Quantitative Comparative Proteomic Analysis of Chicken Egg Vitelline Membrane Proteins during High-Temperature Storage

Abstract

To explore the thermally induced alterations in chicken egg vitelline membrane (CEVM) protein abundances, a comparative proteomic analysis of CEVM after 10 days of storage at 30 °C was performed. Altogether, 981 proteins were identified, of which 124 protein abundances were decreased and 79 were increased. Bioinformatic analysis suggested that the altered proteins were related to structure (n = 10), mechanical properties (n = 13), chaperone (n = 15), antibacterial (n = 12), and antioxidant (n = 3). Alterations in abundances of structural proteins, possibly resulting from the disintegration of these complexes, were observed in this study, suggesting a loss in fibrous structure. Several proteins involved in mechanical strength (n = 10), elasticity (n = 3), and chaperone were decreased in abundances, which indicated that deficits in these proteins might affect the CEVM mechanical properties. These findings will extend our understanding of CEVM deterioration during high-temperature storage from a proteomic perspective.

Comparative N-Glycoproteomic Analysis Provides Novel Insights into the Deterioration Mechanisms in Chicken Egg Vitelline Membrane during High-Temperature Storage

“…The CEVM samples were prepared according to the procedure used in our recent study. 1 Briefly, egg white and yolk were separated; then the yolk was washed with deionized water and the chalazae was cut off. The yolk was cut open to get the CEVM, and then CEVM was washed in a petri dish filled with deionized water at room temperature.…”

“…The CEVM protein extraction was performed according to previous methods. 1 Briefly, the CEVM samples were carefully ground into powder by liquid nitrogen. Then, 4 volumes of lysis buffer (8 M urea, 1% Protease Inhibitor Cocktail (Calbiochem)) was added to the CEVM samples, and then sonication for three times on ice was carried out using a high-intensity ultrasonic processor (Scientz, Ningbo, China) for these samples.…”

“…The maximum ion injection time was set to 200 ms and the fixed first mass was 100 m/z. 1 Database Search and Bioinformatic Analysis. The MS/MS data were processed using the Maxquant search engine (v.1.5.2.8 http://www.maxquant.org/) and then searched for matches with the UniProt (Gallus gallus, 27 804 sequences).…”

“…Recently, we have found that the thermally induced downregulation in abundance of CEVM strength and elasticity proteins may lead to the CEVM weakening. 1 Besides, a previous study demonstrated that the CEVM lost its selective nature and a spontaneous penetration of cholesterol into the albumen from yolk and CEVM was observed after 21 days during storage at 22 °C, increasing the permeability of the membrane for macromolecules. 5 However, the functionality of albumen is dependent on the quality of albumen proteins, and slight contamination with yolk can reduce the functionality of albumen, such as foaming properties.…”

Transcriptome and Proteome Analysis Revealed Key Pathways Regulating Final Stage of Oocyte Maturation of the Turkey (Meleagris gallopavo)

“…Candidate microarray analysis of the germinal disc region of chicken oocytes has revealed genes potentially involved in "laying" and "meat" line hen fertility [25], as well as a few genes involved in oocyte maturation and early embryo development in chicken hens [13]. Transcriptomic and proteomic analyses have been used to determine the mechanisms involved in chicken follicle selection [26][27][28], and proteomic analysis has been widely used for the analysis of the laid chicken egg vitelline membrane [29,30], and egg yolk [31] and egg white proteome [32]. However, there is a lack of similarly comprehensive studies for the turkey hen reproductive tract.…”