Quantitative comparison of stem cell marker-positive cells in fetal and term human amnion

M, Izumi; Pazin, Benjamin J.; Minervini, Crescenzio Francesco; Gerlach, Jörg C.; Ross, Mark A.; Stolz, Donna B.; Turner, Morris E.; Thompson, Robert L.; Miki, Toshio

doi:10.1016/j.jri.2009.02.007

Cited by 55 publications

(45 citation statements)

References 18 publications

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“…However, few studies to date have clarified whether the gestational stage of isolation may affect the differentiation potential of AECs [31, 44]. The results obtained in the present study show that sheep AECs greatly changed their phenotype, stemness properties, as well as, their in vitro plasticity during gestation, while they maintained unaltered the immunomodulatory activities.…”

Section: Discussionmentioning

confidence: 50%

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Gestational stage affects amniotic epithelial cells phenotype, methylation status, immunomodulatory and stemness properties

Barboni

Russo

Curini

et al. 2014

Stem Cell Rev and Rep

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Stem cells isolated from amniotic epithelium (AECs) have shown great potential in cell-based regenerative therapies. Because of their fetal origin, these cells exhibit elevated proliferation rates and plasticity, as well as, immune tolerance and anti-inflammatory properties. These inherent attitudes make AECs well-suited for both allogenic and xenogenic cellular transplants in animal models. Since in human only at term amnion is easily obtainable after childbirth, limited information are so far available concerning the phenotypic and functional difference between AECs isolated from early and late amnia. To this regard, the sheep animal model offers an undoubted advantage in allowing the easy collection of both types of AECs in large quantity. The aim of this study was to determine the effect of gestational age on ovine AECs (oAECs) phenotype, immunomodulatory properties, global DNA methylation status and pluripotent differentiation ability towards mesodermic and ectodermic lineages. The immunomodulatory property of oAECs in inhibiting lymphocyte proliferation was mainly unaffected by gestational age. Conversely, gestation considerably affected the expression of surface markers, as well the expression and localization of pluripotency markers. In detail, with progression of gestation the mRNA expression of NANOG and SOX2 markers was reduced, while the ones of TERT and OCT4A was unaltered; but at the end of gestation NANOG, SOX2 and TERT proteins mainly localized outside the nuclear compartment. Regarding the differentiation ability, LPL (adipogenic-specific gene) mRNA content significantly increased in oAECs isolated from early amnia, while OCN (osteogenic-specific gene) and NEFM (neurogenic-specific gene) mRNA content significantly increased in oAECs isolated from late amnia, suggesting that gestational stage affected cell plasticity. Finally, the degree of global DNA methylation increased with gestational age. All these results indicate that gestational age is a key factor capable of influencing morphological and functional properties of oAECs, and thus probably affecting the outcome of cell transplantation therapies.

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Section: Discussionmentioning

confidence: 50%

“…In fact, Nakajima et al [30] demonstrated a reduction of telomerase activity in rat AECs collected at the end of gestation, while Izumi et al [31] showed that there is a reduced expression in stem cell markers in human term amnion compared to fetal ones.…”

Section: Introductionmentioning

confidence: 99%

Gestational stage affects amniotic epithelial cells phenotype, methylation status, immunomodulatory and stemness properties

Barboni

Russo

Curini

et al. 2014

Stem Cell Rev and Rep

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“…To determine whether HCMV infects AmEpCs, primary cells isolated from mid-gestation and late gestation placentas were infected with VR1814. Immunostaining of control AmEpCs, which have the properties of fetal stem cells, 22,29,44 revealed expression of the pluripotency proteins Oct4 and SOX2, the embryonic stem celle specific surface antigen stage-specific embryonic antigen-4, 45 and the transcription factors GATA-3 and Hand-1, regulators of cell differentiation 44 ( Figure 2A). Late in infection, when gB and pp28 were expressed, Oct4, SOX2, stage-specific embryonic antigen-4, GATA3, and Hand1 were down-regulated (Figure 2A).…”

Section: Persistent Hcmv Infection In Primary Amepcs In Vitromentioning

confidence: 99%

Persistent Cytomegalovirus Infection in Amniotic Membranes of the Human Placenta

Tabata

Petitt

Fang-Hoover

et al. 2016

The American Journal of Pathology

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Human cytomegalovirus (HCMV) is the leading viral cause of birth defects, including microcephaly, neurological deficits, hearing impairment, and vision loss. We previously reported that epithelial cells in amniotic membranes of placentas from newborns with intrauterine growth restriction and underlying congenital HCMV infection contain viral proteins in cytoplasmic vesicles. Herein, we immunostained amniotic membranes from 51 placentas from symptomatic and asymptomatic congenital infection with HCMV DNA in amniotic fluid and/or newborn saliva, intrauterine growth restriction, preterm deliveries, and controls. We consistently observed HCMV proteins in amniotic epithelial cells (AmEpCs) from infected placentas, sometimes with aberrant morphology. Primary AmEpCs isolated from mid-gestation placentas infected with pathogenic VR1814 proliferated and released infectious progeny for weeks, producing higher virus titers than late-gestation cells that varied by donor. In contrast to intact virion assembly compartments in differentiated retinal pigment epithelial cells, infected AmEpCs made dispersed multivesicular bodies. Primary AmEpCs and explants of amniochorionic membranes from midgestation placentas formed foci of infection, and interferon-b production was prolonged. Infected AmEpCs up-regulated anti-apoptotic proteins survivin and Bcl-x L by mechanisms dependent and independent of the activated STAT3. Amniotic membranes naturally expressed both survivin and Bcl-x L , indicating that fetal membranes could foster persistent viral infection. Our results suggest strengthening innate immune responses and reducing viral functions could suppress HCMV infection in the fetal compartment. (Am J Pathol 2016 http://dx

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“…More recently, comparative analysis of hAEC from amnion of early-stage pregnancies and from term amnion showed that expression of the stem cell-specifi c cell surface markers TRA1-60 and TRA1-81, and of the molecular markers of pluripotency Nanog and Sox2, are all signifi cantly higher in fetal amnion, while expression of Oct-4 mRNA is similar between cells obtained either from fetal or term amnion [ 20 ].…”

Section: Mechanisms Of Infl Ammatory Disease: Will An Increased Undermentioning

confidence: 99%

Toward Cell Therapy Using Placenta-Derived Cells: Disease Mechanisms, Cell Biology, Preclinical Studies, and Regulatory Aspects at the Round Table

Parolini

Alviano

Bergwerf

et al. 2010

Stem Cells and Development

124

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Among the many cell types which may prove useful to regenerative medicine, mounting evidence suggests that human term placenta-derived cells will join the list of significant contributors. In making new cell therapy-based strategies a clinical reality, it is fundamental that no a priori claims are made regarding which cell source is preferable for a particular therapeutic application. Rather, ongoing comparisons of the potentiality and characteristics of cells from different sources should be made to promote constant improvement in cell therapies, and such comparisons will likely show that individually-tailored cells can address disease-specific clinical needs. The principle underlying such an approach is resistance to the notion that comprehensive characterization of any cell type has been achieved, neither in terms of phenotype nor risks-to-benefits ratio. Tailoring cell therapy approaches to specific conditions also requires an understanding of basic disease mechanisms and close collaboration between translational researchers and clinicians, to identify current needs and shortcomings in existing treatments. To this end, the international workshop entitled "Placenta-derived stem cells for treatment of inflammatory diseases: moving toward clinical application" was held in Brescia, Italy, in March 2009, and aimed to harness an understanding of basic inflammatory mechanisms inherent in human diseases with updated findings regarding biological and therapeutic properties of human placenta-derived cells, with particular emphasis on their potential for treating inflammatory diseases. Finally, steps required to allow their future clinical application according to regulatory aspects including good manufacturing practice (GMP) were also considered. In September, 2009, the International Placenta Stem Cell Society (IPLASS) was founded to help strengthen the research network in this field.

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Quantitative comparison of stem cell marker-positive cells in fetal and term human amnion

Cited by 55 publications

References 18 publications

Gestational stage affects amniotic epithelial cells phenotype, methylation status, immunomodulatory and stemness properties

Gestational stage affects amniotic epithelial cells phenotype, methylation status, immunomodulatory and stemness properties

Persistent Cytomegalovirus Infection in Amniotic Membranes of the Human Placenta

Toward Cell Therapy Using Placenta-Derived Cells: Disease Mechanisms, Cell Biology, Preclinical Studies, and Regulatory Aspects at the Round Table

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