can. J. Plant Sci.

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. 2006. Adventitious presence of GMOs: Scientific overview for Canadian grains. Can. J. Plant Sci. 86: 1-23. The global expansion in the development and cultivation of genetically modified (GM) crops has increased international concern about adventitious presence of GM materials in non-GM seeds and grains. GM events in canola, corn, soybean, cotton, flax, papaya, potato, squash, sugar beet, and tomato have received regulatory approval in Canada. However, GM cultivars are only in commercial production for canola, corn and soybean. More than 30 GM events have been approved in these three crops. Cases of unapproved adventitious presence of GM materials that have impacted grain trading and handling in Canada and other countries include StarLink™ corn, GT200 canola, GM canola in mustard and recently Bt10 corn. Some countries have established tolerance and traceability requirements for adventitious presence of GMOs, while others are in the process of developing or adopting legislation. The threshold for labeling of adventitious presence of approved GM material in non-GM grain varies from 0.9% (e.g., EU) to 5% (e.g., Japan). Progress has been made in the development of DNA-and proteinbased GMO detection methods. However, only a limited number of these detection methods have been internationally validated. The challenges for detection methods include sampling, a lack of certified reference material, a lack of DNA sequence information for the design of event-specific primers, and the sheer number of individual events that may be present and tested for. Current efforts by ISO and CEN will be valuable for establishing harmonized and standardized GMO detection methods.Key words: List of GM events, cases of AP, tolerance, traceability, detection methods, challenges Demeke, T., Perry, D. J. et Scowcroft, W. R. 2006. Présence de matériel génétiquement modifié adventif : survol de la situation des grains au Canada. Can. J. Plant Sci. 86: 1-23. Le développement et la culture de matériel génétiquement modifié (GM) connaissent une expansion mondiale si bien que, partout sur terre, on s'inquiète de la présence de matériel GM adventif dans les semences et les grains non GM. La modification génétique du canola, du maïs, du soja, du coton, du lin, de la papaye, de la pomme de terre, de la courge, de la betterave sucrière et de la tomate a été autorisée par les organismes de réglementation au Canada. Les cultivars GM cultivés commercialement se limitent néanmoins au canola, au maïs et au soja. Plus de 30 modifications génétiques ont été approuvées pour ces trois cultures. Parmi les cas de matériel GM adventif non autorisé qui ont posé des problèmes au niveau des échanges commerciaux et de la manutention du grain au Canada et ailleurs, on retrouve le maïs StarLink MC , le canola GT200, le canola GM dans la moutarde et, plus récemment, le maïs Bt10. Certains pays tolèrent ou exigent qu'on puisse retracer le matériel GM adventif, mais d'autres ont entrepris l'élaboration ou l'adoption de lois en la matière. Le seuil de tolérance concernan...

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“…Ratios of signal intensities of competitor and target amplicons measured after gel electrophoresis are plotted logarithmically against amounts of competitor initially used in the sample. The point of equivalence is determined by the intersection of the linear regression curve and the abscissa [95].…”

Section: Competitive Pcrmentioning

confidence: 99%

Methods for Detection of Genetically Modified Organisms in Composite and Processed Foods

Engel

Moreano²,

Ehlert

2006

Genetically Engineered Food

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Quantitative competitive PCR for the detection of genetically modified organisms in food

Cited by 55 publications

References 14 publications

Automated DNA preparation from maize tissues and food samples suitable for real-time PCR detection of native genes

Automated DNA preparation from maize tissues and food samples suitable for real-time PCR detection of native genes

Adventitious presence of GMOs: Scientific overview for Canadian grains

Methods for Detection of Genetically Modified Organisms in Composite and Processed Foods

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