Quantitative Bioimaging to Investigate the Uptake of Mercury Species in <i>Drosophila melanogaster</i>

Niehoff, Ann-Christin; Bauer, Oliver; Kröger, Sabrina; Fingerhut, Stefanie; Schulz, Jacqueline; Meyer, Sören; Sperling, Michael; Jeibmann, Astrid; Schwerdtle, Tanja; Kärst, Uwe

doi:10.1021/acs.analchem.5b02500

Cited by 24 publications

(15 citation statements)

References 43 publications

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“…Fly studies have positively shown that targeting iron metabolism is sufficient to improve diseases conditions in models of FRDA (Navarro et al, 2015 ; Chen et al, 2016 ; Soriano et al, 2016 ), PD (Bonilla-Ramirez et al, 2011 ; Esposito et al, 2013 ; Zhu et al, 2016 ), or Alzheimer's disease (AD) (Rival et al, 2009 ; Liu et al, 2011 ; Ott et al, 2015 ). Besides iron, other functional studies have analyzed the regulation and homeostasis of other heavy metals (Maroni et al, 1986 ; Al-Momani and Massadeh, 2005 ; He et al, 2009 ; Ortiz et al, 2009 ; Meyer et al, 2014 ; Rovenko et al, 2014 ; Ternes et al, 2014 ; Chandra et al, 2015 ; Guan et al, 2015 ; Niehoff et al, 2015 ). Of special interest is the recent work of Anholt's lab (Zhou et al, 2017 ) analyzing the genetic networks involved in the resistance to Cadmium and Lead toxicity.…”

Section: Introductionmentioning

confidence: 99%

Copper and Zinc Homeostasis: Lessons from Drosophila melanogaster

Navarro

Schneuwly

2017

Front. Genet.

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Maintenance of metal homeostasis is crucial for many different enzymatic activities and in turn for cell function and survival. In addition, cells display detoxification and protective mechanisms against toxic accumulation of metals. Perturbation of any of these processes normally leads to cellular dysfunction and finally to cell death. In the last years, loss of metal regulation has been described as a common pathological feature in many human neurodegenerative diseases. However, in most cases, it is still a matter of debate whether such dyshomeostasis is a primary or a secondary downstream defect. In this review, we will summarize and critically evaluate the contribution of Drosophila to model human diseases that involve altered metabolism of metals or in which metal dyshomeostasis influence their pathobiology. As a prerequisite to use Drosophila as a model, we will recapitulate and describe the main features of core genes involved in copper and zinc metabolism that are conserved between mammals and flies. Drosophila presents some unique strengths to be at the forefront of neurobiological studies. The number of genetic tools, the possibility to easily test genetic interactions in vivo and the feasibility to perform unbiased genetic and pharmacological screens are some of the most prominent advantages of the fruitfly. In this work, we will pay special attention to the most important results reported in fly models to unveil the role of copper and zinc in cellular degeneration and their influence in the development and progression of human neurodegenerative pathologies such as Parkinson's disease, Alzheimer's disease, Huntington's disease, Friedreich's Ataxia or Menkes, and Wilson's diseases. Finally, we show how these studies performed in the fly have allowed to give further insight into the influence of copper and zinc in the molecular and cellular causes and consequences underlying these diseases as well as the discovery of new therapeutic strategies, which had not yet been described in other model systems.

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Section: Introductionmentioning

confidence: 99%

Copper and Zinc Homeostasis: Lessons from Drosophila melanogaster

Navarro

Schneuwly

2017

Front. Genet.

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“…One limitation of our study is the absence of data about the VCH and MeHg + absorption in flies. Concerning the distribution of MeHg + , large amounts of MeHg + were found in adult fly’s brain after oral exposure [114]. As for VCH, there are no data in the literature on the rate of absorption and distribution in living organisms.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster: biochemical and molecular analyses

Piccoli

Segatto

Oliveira

et al. 2019

BMC Pharmacol Toxicol

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BackgroundExposure to vinylcyclohexene (VCH) and methylmercury (MeHg+) can induce oxidative stress and gene modulation. Several studies have been evaluating the effects of VCH and MeHg+, but little is known about interactive effects between them. This work aimed to assess the exposure and co-exposure effects of MeHg+ and VCH on oxidative stress and gene modulation in Drosophila melanogaster.MethodsReactive species production, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities were evaluated after exposure and co-exposure to VCH (1 mM) and MeHg+ (0.2 mM) for one or three days in the head and body (thorax and abdomen) of flies. The expression of genes related to redox state and inflammatory response was evaluated after exposure and co-exposure to VCH and MeHg+ for three days.ResultsSurvival decreased only in flies co-exposed to VCH and MeHg+ for three days. All treatments increased total reactive species production after one day of exposure. However, no significant changes were observed in the head after three days of exposure. One day of exposure to VCH caused an increase in the head GST activity, whereas MeHg+ induced an increase after three days of exposure. Regarding the body, all treatments increased GST activity after one day of exposure, but only the flies exposed to MeHg+ presented an increase in GST activity after three days of exposure. Treatments did not alter AChE activity in the head. As for gene expression, there was a significant increase in the Relish transcription factor gene in the flies’ body, but Nrf2, Keap1, Jafrac1, TrxR1, and NF-κβ were not altered.ConclusionThe results suggest that exposure to VCH and MeHg+ induce oxidative stress and activation of an inflammatory response in fruit flies.

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“…The data were collated into a single image file using in-house developed software and visualised using FIJI. The limit of detection for mercury using LA-ICP-MS has been calculated to be 0.08 μg/g [23].…”

Section: Laser Ablation-inductively Coupled Plasma-mass Spectrometry mentioning

confidence: 99%

Elemental imaging shows mercury in cells of the human lateral and medial geniculate nuclei

et al. 2020

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Interference with the transmission of sensory signals along visual and auditory pathways has been implicated in the pathogenesis of hallucinations. The relay centres for vision (the lateral geniculate nucleus) and hearing (the medial geniculate nucleus) appear to be susceptible to the uptake of circulating mercury. We therefore investigated the distribution of mercury in cells of both these geniculate nuclei. Materials and methods Paraffin-embedded tissue sections containing the lateral geniculate nucleus were obtained from 50 adults (age range 20-104 years) who at autopsy had a variety of clinicopathological conditions, including neurological and psychiatric disorders. The medial geniculate nucleus was present in seven sections. Sections were stained for mercury using autometallography. Laser ablation-inductively coupled plasma-mass spectrometry was used to confirm the presence of mercury. Results Ten people had mercury in cells of the lateral geniculate nucleus, and in the medial geniculate nucleus of three of these. Medical diagnoses in these individuals were: none (3), Parkinson disease (3), and one each of depression, bipolar disorder, multiple sclerosis, and mercury self-injection. Mercury was distributed in different groups of geniculate capillary endothelial cells, neurons, oligodendrocytes, and astrocytes. Mass spectrometry confirmed the presence of mercury. Conclusion Mercury is present in different combinations of cell types in the lateral and medial geniculate nuclei in a proportion of people from varied backgrounds. This raises the possibility that mercury-induced impairment of the function of the geniculate nuclei could play a part in the

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Quantitative Bioimaging to Investigate the Uptake of Mercury Species in Drosophila melanogaster

Cited by 24 publications

References 43 publications

Copper and Zinc Homeostasis: Lessons from Drosophila melanogaster

Copper and Zinc Homeostasis: Lessons from Drosophila melanogaster

Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster: biochemical and molecular analyses

Elemental imaging shows mercury in cells of the human lateral and medial geniculate nuclei

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