“…For Synechococcus, the probes used targeted the following specific lineages: clade I (SYN1006), clade II (SYN1006RS), clade III (SYN262), clade IV (SYN635) or more generally clades I-VII and X (SYN1258). The nuclear 18S rRNA oligonucleotide probes used were EUK1209r, NCHLO01 and CHLO02 to target all eukaryotes and the class-specific probes PRAS01, PRAS02, PRAS05 (targeting prasinophytes), and CRYPTO13, PRYM02 and PELA01 targeting cryptophytes, prymnesiophytes and pelagophytes, respectively (Giovannoni et al, 1988;Simon et al, 1995Simon et al, , 2000Biegala et al, 2003;Not et al, 2002Not et al, , 2004. The chrysophytespecific probe (CHRYSO1037) targeted the chloroplast 16S rRNA gene, as used previously for dot blot hybridization analysis (Fuller et al, 2006b) and validated here for tyramide signal amplification (TSA)-FISH analysis (see below).…”