Quantitative autoradiographic study on the RNA synthesis in the neurons after mercury intoxication

Role of platinum DNA damage‐induced transcriptional inhibition in chemotherapy‐induced neuronal atrophy and peripheral neurotoxicity

“…; Chang et al . ; Carmichael and Cavanagh ; Wells and Vaidya ), there have been few previous studies of the global transcriptional activity of DRG neurons. We showed high, but wide ranging, global levels of transcription in individual DRG neurons that correlated with their cell body size.…”

“…In this study, we used a click chemistry EU RNA incorporation fluorescence labelling assay (Jao and Salic 2008) to visualize and measure global transcriptional levels in individual DRG neurons in vitro. Since early autoradiography studies (Sensenbrenner et al 1970;Chang et al 1972;Carmichael and Cavanagh 1976;Wells and Vaidya 1994), there have been few previous studies of the global transcriptional activity of DRG neurons. We showed high, but wide ranging, global levels of transcription in individual DRG neurons that correlated with their cell body size.…”

“…; Chang et al . ; Carmichael and Cavanagh ; Wells and Vaidya ). Reduced DRG neuronal cell body size is a morphological hallmark of platinum‐induced peripheral neurotoxicity, demonstrated in many previous in vivo studies of human and animal DRG tissue (Tomiwa et al .…”

“…DRG neurons have large cell bodies, prominent nucleoli and distant axonal connections (Scott 1992;Sghirlanzoni et al 2005;Berciano et al 2007). Studies of other cell types suggest that cell size is dependent on their global transcriptional activity as a generalizable biological relationship (Marguerat and B€ ahler 2012), but there have been few studies of the global transcriptional activity of DRG neurons since early autoradiography studies (Sensenbrenner et al 1970;Chang et al 1972;Carmichael and Cavanagh 1976;Wells and Vaidya 1994). Reduced DRG neuronal cell body size is a morphological hallmark of platinum-induced peripheral neurotoxicity, demonstrated in many previous in vivo studies of human and animal DRG tissue (Tomiwa et al 1986;Cavaletti et al 1992Cavaletti et al , 2001Holmes et al 1998;Krarup-Hansen et al 1999;Jamieson et al 2005;Renn et al 2011), but exactly how platinum drugs alter neuronal size is unclear.…”

In Vitro Toxicity Testing of Environmental Agents

“…These include turnover (Friberg and Vostal, 1972); histopathology (Berlin and Ulberg, 1963;Takeuchi, 1972;Berthoud et al, 1976;Chang and Hartman, 1972); ultrastructural changes (Chang andHartmann, 1972a, 1972b;Eto and Takeuchi, 1977;Chang, 1977); localization in brain cells (Berlin and Ulberg, 1963;Takeachi, 1972;Cassano et al, 1969;Garman et al, 1975;Miyakawa et a1., 1970;Vallee and Ulner, 1972); neurotransmitter uptake, release (Mannalis and Cooper, 1975;Landeka and Kostial, 1975;Bondy et a1., 1979), and contents (Hrdina et a1., 1976;Taylor and Distefano, 1976); biotransformation (Friberg and Vostal, 1972;; cellular distribution (Berlin and Ulberg, 1963;Berthoud et al, 1976;Chang and Hartmann, 1972;Cassano et al, 1969;Garman et al, 1975); changes in enzyme activity (Vallee and Ulner, 1972;Brubaker et al, 1973;Neathery and Miller, 1973); nucleic acid and protein synthesis (Brubaker et al, 1973;Neathery and Miller, 1973;Chang et al, 1972;Cheung and Verity, 1977); binding with protein complexes (Vallee and Ulner, 1972;; respiration (Fox et al, 1975;Cheung and Verity, 1978); and electrophysiological studies of peripheral nerves (Mannalis and Cooper, 1975;…”