Quantitative Assessment of the Predators of
Nezara viridula
Eggs and Nymphs within a Soybean Agroecosystem Using an Elisa
1

Ragsdale, David W.; Larson, A. D.; Newsom, L. D.

doi:10.1093/ee/10.3.402

Cited by 64 publications

(33 citation statements)

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“…Ragsdale, Larson, and Newsom (1981) identified 27 species of insects and spiders that attacked eggs and nymphs of N. viridula in soybean, but the relative contributions of the species were not definitively ascertained. Others found that ants can be significant predators of 1418 D.M.…”

Section: Introductionmentioning

confidence: 99%

Crop-specific mortality of southern green stink bug eggs in Bt- and non-Bt cotton, soybean and peanut

Olson¹,

Ruberson

2012

Biocontrol Science and Technology

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Increasing insecticide use against stink bugs erodes benefits accrued to cotton production in the southeastern USA by eradication of the boll weevil and widespread use of Bt-transgenic cotton. Biological control programs designed to mitigate the impact of these pests would be beneficial; therefore, we sought to identify and assess naturally occurring biological control agents of stink bug eggs in cotton, peanut and soybeans, three important crops and stink bug hosts in the region. Two studies were conducted utilising digital imaging of sentinel egg masses to capture enemies present and sequential imaging over time to characterise the type and amount of damage inflicted on the egg masses. The first study utilised plots of soybean, Bt cotton, non-Bt cotton and peanut. The second study focused on ant predation in cotton by excluding fire ants from half of the plots. Sentinel egg masses of the southern green stink bug were placed in all plots at weekly intervals and were photographed at placement and at intervals over 72 h. Surviving egg masses were collected and held in the laboratory to assess parasitism in the first study. Predation of eggs was high in peanut (74Á86%) and soybean (39Á65%), and lower in Bt (26Á34%) and non-Bt (4Á21%) cotton over the two years. Parasitism was low overall (0.17Á19.0%). Most egg predation in cotton and peanut was attributable to fire ants, whereas long-horned grasshoppers dominated in soybean. Exclusion of fire ants in cotton significantly reduced egg mortality, indicating their predominance as mortality agents in cotton.

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Section: Introductionmentioning

confidence: 99%

Crop-specific mortality of southern green stink bug eggs in Bt- and non-Bt cotton, soybean and peanut

Olson¹,

Ruberson

2012

Biocontrol Science and Technology

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“…Dempster, 1960;Sutton, 1970;Service, 1973;Tod, 1973;Ashby, 1974;Vickerman & Sunderland, 1975;Lund & Turpin, 1977;Allen & Hagley, 1982;Dennison & Hodkinson, 1983;Doane et al, 1985) and ELISAs (e.g. Ragsdale et al, 1981;Crook & Sunderland, 1984;Schoof et al, 1986;Walter et al, 1986;Sopp, 1987;Cameron & Reeves, 1990). However, although this approach is possibly the most practical solution where small prey are involved, such as aphids or mites, it does mean that the antisera will usually contain a large number of antibody species.…”

Section: Introductionmentioning

confidence: 99%

The development and characterization of an anti‐haemolymph antiserum for the detection of mollusc remains within carabid beetles

Symondson

Liddell

1993

Biocontrol Science and Technology

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Molluscan haemolymph was evaluated as an antigen for the detection, by enzyme-linked immunosorbent assay (ELISA), of mollusc proteins within the crop contents of carabid beetles, using an anti-haemolymph antiserum. The value of using a narrow range of prey-specific immunogens, rather than whole-body macerates, was discussed. Predators and potential prey were tested by a quantitative indirect ELISA, with separate evaluation of antigen reactions with non-specific and specific antibodies. The assay proved to be capable of detecting less than 1 ng of haemolymph protein and the antiserum reacted strongly with all molluscs tested. Initial cross-reactions with earthworms were effectively eliminated by absorption. A range of invertebrates was tested, and a significance level established relative to the invertebrates giving the strongest cross-reaction, which proved to be wolf spiders, Pardosa sp. Harvestmen (Opiliones), that initially gave strong reactions to the antiserum, were shown to have fed upon molluscs. Laboratory tests on the crop contents of slug-fed beetles demonstrated clear detection for at least 4 days after feeding.

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“…This is especially true of predators with sucking mouthparts. Some recent reports have implicated Geocoris punctipes (Say) as an important predator of eggs of several species of agricultural pests (Crocker & Whitcomb, 1980, Ragsdale et aL, 1981. The present study was undertaken to examine the quantitative aspects of nutrition in the predaceous hemipteran G. punctipes and includes measurements of food consumption, growth, metabolic and digestive efficiencies and metabolic rates of this predator fed eggs of the lepidopterous pest, the tobacco budworm, Heliothis virescens (Fabricius).…”

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confidence: 99%

Food consumption, food utilization, and metabolic rates ofGeocoris punctipes [Het.: Lygaeidae] fedHeliothis virescens [Lep.: Noctuidae] eggs

Cohen

1984

Entomophaga

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Food consumption and utilization were measured in Geocoris punctipes (Say) from 1st instar nymphs to adults that were fed eggs of Heliothis virescens (Fabricius). Growth in both sexes was similar until the final portion of the last nymphal period, when female growth rose sharply over that of males. Food absorption efficiency was 95.2 % ; growth efficiency (ratio of weight ingested) was 52.9 %, and metabolic efficiency was 55.6 %. RQ was 0.93, and oxygen consumption rates were 0.84, 1.37, and 1.80/.tl O2 mg body weight h -1 at 24 ~ 30 ~ and 35 ~ respectively. Qlo for O2 consumption was 2.14 (25-35 ~ Since the appearance of Waldbauer's (1968) and Gordon's (1968) definitive treatments of quantitative nutrition of insects, numerous studies have appeared reporting consumption and utilization values in many kinds of insects and several kinds of food. However, Slansky & Seriber's (1982) review of this subject reveals that there has been remarkably little attention to food consumption and utilization in predaceous terrestrial insects. This is especially true of predators with sucking mouthparts. Some recent reports have implicated Geocoris punctipes (Say) as an important predator of eggs of several species of agricultural pests (Crocker & Whitcomb, 1980, Ragsdale et aL, 1981. The present study was undertaken to examine the quantitative aspects of nutrition in the predaceous hemipteran G. punctipes and includes measurements of food consumption, growth, metabolic and digestive efficiencies and metabolic rates of this predator fed eggs of the lepidopterous pest, the tobacco budworm, Heliothis virescens (Fabricius). MATERIALS AND METHODSGeocoris punctipes used to initiate cultures for these tests were collected from 2 alfalfa farms in the Avra Valley ca. 32 km northwest of Tucson, AZ. They were kept in ca. 4 L card-(1) Mention of a commercial product does not constitute a recommendation by the U.S. Department of Agriculture.

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Quantitative Assessment of the Predators of Nezara viridula Eggs and Nymphs within a Soybean Agroecosystem Using an Elisa 1

Cited by 64 publications

References 0 publications

Crop-specific mortality of southern green stink bug eggs in Bt- and non-Bt cotton, soybean and peanut

Crop-specific mortality of southern green stink bug eggs in Bt- and non-Bt cotton, soybean and peanut

The development and characterization of an anti‐haemolymph antiserum for the detection of mollusc remains within carabid beetles

Food consumption, food utilization, and metabolic rates ofGeocoris punctipes [Het.: Lygaeidae] fedHeliothis virescens [Lep.: Noctuidae] eggs

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