Quantitative Assessment of the Chloroplast Lipidome

Gros, Valérie; Jouhet, Juliette

doi:10.1007/978-1-4939-8654-5_16

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…In these conditions, the produced ions were mainly present as H - , H + , NH 4 + , or Na + adducts. Lipids were identified by MS analysis with their precursor ion or by neutral loss analyses after low energy collision-induced dissociation, as previously described (Gros and Jouhet, 2018).…”

Section: Methodsmentioning

confidence: 99%

Lipid Phases and Cell Geometry During the Cell Cycle of Streptococcus pneumoniae

et al. 2019

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The coexistence of different lipid phases is well-known in vitro, but evidence for their presence and function in cellular membranes remains scarce. Using a combination of fluorescent lipid probes, we observe segregation of domains that suggests the coexistence of liquid and gel phases in the membrane of Streptococcus pneumoniae, where they are localized to minimize bending stress in the ellipsoid geometry defined by the cell wall. Gel phase lipids with high bending rigidity would be spontaneously organized at the equator where curvature is minimal, thus marking the future division site, while liquid phase membrane maps onto the oblong hemispheres. In addition, the membrane-bound cell wall precursor with its particular dynamic acyl chain localizes at the division site where the membrane is highly curved. We propose a complete “chicken-and-egg” model where cell geometry determines the localization of lipid phases that positions the cell division machinery, which in turn alters the localization of lamellar phases by assembling the cell wall with a specific geometry.

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Section: Methodsmentioning

confidence: 99%

Lipid Phases and Cell Geometry During the Cell Cycle of Streptococcus pneumoniae

et al. 2019

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“…Lipid classes were separated and their FA composition was analysed as described by Gros and Jouhet, 2018, with some minor differences. Shortly, after dilution of lipid extract in 1 mL of chloroform, a 50 mL sample was methylated (1 h, 100°C) and the total fatty acids were quantified by GC-FID.…”

Section: Offline Analysismentioning

confidence: 99%

“…The volume corresponding to 750 nmol of FA was loaded in pre-activated Thin Layer Chromatography (TLC) silica plates (105721, Supelco, Germany) and different plates were used to separate polar and neutral lipids, respectively. Migration and identification of lipid classes in TLC plates were done accordingly to Gros and Jouhet, 2018 with the difference that a solution of primuline 0.5% v/v in 8:2 acetone: deionised water was employed as a staining agent. After identification, the silica from the spots was scrapped and collected into separate glass tubes.…”

Section: Offline Analysismentioning

confidence: 99%

Growth and fatty acid distribution over lipid classes in Nannochloropsis oceanica acclimated to different temperatures

et al. 2023

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After light, temperature is the most relevant environmental parameter in outdoors cultivation of microalgae. Suboptimal and supraoptimal temperatures negatively impact growth and photosynthetic performance with a subsequent effect on lipid accumulation. It is generally recognised that lower temperatures trigger an increase in fatty acid desaturation while higher temperatures trigger the opposite reaction. The effect of temperature on lipid classes has been less studied in microalgae and in certain cases, the effect of light cannot be completely excluded. In this research, the effect of temperature on growth, photosynthesis, and lipid class accumulation in Nannochloropsis oceanica was studied at a fixed light gradient with a constant incident light intensity (670 μmol m-2 s-1). A turbidostat approach was used to achieve temperature acclimated cultures of Nannochloropsis oceanica. Optimal growth was found at 25-29°C, while growth was completely arrested at temperatures higher than 31°C and lower than 9°C. Acclimation to low temperatures triggered a decrease in absorption cross section and photosynthesis rates with a tipping point at 17°C. Reduced light absorption was correlated with a decrease in content of the plastid lipids monogalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol. The increase of diacylglyceryltrimethylhomo-serine content at lower temperatures indicated a relevant role of this lipid class in temperature tolerance. Triacylglycerol content increased at 17°C and decreased at 9°C emphasising a metabolic switch in stress response. Total and polar eicosapentaenoic acid content remained constant at 3.5 and 2.4% w/w, despite the fluctuating lipid contents. Results show an extensive mobilisation of eicosapentaenoic acid between polar lipids classes at 9°C to ensure cell survival under critical conditions.

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“…The individual spots corresponding to di erent lipid classes were scratched from the TLC plate and collected separately in a fresh glass tube. Each sample were methylated separately as mentioned above and analysed on GC to obtain the fatty acid pro les of each lipid class [385,386].…”

Section: Thin Layer Chromatography (Tlc) For Neutral Lipid (Nl) and Polar Lipid (Pl) Separationmentioning

confidence: 99%

A “CRISPY” tale on microalgal genome editing: featuring Nannochloropsis oceanica IMET1

Naduthodi

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CHAPTER 1 General Introduction CHAPTER 2 Progress of CRISPR-Cas based genome editing in photosynthetic microbes CHAPTER 3 CRISPR-Cas ribonucleoprotein mediated homology-directed repair for e cient targeted genome editing in microalgae Nannochloropsis oceanica IMET1 CHAPTER 4 A comprehensive genome engineering toolbox for microalgae Nannochloropsis oceanica based on CRISPR-Cas systems CHAPTER 5 High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica CHAPTER 6 Heterologous expression of EPA speci c Lysophosphatidic acid acyltransferase in Nannochloropsis oceanica increases the EPA content in Triglycerides CHAPTER 7 Synthetic biology approaches to enhance microalgal productivity CHAPTER 8 General Discussion References

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Quantitative Assessment of the Chloroplast Lipidome

Cited by 7 publications

References 16 publications

Lipid Phases and Cell Geometry During the Cell Cycle of Streptococcus pneumoniae

Lipid Phases and Cell Geometry During the Cell Cycle of Streptococcus pneumoniae

Growth and fatty acid distribution over lipid classes in Nannochloropsis oceanica acclimated to different temperatures

A “CRISPY” tale on microalgal genome editing: featuring Nannochloropsis oceanica IMET1

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