Quantitative bioimaging of platinum group elements in tumor spheroids

Niehoff, Ann-Christin; Grünebaum, Jonas; Moosmann, Aline; Mulac, Dennis; Söbbing, Judith; Niehaus, Rebecca; Buchholz, Rebecca; Kröger, Sabrina; Wiehe, Arno; Wagner, Sylvia; Sperling, Michael; Briesen, Hagen von; Langer, Klaus; Kärst, Uwe

doi:10.1016/j.aca.2016.07.021

Cited by 33 publications

(28 citation statements)

References 25 publications

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“…They performed an on tissue chemical derivatization using diethyldithiocarbamate to overcome platinum's low ionization efficiency in MALDI MSI . They observed an accumulation of platinum in the periphery and the core of the MCTS, as has been observed previously by LA‐ICP‐MS . However they were also able to identify an inactive methionine bound metabolite localized to the core of the MCTS showing that MALDI can provide additional information about drug penetration into MCTS with appropriate sample preparation.…”

Section: Analysis Techniques For Investigating Mctsmentioning

confidence: 58%

“…They suggest that the localization of the inactive metabolite to the necrotic core is a product of increased detoxification in this area or an expulsion of this product from the quiescent layer. This quiescent layer is consistently devoid of platinum based chemotherapeutics, despite complete penetration of the drugs, suggesting that these cells possess detoxification or drug efflux mechanisms.…”

Section: Analysis Techniques For Investigating Mctsmentioning

confidence: 99%

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Mass Spectrometry Analyses of Multicellular Tumor Spheroids

Acland

Mittal

Lokman

et al. 2018

Proteomics Clinical Apps

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Multicellular tumor spheroids (MCTS) are a powerful biological in vitro model, which closely mimics the 3D structure of primary avascularized tumors. Mass spectrometry (MS) has established itself as a powerful analytical tool, not only to better understand and describe the complex structure of MCTS, but also to monitor their response to cancer therapeutics. The first part of this review focuses on traditional mass spectrometry approaches with an emphasis on elucidating the molecular characteristics of these structures. Then the mass spectrometry imaging (MSI) approaches used to obtain spatially defined information from MCTS is described. Finally the analysis of primary spheroids, such as those present in ovarian cancer, and the great potential that mass spectrometry analysis of these structures has for improved understanding of cancer progression and for personalized in vitro therapeutic testing is discussed.

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Section: Analysis Techniques For Investigating Mctsmentioning

confidence: 58%

Section: Analysis Techniques For Investigating Mctsmentioning

confidence: 99%

Mass Spectrometry Analyses of Multicellular Tumor Spheroids

Acland

Mittal

Lokman

et al. 2018

Proteomics Clinical Apps

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“…Gelatin has good solubility, which makes it possible to mitigate the problems associated with homogenates from raw animal tissues. [22][23][24][25] However, the obvious phenomenon of elementdependent heterogeneities is found in both gelatin and agarose-gel films. This is caused by the so-called "coffee stain" (visible in the elemental image since higher concentrations are found at the edges) and the "Marangoni" (visible as higher elemental concentrations in the center) effect.…”

Section: Introductionmentioning

confidence: 99%

Quantitation And Imaging Analysis Of Biological Samples By LA-ICP-MS

Guo¹

2021

At.Spectrosc.

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Analysis of the elemental abundance and distribution in biological tissues by laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS) adds to clarifying the basic questions of metabolic research and enables bioaccumulation and bioavailability studies in ecological and toxicological risk assessment. In this work, a method based on matrix-matched gelatin calibration LA-ICP-MS for the determination of essential and toxic elements in biological samples was developed. By using a moldprepared procedure, the elemental inhomogeneity distributions in the synthesized gelatin gels were improved, which was verified by using the 3D (surface-and depth-mapping) LA-ICP-MS protocols. The limits of detection (LODs) ranged from 0.014 μg g −1 (Ba) to 48 μg g −1 (K). The results of the analysis of biological reference materials (RMs) were in good agreement with the certified values. Furthermore, a reliable bio-mapping LA-ICP-MS method is proposed for studying the metabolism of heavy metals in rat liver injected with CdS/PbS quantum dots. Our results show that the high concentration of Pb and Cd co-exist (positive correlation as high as 78%) in hepatocytes and sinusoids, which indicates that the PbS/CdS quantum dots are not dissociated into toxic heavy metal ions (i.e., Pb 2+ , Cd 2+ ) in the metabolic process of the liver.

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“…The tedious preparation and characterisation, and the susceptibility towards systematic errors has encouraged investigations of other standard materials which are easier to manufacture, offer improved traceability and show less variability. Hydrocolloid gel-based materials such as gelatine-based standards are becoming increasingly popular for the calibration of elements in biological tissues as similarities in their composition mitigate the problems associated with homogenates from raw animal tissues 15,[24][25][26] . Three major methods for the fabrication of gelatine standards are described in literature: pipetted films 27 , spotted droplets 24 and cryosections 28 .…”

Section: Introductionmentioning

confidence: 99%