Quantitative Bestimmung des Gesamtlipoidgehaltes von Naturstoffen

Reichert, Rolf

doi:10.1002/hlca.194402701121

Cited by 34 publications

(4 citation statements)

References 4 publications

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“…RNA was determined by 12 0 7 (b) The chemical analysis of a purified chromatophore preparation was compared with a crude particulate preparation. The determinations were carried out as follows: protein, by the method of Lowry et al (1951); lipid, extracted and determined by the method of Few (1955) after refluxing with methanol to break lipoprotein complexes (Reichert, 1944); total water-soluble carbohydrate, by the method of Loewus (1952); bacteriochlorophyll and carotenoids, by the method of Cohen-Bazire et al (1957); total nucleic acid, by the method of Gale & Folkes (1954); total P and lipid P, by the method of Sumner (1944) after digestion of whole chromatophore or lipid preparations in the conc. sulphuric acid-60% (v/v) perchloric acid (3:2, vlv) mixture of Hanes & Isherwood (1949 0-3-0.9 Total P (iumole/mg.…”

Section: Protein and Pigment Synthesismentioning

confidence: 99%

The association of protein synthesis with formation of pigments in some photosynthetic bacteria

Bull¹,

Lascelles²

1963

Biochemical Journal

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NUCLEIC ACID METABOLISM IN DIVIDING LIVER 15 are not so marked as those occurring in young and foetal liver. This is to be expected by analogy with thymine catabolism. The effect of X-radiation on thymine catabolism in regenerating liver shows that, under conditions in which the formation of thymidine kinase is delayed, a delay in the depression of thymine catabolism also occurs. It is possible that the depression in thymine catabolism may be influenced by the appearance of thymidine kinase which occurs at about 18 hr. after partial hepatectomy (Bollum & Potter, 1959). When the formation of thymidine kinase is inhibited by X-radiation, the depression in thymine catabolism is delayed. SUMMARY 1. The activities of enzymes involved in nucleic acid metabolism in foetal, young and regenerating rat liver were compared with those in normal rat liver. 2. Thymidine phosphorylase and uridine phosphorylase were less active in foetal and young liver than in adult liver. Thymidine-phosphorylase activity was also reduced in regenerating liver. 3. Thymidine was incorporated into DNA in vitro more rapidly in the liver of foetal and young rats than in adult rats. 4. Dihydro-orotase activity was maximal in the liver at birth when it was twice as active as in the adult. 5. Total-body X-radiation (400 r.), when given to rats 6 hr. after partial hepatectomy, delayed the decrease in thymine catabolism.

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Section: Protein and Pigment Synthesismentioning

confidence: 99%

The association of protein synthesis with formation of pigments in some photosynthetic bacteria

Bull¹,

Lascelles²

1963

Biochemical Journal

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“…Estimations of lipids. This procedure, which replaces methods involving extraction of the dried product in a Soxhlet with ether or light petroleum, is a combination of the methods described by Nielson & Bohart (1944) and Reichert (1944).…”

Section: Methodsmentioning

confidence: 99%

The fermentation process in tea manufacture. 12. The origin of carbon dioxide

Roberts

Wood

1952

Biochemical Journal

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“…In preparation for the alkali and acid extraction, lipid was exhaustively extracted from two 5 g. samples of dried organism by repeatedly heating under reflux with 95 yo aqueous methanol for 1 hr., evaporating to 976 P. Mitchell and J . Moyle dryness and extracting in a Soxhlet apparatus with sodium-dried ether (Reichert, 1944).…”

Section: Isherwood (1949)mentioning

confidence: 99%

Isolation of Hydrolytic Products of a Glycerophospho-compound from Micrococcus pyogenes

Mitchell

Moyle

1951

Journal of General Microbiology

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SUMMARY:The ribonucleic acid fraction of Micrococcus pyogenes var. aureus (strain Duncan), separated by the method of Schmidt & Thannhauser (1945), contains organic phosphate in excess of that which can be accounted for by a polynucleotide nucleic acid structure. This 'excess phosphate' can be separated from the other phospho-compounds after fractional extraction of M . pyogenes in dilute acid or in dilute alkali. Four polyolphosphate compounds occur in the extracts. The main components are a-and /3-glycerophosphate, the two other polyolphosphates (giving water-insoluble barium salts ; separable chromatographically) occurring in smaller amount. The yields of glycerophosphate show that at least 75 yo of the 'excess phosphate ' is present in an easily hydrolysed glycerophospho-compound which may also be the origin of the unidentified polyolphosphates. The glycerophospho-compound of M . pyogenes accounts for more than one-quarter of the total organic phosphate of the organism. The presence of 'excess phosphate' in a number of Gram-positive organisms suggests that a glycerophospho-compound similar to that of M. pyogenes may be of general occurrence in Gram-positive bacteria and in yeasts.

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Quantitative Bestimmung des Gesamtlipoidgehaltes von Naturstoffen

Cited by 34 publications

References 4 publications

The association of protein synthesis with formation of pigments in some photosynthetic bacteria

The association of protein synthesis with formation of pigments in some photosynthetic bacteria

The fermentation process in tea manufacture. 12. The origin of carbon dioxide

Isolation of Hydrolytic Products of a Glycerophospho-compound from Micrococcus pyogenes

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