Quantitative bestimmung von L(+)-Milchsäure mit Milchsäuredehydrogenase

Horn, H.; Bruns, Friedrich

doi:10.1016/0006-3002(56)90023-3

Cited by 202 publications

(34 citation statements)

References 5 publications

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“…Blood samples were analysed for nitrous oxide, oxygen, and carbon dioxide in a manometric Van Slyke apparatus. Blood sugar was measured by the glucose-oxidase method (Huggett & Nixon, 1957), lactate and pyruvate enzymatically (Horn & Bruns, 1956), and non-esterified fatty acid (NEFA) by the method of Trout, Estes & Friedberg (1960). Insulin-like activity was measured by a modification of the method of Morgan & Lazarow (1963).…”

Section: Methodsmentioning

confidence: 99%

THE EFFECT OF PROSTAGLANDIN E₁ UPON THE GENERAL AND CORONARY HAEMODYNAMICS AND METABOLISM OF THE INTACT DOG

Maxwell

1967

British Journal of Pharmacology and Chemotherapy

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The prostaglandins are unsaturated hydroxy-carboxylic acids, which for many years have been known to have vasodepressor and smooth-muscle stimulant properties (von Euler, 1937); one of these, prostaglandin E1 (PGE1), has been synthesized (Bergstrom, Ryhage, Samuelsson & Sjovall, 1962); the natural PGE1 has been shown to reduce blood pressure and increase heart rate in man and animals (Bergstrom, Carlson, Ekelund & Oro, 1965; Bergstrom, Carlson & Orc, 1966). There is, however, no integrated study of the effect of this substance on general and cardiac haemodynamics and metabolism; accordingly, such a study was made, and the results are now presented. METHODSTen apparently healthy dogs weighing from 13-17 kg were given morphine sulphate (3 mg/kg, subcutaneously). After 1 hr, a mixture of pentobarbitone (12.5 mg/kg), allobarbitone (12.5 mg/kg), monoethylthiourea (50 mg/kg) and urethane (50 mg/kg) was injected intravenously. A cuffed tube was placed in the trachea.The external jugular veins were isolated and standard cardiac catheters placed therein; under fluoroscopic control one catheter tip was placed in a main branch of the pulmonary artery and another in the coronary sinus. The position of the latter was verified by its characteristic x-ray silhouette, and by aspiration through it of blood which was much reduced in oxygen content compared to a sample from the pulmonary artery. A Cournand needle was inserted percutaneously into each femoral artery. Catheters and needles were connected to a manifold system which allowed pressures to be measured and blood samples taken.One hour after administration of the intravenous anaesthetic, cardiac output was calculated by the Fick principle from measurements of gas exchange and blood gas concentrations. Expired air was collected from the endotracheal tube into a Tissot spirometer, whence the minute volume was determined. The gas was collected for 5 min and appropriate blood samples were taken at the midpoint of the collection.Immediately after the determination of cardiac output, coronary flow was measured by the nitrous oxide saturation method. During this period the animal breathed a gas mixture containing nitrous oxide (150o), oxygen (21%) and nitrogen (64%). Mean coronary flow was estimated over a 10 min period.

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Section: Methodsmentioning

confidence: 99%

THE EFFECT OF PROSTAGLANDIN E₁ UPON THE GENERAL AND CORONARY HAEMODYNAMICS AND METABOLISM OF THE INTACT DOG

Maxwell

1967

British Journal of Pharmacology and Chemotherapy

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“…Immediately after the determination of cardiac output, coronary flow was measured by the nitrous oxide saturation method; during this period the animal breathed a gas mixture containing nitrous oxide (15%), oxygen (21%) and nitrogen (64% (Horn & Bruna, 1956), and sodium and potassium in a flame-photometer. After 30 min from the control measurements of cardiac output and coronary flow, the effect of synthetic eledoisin was examined.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Synthetic Eledoisin on the Systemic and Coronary Haemodynamics and Metabolism of the Intact Dog

Maxwell

1964

British Journal of Pharmacology and Chemotherapy

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Cardiac output and coronary blood flow were measured before and after intravenous injection of eledoisin into anaesthetized dogs. The following changes, statistically significant at the 5% level, were found: increase of heart rate, and decreases in systemic and pulmonary arterial blood pressure, cardiac output, stroke volume, and in right and left ventricular work. Coronary flow decreased, cardiac oxygen extraction increased, and cardiac efficiency decreased. Many of these effects are similar to those caused by bacterial endotoxin.Eledoisin, a polypeptide derived from the salivary glands of a Mediterranean octopus (Erspamer, 1949), causes a fall of arterial pressure in mammals. This compound has been synthesized (Sandrin & Boissonnas, 1962). Eledoisin has been administered to unanaesthetized dogs (Olmsted & Page, 1962) and to man (Sicuteri, Fanciullacci, Franchi & Michelacci, 1963) and is said to dilate the coronary arteries (Bergamaschi & Glasser, 1963). However, no detailed examination of its effect upon the systemic and coronary vascular system has been made. This paper presents the results of experiments in which the effects of synthetic eledoisin were investigated in-dogs. METHODSEleven apparently healthy dogs weighing from 9 to 25 kg were given morphine sulphate (3 mg/kg, subcutaneously). After 1 hr, a mixture of pentobarbitone (12.5 mg/kg), allobarbitone (12.5 mg/kg), monoethylthiourea (50 mg/kg) and urethane (50 mg/kg) was injected intravenously. A cuffed tube was placed in the trachea.The superficial neck veins were isolated and standard cardiac catheters placed therein under fluoroscopic control, one catheter tip was placed in a main branch of the pulmonary artery, and another in the coronary sinus. The position of the latter was verified by its characteristic X-ray silhouette, and by aspiration through it of blood which was much reduced in oxygen content compared to a sample from the pulmonary artery. A Cournand needle was inserted percutaneously into each femoral artery. Catheters and needles were connected to a manifold system which allowed pressures to be measured, and blood samples taken.One hour after administration of the intravenous anaesthetic, cardiac output was calculated by the Fick principle from measurements of gas exchange and blood gas concentrations. Expired air was collected from the endotracheal tube into a Tissot spirometer, whence the minute volume was determined. The gas was collected for 5 min, and appropriate blood samples were taken at the midpoint of the collection.

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“…LA was measured by the method of Horn and Bruns (1956), adapted to the microscale as previously described (Burgess and Sylven, 1962). In this method the sample was incubated together with lactic dehydrogenase and DPN at pH 10*5 for 120 minutes, after which the total amount of DPNH formed was measured spectrophotometrically.…”

Section: Methodsmentioning

confidence: 99%

Changes in Glucose and Lactate Content of Ascites Fluid and Blood Plasma During Growth and Decay of the ELD Ascites Tumour

Burgess¹,

Sylvén²

1962

Br J Cancer

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WHEN considering the nutritional and transport facilities of tumours one is struck by the diverging conditions developing in ascites and solid tumours with time. Early transplants of both solid and ascites tumours seem close enough to the vascular svstem of the host. In the case of solid tumours, with increasing age and size, vascular occlusion and deranged interstitial transport facilities (Goldacre andSylven, 1959, 1962) lead to starvation and cell death in central tumour regions. In ascites tumours the regressive changes during later stages have for various reasons not been extensively recognized. However, Lucke and Berwick (1954) found a decrease in the number of free ascites cells in the later stages of growth of the Ehrlich-Landschutz (ELD) ascites tumour. Hauschka et al. (1957) reported no such fall in cell number when describing the growth of ELD ascites, but Revesz and Klein (1954) found a decrease in ascites lymphomas.The present communication will draw attention to the spontaneous cellular decay and decrease in tumour cell numbers occurring during later stages of ELD ascites growth some time before the animals succumb. In addition, it became of interest to make serial assays of the content of glucose and lactate (LA) in blood plasma and ascites fluid in order to obtain rough information as to the nutritional supply and magnitude of glycolysis, respectively, along the growth curve. The latter parameters may be compared with previous data on the glucose and LA concentrations in the interstitial fluid from different regions of solid malignant mouse tumours recently assayed (Burgess and Sylven, 1962). MATERIAL AND METHODSMice and tumour strains.-The experiments were performed using only C3H mice or F1 hybrids of C3H x DBA mice. The hyperdiploid Ehrlich-Landschutz (ELD) ascites tumour was employed.Results obtained from 4 experimental series of animals are reported. Each series consisted of 50 animals inoculated with about 25 x 106 cells obtained from mice bearing 10 day ascites. This was an excess of mice, as some died during the course of the experiment. The times when these died were noted.Nutritional conditions. All mice were given food and water ad libitum. It was the aim of this work to study the growth of the ascites tumour under ordinary laboratory conditions. The results will show that the animals were in a state

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Quantitative bestimmung von L(+)-Milchsäure mit Milchsäuredehydrogenase

Cited by 202 publications

References 5 publications

THE EFFECT OF PROSTAGLANDIN E₁ UPON THE GENERAL AND CORONARY HAEMODYNAMICS AND METABOLISM OF THE INTACT DOG

THE EFFECT OF PROSTAGLANDIN E₁ UPON THE GENERAL AND CORONARY HAEMODYNAMICS AND METABOLISM OF THE INTACT DOG

The Effects of Synthetic Eledoisin on the Systemic and Coronary Haemodynamics and Metabolism of the Intact Dog

Changes in Glucose and Lactate Content of Ascites Fluid and Blood Plasma During Growth and Decay of the ELD Ascites Tumour

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Quantitative bestimmung von L(+)-Milchsäure mit Milchsäuredehydrogenase

Cited by 202 publications

References 5 publications

THE EFFECT OF PROSTAGLANDIN E1 UPON THE GENERAL AND CORONARY HAEMODYNAMICS AND METABOLISM OF THE INTACT DOG

THE EFFECT OF PROSTAGLANDIN E1 UPON THE GENERAL AND CORONARY HAEMODYNAMICS AND METABOLISM OF THE INTACT DOG

The Effects of Synthetic Eledoisin on the Systemic and Coronary Haemodynamics and Metabolism of the Intact Dog

Changes in Glucose and Lactate Content of Ascites Fluid and Blood Plasma During Growth and Decay of the ELD Ascites Tumour

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THE EFFECT OF PROSTAGLANDIN E₁ UPON THE GENERAL AND CORONARY HAEMODYNAMICS AND METABOLISM OF THE INTACT DOG

THE EFFECT OF PROSTAGLANDIN E₁ UPON THE GENERAL AND CORONARY HAEMODYNAMICS AND METABOLISM OF THE INTACT DOG