Quantitative Assessment of Surface-Enhanced Resonance Raman Scattering for the Analysis of Dyes on Colloidal Silver

Abstract

Factors that affect quantitative analysis by surface-enhanced resonance Raman scattering (SERRS) have been investigated using azobenzotriazol and reactive dyes. Preaggregation of the silver colloid was the most effective method to obtain repeatable and reproducible scattering. Aggregation by poly(l-lysine) or spermine provided better precision than aggregation by sodium chloride or nitric acid. Repeatable quantitative analysis was achieved with the azobenzotriazol dyes. A linear calibration graph was obtained over different concentration ranges below 10(-)(8) M, depending on the nature of the colloid. Calculations estimate that 10(-)(8) M is the concentration at which monolayer coverage of the dye on the silver colloid is achieved. Above 10(-)(8) M, there was only a minor increase in the scattering intensity from the azobenzotriazol dyes. In contrast, the reactive dyes did not give a response proportional to concentration over the range studied. The different responses obtained for the two types of dye are believed to be caused by differences in the nature of the interaction of the molecules with the silver surface. The conclusion reached is that control of the colloid preparation, aggregation process, and surface chemistry are essential for successful quantitative analysis of dyes on colloidal silver by SERRS.

Surface enhanced Raman spectra of oligonucleotides induced by spermine

“…Inorganic ions such as H C , Na C , K C , and organic multivalent ions such as poly(L-lysine)/ascorbic acid and spermine have been employed. 21 Selecting a suitable aggregant is an art in developing the full power of SERS and minimizing the complications.…”

Surface‐enhanced Raman scattering of xanthopterin adsorbed on colloidal silver

“…The chemical (charge-transfer) enhancement involves changes in the polarizability of the adsorbate molecule resulting from interaction between the adsorbate and metal surface. 9 Recently, semi-quantitative and quantitative analysis based on SERS have been described, 10,11 and therefore the SERS has received considerable attention in the chemical, environmental and biomedical fields and also in the biology sciences.…”

Selective Trace Analysis of Mercury (II) Ions in Aqueous Media Using SERS-Based Aptamer Sensor

“…23,24 For spermine optimization, 1 µL of 10 µM TAMRA-labelled aptamer was pre-mixed with 5 µL of 100 µM spermine. This solution was then mixed with 200 µL of silver nanocolloids.…”