Quantitative autoradiographic distribution of NPFF1 neuropeptide FF receptor in the rat brain and comparison with NPFF2 receptor by using [125I]YVP and [125I]EYF as selective radioligands

Physical Association between Neuropeptide FF and μ-Opioid Receptors as a Possible Molecular Basis for Anti-opioid Activity

“…NPFF-related peptides were synthesized with an automated synthesizer (Applied Biosystems model 433A). DAMGO (Tyr-D-Ala-Gly-NMe-Phe-gly-ol) was purchased from Bachem (France), and levorphanol was from Hoffmann Laroche (France (29). Radioiodinated peptides were stored at 4°C in the presence of 0.1% bovine serum albumin (Sigma, France).…”

The role of RFamide peptides in feeding

“…In line with its role in nociception, NPFF and NPFF2 are expressed in dorsal horn of the spinal cord (Panula et al 1996, Vilim et al 1999, Gouarderes et al 2002. It is important to point out that pronounced inter-species and inter-strain differences have been reported for the localisation of NPFF and its receptors (Boersma et al 1993, Gouarderes et al 2002, 2004b.…”

“…However, the lack of mRNA expression in this region (Vilim et al 1999) suggests that the immunostaining may be due to cross-reactivity of antibodies with other related RFamide peptides (such as RFRP) which have been reported in the region . While NPFF immunoreactive fibres have a fairly wide distribution in the rat brain, major targets include the PVN, the lPBN and the VLM, as all the three areas exhibit dense NPFF-containing nerve fibres, NPFF receptor expression and NPFF-binding activity (Kivipelto et al 1989, Boersma et al 1993, Vilim et al 1999, Liu et al 2001, Gouarderes et al 2002, 2004a, Goncharuk et al 2006. PVN neurons have also been shown to be electrically responsive to NPFF and express the immediate early gene, c-fos, following intracerebroventricular (ICV) injection of the peptide (Jhamandas & MacTavish 2003).…”

“…However, it has been proposed that BIBP3226 mimics the RF residues of NPFF-related peptides (neuropeptide Y terminates inRYamide) and binding of the compound to RFamide receptors has been demonstrated previously (Bonini et al 2000, Gouarderes et al 2002, Fang et al 2006, Vyas et al 2006. In addition to pharmacological evidence showing a role for QRFP and 26RFa in food intake, the expression of mRNA for the QRFP/26RFa precursor, measured using real-time reverse transcription (RT)-PCR of dissected tissues, is elevated in the 'hypothalamic' region of fasted mice, as well as in obese ob/ob and db/db mice (Takayasu et al 2006).…”

Identification of proNeuropeptide FFA peptides processed in neuronal and non‐neuronal cells and in nervous tissue

“…Pharmacological characterization of these receptors in recombinant cell lines showed a better selectivity of peptides deduced from proNPFF A sequence for NPFF 2 receptors binding, whereas proN-PFF B -derived peptides displayed a greater affinity for NPFF 1 receptors [26]. Autoradiographic studies performed on rat CNS with highly selective radioligands revealed the localization of both receptors in central nervous areas implicated in pain transmission [27]. The existence of two peptidergic systems of neurotransmission, mediated through NPFF 1 and NPFF 2 receptor stimulation by peptides generated by proNPFF B and proNPFF A processing, respectively, could explain the complex pharmacological effects of NPFF.…”