Quantitative Assessment of Regional Myocardial Function in Mice by Tissue Doppler Imaging

Sebag, Igal A.; Handschumacher, Mark D.; Ichinose, Fumito; Morgan, John G.; Hataishi, Ryuji; Rodrigues, Ana Clara T.; Guerrero, J. Luis; Steudel, Wolfgang; Raher, Michael J.; Halpern, Elkan F.; Dérumeaux, Geneviève; Bloch, Kenneth D.; Picard, Michael H.; Scherrer‐Crosbie, Marielle

doi:10.1161/circulationaha.104.474411

Cited by 91 publications

(94 citation statements)

References 33 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3,5,13 Doppler echocardiography has been widely used for the estimation of LV function in recent years. 3,5,6,8,11,19 However, there is a little information on the characterization of LV wall structure and formation by ultrasound in a rat MI model, particularly pertaining to MI size. Earlier studies 20,21 found no difference in the estimated MI size compared with histologic findings, and in another study, 22 the infarcted short-axis LVAWT was observed to be significantly thinner because of scarring than in control rats, and FS was also different, whereas the LVPWT was similar to that in the control animals.…”

Section: Discussionmentioning

confidence: 99%

Wideband High-Frequency Echocardiography to Evaluate Myocardial Infarct Size

Zhang

Khanna

2009

Journal of Ultrasound in Medicine

View full text Add to dashboard Cite

Objective. This study was designed to validate the feasibility of wideband high-frequency ultrasound imaging to resolve in vivo the degree, location, and morphologic changes of myocardial infarction (MI) in a rat model. Methods. The left anterior descending coronary artery was ligated in the test group (n = 41), and the sham control group did not have ligation (n = 7). The rats were examined with 10-to 22-MHz echocardiography to evaluate the MI size, location, and geometric formation. Results. The endocardial chamber shape was deformed, with enlargement of the anteroposterior dimension and fractional shortening, and was comparable with the degree of MI both in short-and long-axis sections of the left ventricle. Histologic analysis showed remodeling to different extents corresponding to different MI sizes (small, medium, and large). Conclusions. The results suggest that this technique can be used in vivo to evaluate the MI location, size, and morphologic changes corresponding to the extent of the injury. Key words: high-frequency ultrasound imaging; histologic analysis; left anterior descending coronary artery; myocardial infarction. he rat is a preferred species for studying the pathophysiologic mechanisms of myocardial infarction (MI), cardiomyopathy, and other cardiovascular diseases. 1,2 Noninvasive in vivo study of rat cardiac structure and function has attracted interest in cardiovascular research, especially those involving the left ventricle (LV). The rat infarct model has been used extensively to study LV dysfunction after MI and in the evaluation of experimental therapies for heart failure. 3,4 Two-dimensional (2D) B-mode, M-mode, and Doppler ultrasound have been used to evaluate LV structural and functional remodeling in humans for decades, particularly in patients with MI during the acute phase and for later follow-up. However, the small size and rapid heart rate (300-500 beats per minute) of the rat heart demand an imaging modality with both high temporal resolution and high spatial resolution. 5,6 Recently developed high-resolution echocardiographic systems with high frequencies (>20 MHz) are particularly well suited for the study of the heart in rodent models of MI. These systems can be used for evaluating the heart contractility, mainly LV systolic and diastolic function.

show abstract

Section: Discussionmentioning

confidence: 99%

Wideband High-Frequency Echocardiography to Evaluate Myocardial Infarct Size

Zhang

Khanna

2009

Journal of Ultrasound in Medicine

View full text Add to dashboard Cite

show abstract

“…Strain is a good parameter for regional and global systolic function, whereas strain rate is a good parameter for diastolic regional and global function. Sebag et al 37 could nicely demonstrate the value of strain and strain rate imaging in mice in a murine sepsis model of acute heart failure. Since myocardial contraction, myocardial stiffness and myocardial ............................................................................................................................................. compliance are closely dependent on sufficient blood and, thus, on adequate oxygen supply, strain and strain rate indicate something about regional and global systolic and diastolic LV function.…”

Section: Strain and Strain Ratementioning

confidence: 99%

“…To the best of our knowledge, up-to-date data on strain and strain rate in mice have only been published in parasternal short-axis views. 37 Passive changes of tissue position caused by tethering lead to displacement which commonly is normalized to the distance from the LV apex to get rid of the cumulative effect of all myocardial contractions along the longitudinal LV wall from base to apex ( Figure 5A). Strain, by definition, is relative deformation indicating how much the myocardial tissue has deformed ( Figure 5B).…”

Section: Strain and Strain Ratementioning

confidence: 99%

Echocardiographic assessment of global left ventricular function in mice

et al. 2009

View full text Add to dashboard Cite

Doppler-echocardiographic assessment of cardiovascular structure and function in murine models has developed into one of the most commonly used non-invasive techniques during the last decades. Recent technical improvements even expanded the possibilities. In this review, we summarize the current options to assess global left ventricular (LV) function in mice using echocardiographic techniques. In detail, standard techniques as structural and functional assessment of the cardiovascular phenotype using one-dimensional M-mode echocardiography, two-dimensional B-mode echocardiography and spectral Doppler signals from mitral inflow respective aortal outflow are presented. Further pros and contras of recently implemented techniques as three-dimensional echocardiography and strain and strain rate measurements are discussed. Deduced measures of LV function as the myocardial performance index according to Tei, estimation of the mean velocity of circumferential fibre shortening, LV wall stress and different algorithms to estimate the LV mass are described in detail. Last but not least, specific features and limitations of murine echocardiography are presented. Future perspectives in respect to new examination techniques like targeted molecular imaging with advanced ultrasound contrast bubbles or improvement of equipment like new generation matrix transducers for murine echocardiography are discussed.

show abstract

“…Other indices for quantifying global and regional leftventricular function can be obtained with Doppler tissue imaging, which measures blood flow velocities and strain rates, both of which have been shown to correlate with the maximum rate of rise of left-ventricular pressure, or dP/dt max , an indicator of myocardial contractility (94). Some studies have used more invasive procedures such as pressure-volume loop analysis to directly measure hemodynamic parameters (38,95).…”

Section: Hemodynamic Assessmentmentioning

confidence: 99%