Quantitative changes of Holmes positive nucleolus-like inclusion bodies in the mouse locus coeruleus under various experimental conditions.

Katoh, Yoshimitsu; Shimizu, Nobuo

doi:10.1679/aohc.46.491

Cited by 4 publications

(2 citation statements)

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“…Our previous studies used experimental conditions such as dehydration/fasting or reserpine administration for mice, and the numbers of inclusion bodies decreased [29]. It was reported that the protein bodies were found to be severely de su nine), their place structure were different.…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that the protein bodies were found to be severely de su nine), their place structure were different. In addition, oth the inclusion bodies and pr bstantia nigra (SN) and LC in Parkinsonian brains [15] and disrupted in the LC in depression [30]. These findings point to the importance of the metabolic economy of catecholamine neurons since protein bodies in the SN and LC were lost in parallel with the dopamine loss in the SN, with the reduced concentrations of norepinephrine in the LC reported in Parkinsonian brains [31,32] and with disturbed metabolism of norepinephrine in cerebrospinal fluid in depression [33].…”

Section: Discussionmentioning

confidence: 99%

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Stainability and Fine Structure of Intracytoplasmic Inclusion Bodies in the Locus Ceruleus of Mouse

Katoh¹,

Sakai²,

Kaneko³

et al. 2013

JBBS

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The present study compared intracytoplasmic inclusion bodies in the locus ceruleus (LC) of mouse with protein bodies in the LC of human. Phosphotungstic acid-hematoxylin (PTAH) and the Mallory method were used as the anionic stains. The inclusion bodies and protein bodies, which stained with PTAH and the Mallory method in the brain, contain proteins that appear to belong to the same family. Although both inclusion and protein bodies were formed with the same arginine composition, their distribution in the brain was similar and similar physiological changes by morbidity were observed, and the fine structure of the inclusion bodies and protein bodies appeared to be different. The present findings suggest that mouse inclusion bodies and human protein bodies are different.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Stainability and Fine Structure of Intracytoplasmic Inclusion Bodies in the Locus Ceruleus of Mouse

Katoh¹,

Sakai²,

Kaneko³

et al. 2013

JBBS

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An aromatase‐associated cytoplasmic inclusion, the “stigmoid body,” in the rat brain: II. Ultrastructure (with a review of its history and nomenclature)

Shinoda

Nagano

Osawa

1993

J of Comparative Neurology

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The ultrastructure of aromatase-associated "stigmoid (dot-like) structures," which were detected in a previous study using light-microscopic immunohistochemistry (Shinoda et al.: J. Comp. Neurol. 322:360-376, '92), were examined in the rat medial preoptic region, bed nucleus of the stria terminalis, medial amygdaloid nucleus, and arcuate nucleus by pre- and post-embedding marking with a polyclonal antibody against human placental antigen X-P2 (hPAX-P2) for immuno-electron microscopic analysis. The immunoreactive stigmoid structure was identified as a distinct, non-membrane-bounded cytoplasmic inclusion (approximately 1-3 microns in diameter), which has a granulo-fuzzy texture with moderate-to-low electron density in non-immunostained preparations. It consists of at least four distinct granular and three distinct fibrillo-tubular elements forming a granulo-fibrillar conglomerate. This type of inclusions was formally termed the "stigmoid body" under the electron microscope. The stigmoid body is composed of the outer granulo-fibrillar and inner hyaloplasmic compartments. The immunoreactivity for hPAX-P2 is mainly localized to the former, especially to the low density granulo-fuzzy materials associated with the fibrillo-tubular elements. Identification of the ultrastructure of stigmoid body clarified their prevalence not only in the limbic and hypothalamic regions, but also in sex-steroid-sensitive peripheral tissues (e.g., peripheral sensory ganglia, ovary, testis) by consulting earlier electron-microscopic studies. Reviewing the history and nomenclature of this inclusion body, we reorganized the terminology of related neuronal cytoplasmic inclusions, the terms of which have often been confused, and discussed its functional significance on the basis of the present and previously accumulated data. In conclusion, we emphasized the importance of the stigmoid bodies in the sex-steroid-sensitive neural system because of their large size, high frequency, specific distribution in brains and peripheral tissues, effects of sex-steroids, and immunological and histochemical characteristics of the antibody marking the inclusion. The stigmoid bodies may provide a subcellular site for sex-steroid metabolism in their target tissues and play a critical role in cytosolic modulation of their actions (e.g., by aromatization) prior to their receptor binding.

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Visualization of three-dimensional stigmoid body in FFPE and ultrathin sections of mouse

et al. 2021

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Huntingtin-associated protein 1 (HAP1) is abundantly expressed in the neurons of the central nervous system and forms unique intracytoplasmic inclusions of unknown function called “stigmoid bodies” (STBs). Transmission electron microscopy has revealed that the STBs are aggregates of granules containing cavities with a diameter of 0.5–3 µm. Small STBs fuse to form larger STBs, the size of which is said to vary depending on the developmental growth stage and brain region. Light microscopy can only reveal that these STBs have similar circular shapes, due to its limited resolution. Therefore, light microscopy is only fit for the study of the STB distribution and quantitative changes. We, herein, suggest the adoption of correlative light and electron microscopy, that combines confocal laser scanning microscopy and scanning electron microscopy, as the method allowing us to identify the HAP1-positive STBs in formalin-fixed paraffin-embedded (FFPE) sections. This approach allows us to study the three-dimensional morphology of immunolabeled objects in histopathological specimens. The STBs in FFPE sections of murine hypothalami reflected the transmission electron microscopic images of Epon-embedded STBs, although we were not able to observe any organelle covering the STBs of the FFPE sections. Furthermore, we were able to reconstruct the three-dimensional structure of the STB and we identified it to be of spherical form, covered with mitochondria and rough endoplasmic reticulum, and bearing a cluster of cavities in the center. In the future, we might gain new insights by comparing the 3D structure of the STB between different neurons and under a variety of conditions.

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Quantitative changes of Holmes positive nucleolus-like inclusion bodies in the mouse locus coeruleus under various experimental conditions.

Cited by 4 publications

References 23 publications

Stainability and Fine Structure of Intracytoplasmic Inclusion Bodies in the Locus Ceruleus of Mouse

Stainability and Fine Structure of Intracytoplasmic Inclusion Bodies in the Locus Ceruleus of Mouse

An aromatase‐associated cytoplasmic inclusion, the “stigmoid body,” in the rat brain: II. Ultrastructure (with a review of its history and nomenclature)

Visualization of three-dimensional stigmoid body in FFPE and ultrathin sections of mouse

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